Morphological studies of the epiphyseal growth zone in the brachymorphic (bm/bm) mouse

Wikström, B.; Hjerpe, Anders; Mengarelli, Silwa; Reinholt, Finn P.; Engfeldt, Bengt

doi:10.1007/bf02890199

Cited by 19 publications

(5 citation statements)

References 14 publications

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“…The Pe and Co of the RGDS‐injected limbs were less densely distributed than in the control limbs, and there were fewer BrdU‐positive cells per unit of cartilage area in both the proximal and distal cartilage areas. It was reported that the percentages of nuclear profiles in the proliferating zone were lower in the tibias of brachymorphic (bm/bm) mice than in those of controls, and that the total height of the plate was reduced by more than one‐third in bm/bm mice . The present study suggested that cartilage was shortened by the decrease in chondrocyte proliferation.…”

Section: Discussionsupporting

confidence: 52%

“…It was reported that the percentages of nuclear profiles in the proliferating zone were lower in the tibias of brachymorphic (bm/bm) mice than in those of controls, and that the total height of the plate was reduced by more than one-third in bm/bm mice. (36,37) The present study suggested that cartilage was shortened by the decrease in chondrocyte proliferation. Furthermore, the relative expression levels of protein and mRNA of Coll 2, the primary component of the ECM in articular cartilage tissue, were lower in the RGDS-injected limbs than in the control limbs.…”

Section: Discussionsupporting

confidence: 49%

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In Vivo Analysis of Arg-Gly-Asp Sequence/Integrin α5β1-Mediated Signal Involvement in Embryonic Enchondral Ossification by Exo Utero Development System

Inoue

Hashimoto

Matsumoto

et al. 2013

Journal of Bone and Mineral Research

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Enchondral ossification is a fundamental mechanism for longitudinal bone growth during vertebrate development. In vitro studies suggested that functional blockade with RGD peptides or with an antibody that interferes with integrin a5b1-ligand interactions inhibited pre-hypertrophic chondrocyte differentiation. The purpose of this study is to elucidate in vivo the roles of the integrin a5b1-mediated signal through the Arg-Gly-Asp (RGD) sequence in the cell-extracellular matrix (ECM) interaction in embryonic enchondral ossification by an exo utero development system. We injected Arg-Gly-Asp-Ser (RGDS) peptides and anti-integrin a5b1 antibody (a5b1 ab) in the upper limbs of mouse embryos at embryonic day (E) 15.5 (RGDS-injected limbs, a5b1 ab-injected limbs), and compared the effects on enchondral ossification with those found in the control limbs (Arg-Gly-Glu-Ser peptide-, mouse IgG-, or vehicle-injected, and no surgery) at E16.5. In the RGDS-injected limbs, the humeri were shorter and there were fewer BrdU-positive cells than in the control limbs. The ratios of cartilage length and area to those of the humerus were higher in the RGDS-injected limbs. The ratios of type X collagen to type 2 collagen mRNA and protein (Coll X/Coll 2) were significantly lower in the RGDS-injected limbs. In those limbs, TUNEL-positive cells were hardly observed, and the ratios of fractin to the Coll X/Coll 2 ratio were lower than in the control limbs. Furthermore, the a5b1 ab-injected limbs showed results similar to those of RGDS-injected limbs. The present in vivo study by exo utero development system showed that RGDS and a5b1 ab injection decreased chondrocyte proliferation, differentiation, and apoptosis in enchondral ossification, and suggested that the integrin a5b1-mediated ECM signal through the RGD sequence is involved in embryonic enchondral ossification.

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Section: Discussionsupporting

confidence: 52%

Section: Discussionsupporting

confidence: 49%

In Vivo Analysis of Arg-Gly-Asp Sequence/Integrin α5β1-Mediated Signal Involvement in Embryonic Enchondral Ossification by Exo Utero Development System

Inoue

Hashimoto

Matsumoto

et al. 2013

Journal of Bone and Mineral Research

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“…The epiphyseal growth plate of the bm/bm mice lacks the organization seen in the control mice at all ages. This finding is in agreement with other reports 9,32 . Vanky et al 9 found that the chondrocytes in the proliferating zone are in the G 0 phase of the cell cycle which would result in a decrease in the number of hypertrophic cells.…”

Section: Discussionsupporting

confidence: 94%

Degenerative knee joint disease in mice lacking 3′-phosphoadenosine 5′-phosphosulfate synthetase 2 (Papss2) activity: a putative model of human PAPSS2 deficiency-associated arthrosis

Ford-Hutchinson

Ali

Seerattan

et al. 2005

Osteoarthritis and Cartilage

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“…Wikstrom et al. (11) carried out a morphologic study on the epiphyseal growth zone, and Wezeman and Bollnow (12) studied tibial growth plate articular cartilage in other bm mice (C57BI/6J × C3He). Kajii et al.…”

Section: Introductionmentioning

confidence: 99%

Histological study of the nasal septal cartilage in BALB/c‐bm/bm mouse which spontaneously induces malocclusion

Takahashi

Kajii

Tsukamoto

et al. 2012

Orthod Craniofacial Res

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Morphological studies of the epiphyseal growth zone in the brachymorphic (bm/bm) mouse

Cited by 19 publications

References 14 publications

In Vivo Analysis of Arg-Gly-Asp Sequence/Integrin α5β1-Mediated Signal Involvement in Embryonic Enchondral Ossification by Exo Utero Development System

In Vivo Analysis of Arg-Gly-Asp Sequence/Integrin α5β1-Mediated Signal Involvement in Embryonic Enchondral Ossification by Exo Utero Development System

Degenerative knee joint disease in mice lacking 3′-phosphoadenosine 5′-phosphosulfate synthetase 2 (Papss2) activity: a putative model of human PAPSS2 deficiency-associated arthrosis

Histological study of the nasal septal cartilage in BALB/c‐bm/bm mouse which spontaneously induces malocclusion

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