Morphological variation in Tricyrtis hirta plants regenerated from heavy ion beam-irradiated embryogenic calluses

Nakano, Masaru; Amano, Junji; Watanabe, Yusuke; Nomizu, Toshikazu; Suzuki, Mami; Mizunashi, Keiko; Mori, Satoshi; Kuwayama, Sachiko; Han, Dongsheng; Saito, Hiroyuki; Ryuto, Hiromichi; Fukunishi, Nobuhisa; Abe, Tomoko

doi:10.5511/plantbiotechnology.27.155

Cited by 16 publications

(11 citation statements)

References 15 publications

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“…Hence, the heavy-ion beam provides a higher relative biological effectiveness (RBE) than the low LET radiation (Tanaka et al, 2010 ; Kazama et al, 2011 ; Nagata et al, 2016 ; Zhou et al, 2016 ). For plant mutation breeding, various mutant populations have been generated by heavy-ion beam irradiation: for instance, those of Arabidopsis thaliana (Tanaka et al, 1997 , 2002 ), Lotus japonicus (Oka-Kira et al, 2005 ; Luo et al, 2016 ), Rice ( Oryza sativa L.) (Ishikawa et al, 2012 ; Phanchaisri et al, 2012 ; Morita et al, 2017 ), Petunia ( Petunia hybrid ), Hase et al, 2010 ), Tricyrtis hirta (Nakano et al, 2010 ), Chrysanthemum (Matsumura et al, 2010 ), Wandering Jew (He et al, 2011 ), Geranium (Yu et al, 2016 ), and so on.…”

Section: Introductionmentioning

confidence: 99%

Identification of Substitutions and Small Insertion-Deletions Induced by Carbon-Ion Beam Irradiation in Arabidopsis thaliana

Luo

et al. 2017

Front. Plant Sci.

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Heavy-ion beam irradiation is one of the principal methods used to create mutants in plants. Research on mutagenic effects and molecular mechanisms of radiation is an important subject that is multi-disciplinary. Here, we re-sequenced 11 mutagenesis progeny (M3) Arabidopsis thaliana lines derived from carbon-ion beam (CIB) irradiation, and subsequently focused on substitutions and small insertion-deletion (INDELs). We found that CIB induced more substitutions (320) than INDELs (124). Meanwhile, the single base INDELs were more prevalent than those in large size (≥2 bp). In details, the detected substitutions showed an obvious bias of C > T transitions, by activating the formation of covalent linkages between neighboring pyrimidine residues in the DNA sequence. An A and T bias was observed among the single base INDELs, in which most of these were induced by replication slippage at either the homopolymer or polynucleotide repeat regions. The mutation rate of 200-Gy CIB irradiation was estimated as 3.37 × 10−7 per site. Different from previous researches which mainly focused on the phenotype, chromosome aberration, genetic polymorphism, or sequencing analysis of specific genes only, our study revealed genome-wide molecular profile and rate of mutations induced by CIB irradiation. We hope our data could provide valuable clues for explaining the potential mechanism of plant mutation breeding by CIB irradiation.

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Section: Introductionmentioning

confidence: 99%

Identification of Substitutions and Small Insertion-Deletions Induced by Carbon-Ion Beam Irradiation in Arabidopsis thaliana

Luo

et al. 2017

Front. Plant Sci.

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“…Mutation induction and selection of mutants have been powerful tools for plant breeding as well as for physiological and molecular studies for the past 80 years. X-ray, gamma ray irradiation, and chemical treatments have been used for mutation breeding in a wide range of plants [ 3 ]. Gamma rays are a type of ionizing radiation which interacts with atoms or molecules to produce free radicals in cells which damage or modify important components of plant cells and affect differently the morphology, biochemistry, and physiology of plants.…”

Section: Introductionmentioning

confidence: 99%

Effect of Acute Gamma Irradiation onCurcuma alismatifoliaVarieties and Detection of DNA Polymorphism through SSR Marker

