Morphological Study of the Liver in the Teleost Oreochromis niloticus

Vicentini, Carlos Alberto; Franceschini-Vicentini, Irene Bastos; Bombonato, Maria Terezinha Siqueira; Bertolucci, Bruno; Lima, Silvana Garcia de Andrade; Santos, Allisson Silva dos

doi:10.4067/s0717-95022005000300003

Cited by 37 publications

(39 citation statements)

References 7 publications

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“…These findings are similar to other teleosts (Akiyoshi, Inoue, 2004;Bombonato et al, 2007). The bile ducts observed in the three species had a simple cubic epithelium, which becomes columnar in large ducts similar to other teleosts (Vicentini et al, 2005;Faccioli et al, 2014). Only G. brasiliensis has an intrahepatic exocrine pancreas that was arranged in clusters of columnar cells, which were organized in acini.…”

Section: Discussionsupporting

confidence: 73%

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Comparative histology in the liver and spleen of three species of freshwater teleost

et al. 2017

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We describe and compare the histology of liver and spleen of Geophagus brasiliensis (Perciformes), Hypostomus francisci (Siluriformes) and Hoplias aff. malabaricus (Characiformes), tropical freshwater fishes. In G. brasiliensis and H. aff. malabaricus the hepatocytes were arranged in tubular form whereas in H. francisci they cord-like. In all species, hepatocytes presented glycogen, but in G. brasiliensis and H. aff. malabaricus they showed strong stained for hemossiderin in the cytoplasm. In G. brasiliensis and H. aff. malabaricus, melanomacrophage centres (MMCs) were associated to hepatic structures and only in G. brasiliensis was observed intrahepatic exocrine pancreas. The spleen, in all species, was characterized by red and white pulp without boundary between the two regions, but only in H. francisci was recorded nodular organization in splenic parenchyma. The G. brasiliensis and H. aff. malabaricus presented in the white pulp MMCs linked mainly to ellipsoids. Besides, we observed large MMCs in the spleen in relation to liver of G. brasiliensis and H. aff. malabaricus. In liver, highest values of reticular fibers and collagen were observed in G. brasiliensis. In spleen, highest values of reticular fibers and collagen were recorded in H. aff. malabaricus and H. francisci, respectively. Histological differences confirm the hypothesis that the phylogenetic distance is reflected in liver and spleen.Keywords: Extracellular matrix, Hepatocytes, Intrahepatic exocrine pancreas, Melanomacrophage centres, Red and White pulp.Nós descrevemos e comparamos a histologia do fígado e do baço de Geophagus brasiliensis (Perciformes), Hypostomus francisci (Siluriformes) e Hoplias aff. malabaricus (Characiformes), peixes neotropicais de água doce. Em G. brasiliensis e H. aff. malabaricus os hepatócitos organizaram-se na forma tubular enquanto que em H. francisci eles apresentaram-se como cordões celulares. Em todas as espécies, os hepatócitos apresentaram glicogênio, mas em G. brasiliensis e H. aff. malabaricus, eles mostraram forte marcação para hemossiderina no citoplasma. Em G. brasiliensis e H. aff. malabaricus, centros melanomacrofágicos (CMMs) foram associados a estruturas hepáticas e somente em G. brasiliensis foi observado pâncreas exócrino intrahepático. O baço, em todas as espécies, foi caracterizado pela polpa vermelha e branca sem limites entre as duas regiões, mas somente em H. francisci foi registrado uma organização nodular no parênquima esplênico. G. brasiliensis e H. aff. malabaricus apresentaram na polpa branca CMMs associados principalmente a elipsoides. Além disso, nós observamos CMMs grandes no baço em relação ao fígado de G. brasiliensis e de H. aff. malabaricus. No fígado, valores altos de fibras reticulares e colágeno foram observado em G. brasiliensis. No baço, valores altos de fibras reticulares e colágeno foram registrados em H. aff. malabaricus e H. francisci, respectivamente. Diferenças histológicas confirmam a hipótese que a distância filogenética está refletida no fígado e no baço. Palavras-chave:...

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Section: Discussionsupporting

confidence: 73%

“…In the present study, three species had absence of division into hepatic lobules and the lack of portal triads as also recorded in many teleosts (Vicentini et al, 2005;Nejedli, Gajger, 2013;Pronina et al, 2014;Faccioli et al, 2014). The network of blood capillaries showed vessels that were anastomosed and drained by centre-lobular veins.…”

Section: Discussionmentioning

confidence: 78%

Comparative histology in the liver and spleen of three species of freshwater teleost

et al. 2017

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“…Animal Sciences Maringá, v. 39, n. 4, p. 343-349, Oct.-Dec., 2017 In the presence of hepatopancreatic tissue in pancreatic acinar cells containing secretory granules numerous eosinophils was also observed. The results of this study fit to the description of liver morphology of teleost and to Nile tilapia (Vicentini et al, 2005). The cytoplasm vacuolization indicates the probable location of lipid and glycogen reserves in hepatocytes.…”

Section: Resultssupporting

confidence: 56%

“…The average dissolved oxygen in experimental tanks was equal to 6.9 ± 1.5 mg L At the end of the experiment, the fish were anesthetized (eugenol 75 mg l -¹) (Moreira, Teixeira, Carreiro & Moreira, 2010), weighed and measured (height, width, length and head) individually for the assessment of body parameters, chemical composition of the carcass and liver histology. In each experimental unit three animals were selected and randomly slaughtered to assessment of body yield parameters and histological evaluation (Vicentini et al, 2005) For the study of the morphology and integrity of the hepatic tissue, the procedures described by Graciano, Natali, Vidal, Michelato, Righetti and Furuya (2010) The final results of the evaluated parameters were submitted to variance analysis with significance level of 5% and in the case of statistical difference were applied regression analysis, using the SAEG program. vit.…”

Section: Methodsmentioning

confidence: 99%

<b>Digestible protein requirement for Nile tilapia fed with rations based on soybean meal and corn

Carneiro¹,

Pandini²,

Silva³

et al. 2017

Acta Sci. Anim. Sci.

