Morphological Study on Alloxan-Induced Cataract

Sallmann, L.; Caravaggio, Leo L.; Grimes, Patricia A.; Collins, Eleanor M.

doi:10.1001/archopht.1958.00940020081008

Cited by 40 publications

(4 citation statements)

References 15 publications

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“…The proliferative reaction on the Y suture started after 4 Cytological constituents of the Y reaction and posterior evolution have been analyzed pre¬ viously (Fig. 11, A-3 (Fig.…”

Section: Introductionmentioning

confidence: 99%

The Mitotic Cycle of the Lens Epithelium

Cotlier¹

1962

Arch Ophthalmol

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“…The proliferative reaction on the Y suture started after 4 Cytological constituents of the Y reaction and posterior evolution have been analyzed pre¬ viously (Fig. 11, A-3 (Fig.…”

Section: Introductionmentioning

confidence: 99%

The Mitotic Cycle of the Lens Epithelium

Cotlier¹

1962

Arch Ophthalmol

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“…In such a study, phases in the transformation of lens epithelial cells to fibers can be observed as well as the subsequent regressive metamorphosis which they undergo during their gradual displacement towards the lens nucleus. Moreover, the normal morphology of the lens bow area is of interest as this is the site of early pathological changes in most forms of experimental and h u m a n cataract (1)(2)(3).…”

Section: Introductionmentioning

confidence: 99%

Electron Microscope Study of Lens Fibers

Wanko

Gavin

1959

The Journal of Cell Biology

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PLATES 58 TO 62(Received for publication, January 16, 1959) ABSTRACT Comparative electron microscope investigations on sections of the lens cortex of the normal, mature rat, rabbit, monkey, and the normal calf reveal similar patterns of intracellular organization.The superficial lens fiber contains a nucleus, mitochondria, endoplasmic reticulum, dense granules, Golgi complex, and a quantity of small structures of low opacity which appear as filamentous and spherical configurations.Variations in number, distribution, and spatial arrangement of cytoplasmic elements in lens fibers are described. These changes in the pattern of cytoplasmic organization are concomitant with development of fibers and their displacement towards the center of the lens. Structural details of the various zones of the lens epithelium and the lens fibers are compared.

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“…Here, we examined quadriceps from diabetic and normal control rabbits 16 weeks after onset of diabetes and hyperglycemia. Studies of diabetic complications in other organs using this model indicated a time frame of 4-6 mos produced substantial effects [38,39]. We identified substantial increases in PrP, SOD1, NOS1, and NOS2 expression on immunoblots of total muscle protein samples from diabetic animals, and ELISA assays measured several fold increases in Aβ peptides produced in diabetic muscle.…”

Section: Introductionmentioning

confidence: 78%

Increased expression and local accumulation of the Prion Protein, Alzheimer Aβ peptides, superoxide dismutase 1, and Nitric oxide synthases 1 & 2 in muscle in a rabbit model of diabetes

et al. 2010

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BackgroundMuscle disease associated with different etiologies has been shown to produce localized accumulations of amyloid and oxidative stress-related proteins that are more commonly associated with neurodegeneration in the brain. In this study we examined changes in muscle tissue in a classic model of diabetes and hyperglycemia in rabbits to determine if similar dysregulation of Alzheimer Aβ peptides, the prion protein (PrP), and superoxide dismutase 1 (SOD1), as well as nitric oxide synthases is produced in muscle in diabetic animals. This wild-type rabbit model includes systemic physiological expression of human-like Alzheimer precursor proteins and Aβ peptides that are considered key in Alzheimer protein studies.ResultsDiabetes was produced in rabbits by injection of the toxic glucose analogue alloxan, which selectively enters pancreatic beta cells and irreversibly decreases insulin production, similar to streptozotocin. Quadriceps muscle from rabbits 16 wks after onset of diabetes and hyperglycemia were analyzed with biochemical and in situ methods. Immunoblots of whole muscle protein samples demonstrated increased PrP, SOD1, as well as neuronal and inducible Nitric oxide synthases (NOS1 and NOS2) in diabetic muscle. In contrast, we detected little change in Alzheimer Aβ precursor protein expression, or BACE1 and Presenilin 1 levels. However, Aβ peptides measured by ELISA increased several fold in diabetic muscle, suggesting a key role for Aβ cleavage in muscle similar to Alzheimer neurodegeneration in this diabetes model. Histological changes in diabetic muscle included localized accumulations of PrP, Aβ, NOS1 and 2, and SOD1, and evidence of increased central nuclei and cell infiltration.ConclusionsThe present study provides evidence that several classic amyloid and oxidative stress-related disease proteins coordinately increase in overall expression and form localized accumulations in diabetic muscle. The present study highlights the capacity of this wild-type animal model to produce an array of hallmark pathological features that have also been described in other muscle diseases.

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Morphological Study on Alloxan-Induced Cataract

Cited by 40 publications

References 15 publications

The Mitotic Cycle of the Lens Epithelium

The Mitotic Cycle of the Lens Epithelium

Electron Microscope Study of Lens Fibers

Increased expression and local accumulation of the Prion Protein, Alzheimer Aβ peptides, superoxide dismutase 1, and Nitric oxide synthases 1 & 2 in muscle in a rabbit model of diabetes

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