Taheri

Abdullah

Ahmad

et al. 2014

BioMed Research International

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The effects of eight different doses (0, 10, 20, 25, 35, 40, 60, and 100 Gy) of acute gamma irradiation on 44 (three varieties of Curcuma alismatifolia: Chiang Mai Red, Sweet Pink, Kimono Pink, and one Curcuma hybrid (Doi Tung 554) individual plants were investigated. Radiation sensitivity tests revealed that the LD50 values of the varieties were achieved at 21 Gy for Chiang Mai Red, 23 Gy for Sweet Pink, 25 Gy for Kimono Pink, and 28 Gy for Doi Tung 554. From the analysis of variance (ANOVA), significant variations were observed for vegetative traits, flowering development, and rhizome characteristics among the four varieties of Curcuma alismatifolia and dose levels as well as the dose × variety interaction. In irradiated plants, the leaf length, leaf width, inflorescence length, the number of true flowers, the number of pink bracts, number of shoots, plant height, rhizome size, number of storage roots, and number of new rhizomes decreased significantly (P < 0.05) as the radiation dose increased. The cophenetic correlation coefficient (CCC) between genetic dissimilarity matrix estimated from the morphological characters and the UPGMA clustering method was r = 0.93, showing a proof fit. In terms of genetic variation among the acutely irradiated samples, the number of presumed alleles revealed by simple sequence repeats ranged from two to seven alleles with a mean value of 3.1, 4.5, and 5.3 alleles per locus for radiation doses of 0, 10, and 20 Gy, respectively. The average values of the effective number of alleles, Nei's gene diversity, and Shannon's information index were 2.5–3.2, 0.51–0.66, and 0.9–1.3, respectively. The constructed dendrogram grouped the entities into seven clusters. Principal component analysis (PCA) supported the clustering results. Consequently, it was concluded that irradiation with optimum doses of gamma rays efficiently induces mutations in Curcuma alismatifolia varieties.

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“…Somaclonal mutants have provided valuable genetic resources for plant breeding. However, although it is possible to select mutants by somaclonal variation, mutant frequencies are often low compared with artificial mutagenesis employing such methods as ionizing radiation and chemical treatments [23].…”

Section: Introductionmentioning

confidence: 99%

The improvement of ginsenoside accumulation in Panax ginseng as a result of γ-irradiation

Kim¹,

Song²,

Kim³

et al. 2013

Journal of Ginseng Research

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In this study, gamma rays were used to irradiate embryogenic calli induced from cotyledon explants of Panax ginseng Meyer. After the embryogenic calli were irradiated, they were transferred to adventitious roots using an induction medium; next, mutated adventitious root (MAR) lines with a high frequency of adventitious root formations were selected. Two MAR lines (MAR 5-2 and MAR 5-9) from the calli treated with 50 Gy of gamma rays were cultured on an NH4NO3-free Murashige and Skoog medium with indole-3-butyric acid 3 mg/L. The expression of genes related to ginsenoside biosynthesis was analyzed using reverse transcription polymerase chain reaction with RNA prepared from native ginseng (NG), non-irradiated adventitious root (NAR) and 2 MAR lines. The expression of the squalene epoxidase and dammarenediol synthase genes was increased in the MAR 5-2 line, whereas the phytosterol synthase was increased in the MAR 5-9 line. The content and pattern of major ginsenosides (Rb1, Rb2, Rc, Rd, Re, Rf, and Rg1) were analyzed in the NG, NAR, and 2 MAR lines (MAR 5-2 and MAR 5-9) using TLC and HPLC. In the TLC analysis, the ginsenoside patterns in the NG, NAR, and 2 MAR lines were similar; in contrast, the MAR 5-9 line showed strong bands of primary ginsenosides. In the HPLC analysis, compared with the NG, one new type of ginsenoside was observed in the NAR and 2 MAR lines, and another new type of ginsenoside was observed in the 2 MAR lines irradiated with gamma rays. The ginsenoside content of the MAR 5-9 line was significantly greater in comparison to the NG.

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Morphological variation in Tricyrtis hirta plants regenerated from heavy ion beam-irradiated embryogenic calluses

Cited by 16 publications

References 15 publications

Identification of Substitutions and Small Insertion-Deletions Induced by Carbon-Ion Beam Irradiation in Arabidopsis thaliana

Identification of Substitutions and Small Insertion-Deletions Induced by Carbon-Ion Beam Irradiation in Arabidopsis thaliana

Effect of Acute Gamma Irradiation onCurcuma alismatifoliaVarieties and Detection of DNA Polymorphism through SSR Marker

The improvement of ginsenoside accumulation in Panax ginseng as a result of γ-irradiation

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