Morphological Study of Surgically Obtained Human Cochlear Specimens - Technical Aspects

Liu, Wei; Guo, Rui; Helge, Rask Andersen

doi:10.1016/s1672-2930(14)50010-4

Cited by 2 publications

(2 citation statements)

References 24 publications

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“…The proposed methodology has been validated using a qualitative and a quantitative validation approach. More specifically, first we compared the reconstructed geometries qualitatively with specimens of the existed literature [10,26,27]. We also compare the geometries with the previously reconstructed geometries of Braun et al [17].…”

Section: Qualitative and Quantitative Validation Of The Proposed Meth...mentioning

confidence: 99%

A validated methodology for the 3D reconstruction of cochlea geometries using human microCT images

Sakellarios

Tachos

Rigas

et al. 2017

Meas. Sci. Technol.

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Accurate reconstruction of the inner ear is a prerequisite for the modelling and understanding of the inner ear mechanics. In this study, we present a semi-automated methodology for accurate reconstruction of the major inner ear structures (scalae, basilar membrane, stapes and semicircular canals). For this purpose, high resolution microCT images of a human specimen were used. The segmentation methodology is based on an iterative level set algorithm which provides the borders of the structures of interest. An enhanced coupled level set method which allows the simultaneous multiple image labeling without any overlapping regions has been developed for this purpose. The marching cube algorithm was applied in order to extract the surface from the segmented volume. The reconstructed geometries are then post-processed to improve the basilar membrane geometry to realistically represent physiologic dimensions. The final reconstructed model is compared to the available data from the literature. The results show that our generated inner ear structures are in good agreement with the published ones, while our approach is the most realistic in terms of the basilar membrane thickness and width reconstruction.

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Section: Qualitative and Quantitative Validation Of The Proposed Meth...mentioning

confidence: 99%

A validated methodology for the 3D reconstruction of cochlea geometries using human microCT images

Sakellarios

Tachos

Rigas

et al. 2017

Meas. Sci. Technol.

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“…The exact similarities and differences between inner ear tissues from various species compared to human tissues remain unclear. Current sources of human inner ear tissues are primarily from patients undergoing surgical resection for lateral skull base pathologies (e.g., meningiomas, vestibular schwannoma) (8)(9)(10)(11), aborted fetuses (12)(13)(14), post mortem cadavers (15), as well as from libraries of archived temporal bones (16). Patients with lateral skull base pathologies warranting surgical resection typically have hearing loss, with inner ear organs displaying hair cell loss (17).…”

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confidence: 99%

Selection Criteria Optimal for Recovery of Inner Ear Tissues From Deceased Organ Donors

Aaron

Hosseini

Vaisbuch

et al. 2022

Otology &Amp; Neurotology

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Objective:To identify optimal conditions for recovering viable inner ear tissues from deceased organ donors.Setting:Tertiary recovery hospitals and Donor Network West Organ Recovery Center.Interventions:Recovering bilateral inner ear tissues and immunohistological analysis.Main Outcome Measures:Immunohistochemical analysis of utricles from human organ donors after brain death (DBD) or donors after cardiac death (DCD).Results:Vestibular tissues from 21 organ donors (39 ears) were recovered. Of these, 18 donors (33 utricles) were examined by immunofluorescence. The sensory epithelium was present in seven utricles (two from DBD and five from DCD). Relative to DBD utricles, DCD organs more commonly displayed dense populations of hair cells and supporting cells. Relative to DBD, DCD had significantly shorter postmortem interval time to tissue recovery (<48 h). Compared to donors with no sensory epithelium, donors with intact and viable sensory epithelium (both DCD and DBD) had significantly shorter lag time to resuscitation prior to hospital admission (6.4 ± 9.2 vs 35.6 ± 23.7 min, respectively) as well as a shorter time between pronouncements of death to organ recovery (22.6 ± 30.4 vs 64.8 ± 22.8 h, respectively).Conclusions:Organ donors are a novel resource for bilateral inner ear organs. Selecting tissue donors within defined parameters can optimize the quality of recovered inner ear tissues, thereby facilitating future research investigating sensory and nonsensory cells.

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Morphological Study of Surgically Obtained Human Cochlear Specimens - Technical Aspects

Cited by 2 publications

References 24 publications

A validated methodology for the 3D reconstruction of cochlea geometries using human microCT images

A validated methodology for the 3D reconstruction of cochlea geometries using human microCT images

Selection Criteria Optimal for Recovery of Inner Ear Tissues From Deceased Organ Donors

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