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ABSTRACT. The objective was to determine the requirement of digestible protein (DP) for Nile tilapia fed with diets based on soybean meal and corn. Two hundred Nile tilapia juveniles, sexually reversed, were used for a trial period of 100 days. The animals were distributed in 20 boxes of 1000 L -1 in a random design with five treatments and four replications, the boxes were connected to a water recirculation system. Five isoenergetic diets were tested with different levels of digestible protein (DP): 20, 25, 30, 35 and 40%. At the end of the experimental period were evaluated the zootechnical performance data. A quadratic effect (p < 0.05) was shown to parameters of final weight, weight gain, final length, fillet yield and visceral fat with the best points that ranged from 28.3 to 29.9% of DP. The largest area of hepatocytes was found to the lowest levels of DP in the diet (20 and 25%) compared to the other levels, which were similar. Thus, Nile tilapia has adequate performance for the consumption of diets based on soybean and corn meal and the recommended level is 28.3% of PD in these conditions. Keywords: plant origin feed, aquaculture, fish nutrition.Exigência de proteína digestível para tilápias do Nilo alimentadascom rações à base de farelo de soja e milho RESUMO. Objetivou-se determinar a exigência de proteína digestível (PD) para a tilápia do Nilo, alimentada com dietas à base de farelo de soja e milho. Duzentos juvenis de tilápia do Nilo (32,80 ± 0,39 g), sexualmente revertidos, foram utilizados por um período experimental de 100 dias. Os animais foram distribuídos em 20 caixas de 1000L -1 em delineamento aleatório com cinco tratamentos e quatro repetições. As caixas eram ligadas a um sistema de recirculação de água. Foram testadas cinco dietas isoenergéticas com diferentes níveis de PD: 20, 25, 30, 35 e 40%. Ao final do período experimental, foram avaliados os dados de desempenho zootécnico. Um efeito quadrático (p < 0,05) foi mostrado aos parâmetros de peso final, ganho em peso, comprimento final, rendimento de filé e de gordura visceral com os melhores pontos que variaram de 28,3 a 29,9% de PD. A maior área dos hepatócitos foi encontrada para os níveis mais baixos de PD na dieta (20 e 25%), em comparação com os outros níveis, que foram semelhantes. Assim, pode-se afirmar que a tilápia do Nilo tem desempenho adequado para o consumo de dietas baseadas em farelo de soja e milho e o nível recomendado é de 28,3% PD nestas condições.Palavras-chave: alimentos de origem vegetal, aquicultura, nutrição de peixes.

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“…In the liver parenchyma of H. platyrhynchos, the hepatocytes are arranged in cords and it was no division in lobules and portal triads was observed, as evidenced in other teleosts (Kendall & Hawkins;Hampton et al, 1985;Gonzalez et al, 1993;Vicentini et al, 2005;Bombonato et al;Bertolucci et al). The hepatocytes of H. platyrhynchos are polygonal and present basal nucleus and apical cytoplasm with few glycogen granules but large amount of lipids, similar to observed in Sparus aurata (El-Bakary & El-Gammal, 2010).…”

Section: Discussionmentioning

confidence: 99%

Morphology and Histochemistry of the Liver of Carnivorous Fish Hemisorubim platyrhynchos

Faccioli¹,

Chedid

Bombonato³

et al. 2014

Int. J. Morphol.

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SUMMARY:The aim of this study was to characterize the normal structure of the liver of Hemisorubim platyrhynchos, a carnivorous freshwater catfish found in Neotropical region, using gross anatomy, histology and histochemistry. Anatomically, the liver presents C-shaped and only two lobes: smaller right and bigger left. The gallbladder is located in right lobe and shows elongated shaped. Histological analysis demonstrated that the hepatic parenchyma is made of two hepatocytes plates surrounded by sinusoids. The hepatocytes are polygonal-shaped cells, with spherical nucleus and a dark prominent nucleolus. The cytoplasm presents large amount of lipids and glycogen deposits PAS positives. There are no hepatic lobules or portal triads. Bile ducts are lined by columnar epithelial cells with apical mucosubstances PAS and AB positive. Furthermore, the liver presents melano-macrophages centers, distributed next to the blood vessels and bile ducts, constituted by cells accumulating pigments, whose presence may be related to the nutritional status of the fish. Moreover pancreatic tissue was observed in visceral portion of liver, formed by exocrine pancreatic tissue and islet organ, constituting an extrahepatic pancreas.

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Morphological Study of the Liver in the Teleost Oreochromis niloticus

Cited by 37 publications

References 7 publications

Comparative histology in the liver and spleen of three species of freshwater teleost

Comparative histology in the liver and spleen of three species of freshwater teleost

<b>Digestible protein requirement for Nile tilapia fed with rations based on soybean meal and corn

Morphology and Histochemistry of the Liver of Carnivorous Fish Hemisorubim platyrhynchos

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