Morphological Structure and Genetic Mapping of New Leaf-Color Mutant Gene in Rice (Oryza sativa)

Li, Yuhong; Wang, Baohe; Dai, Z L; Li, Aihong; Liu, Guangqing; Zuo, Shimin; Zhang, Hongxi; X, Pan

doi:10.1016/s1672-6308(12)60025-0

Cited by 11 publications

(8 citation statements)

References 16 publications

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“…Many leaf variegation mutants have defects in chloroplast development. For example, chloroplasts in the fgl mutant display defective thylakoid stacking and have several plastoglobules (Sakuraba et al 2013 ), and chloroplasts in the yellow-green leaf 2 ( ygl2 ) and the naturally occurring Baihuaidio 7 mutants exhibit fewer lamellar structures (Chen et al 2013 ; Li et al 2012 ). To determine whether the leaves in the z3 mutant show defects in chloroplast structure, we performed ultrastructural analysis of the chloroplasts in the leaves of the wild type and the z3 mutant.…”

Section: Resultsmentioning

confidence: 99%

The rice zebra3 (z3) mutation disrupts citrate distribution and produces transverse dark-green/green variegation in mature leaves

Kim

Kwon

Song

et al. 2018

Rice

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BackgroundRice zebra mutants are leaf variegation mutants that exhibit transverse sectors of green/yellow or green/white in developing or mature leaves. In most cases, leaf variegation is caused by defects in chloroplast biogenesis pathways, leading to an accumulation of reactive oxygen species in a transverse pattern in the leaves. Here, we examine a new type of leaf variegation mutant in rice, zebra3 (z3), which exhibits transverse dark-green/green sectors in mature leaves and lacks the typical yellow or white sectors.ResultsMap-based cloning revealed that the Z3 locus encodes a putative citrate transporter that belongs to the citrate-metal hydrogen symport (CitMHS) family. CitMHS family members have been extensively studied in bacteria and function as secondary transporters that can transport metal-citrate complexes, but whether CitMHS family transporters exist in eukaryotes remains unknown. To investigate whether Z3 acts as a citrate transporter in rice, we measured citrate levels in wild-type leaves and in the dark-green and green sectors of the leaves of z3 mutants. The results showed that citrates accumulated to high levels in the dark-green sectors of z3 mutant leaves, but not in the green sectors as compared with the wild-type leaves.ConclusionsThese results suggest that leaf variegation in the z3 mutant is caused by an unbalanced accumulation of citrate in a transverse pattern in the leaves. Taking these results together, we propose that Z3 plays an important role in citrate transport and distribution during leaf development and is a possible candidate for a CitMHS family member in plants.Electronic supplementary materialThe online version of this article (10.1186/s12284-017-0196-8) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

The rice zebra3 (z3) mutation disrupts citrate distribution and produces transverse dark-green/green variegation in mature leaves

Kim

Kwon

Song

et al. 2018

Rice

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“…In this study, we mainly studied chlorophyll and carotenoids. Many chlorophyll-deficient mutants were also found in rice [30] and Arabidopsis thaliana [31], and the lack of chlorophyll resulted in a physiological change in leaf color. Our results showed that SPAD value, Chl a, Chl b and Chl a + b contents in 'SO' were all higher than those in 'ZH', while the carotenoid content in 'ZH' was significantly higher than that in 'SO', indicating that the combination of chlorophyll reduction and carotenoid increase might induce yellow leaf coloration of 'ZH'.…”

Section: Discussionmentioning

confidence: 99%

Physiological and Anatomical Differences and Differentially Expressed Genes Reveal Yellow Leaf Coloration in Shumard Oak

Dong

Huang

Chen

et al. 2020

Plants

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Shumard oak (Quercus shumardii Buckley) is a traditional foliage plant, but little is known about its regulatory mechanism of yellow leaf coloration. Here, the yellow leaf variety of Q. shumardii named ‘Zhongshan Hongjincai’ (identified as ‘ZH’ throughout this work) and a green leaf variety named ‘Shumard oak No. 23’ (identified as ‘SO’ throughout this work) were compared. ‘ZH’ had lower chlorophyll content and higher carotenoid content; photosynthetic characteristics and chlorophyll fluorescence parameters were also lower. Moreover, the mesophyll cells of ‘ZH’ showed reduced number of chloroplasts and some structural damage. In addition, transcriptomic analysis identified 39,962 differentially expressed genes, and their expression levels were randomly verified. Expressions of chlorophyll biosynthesis-related glumly-tRNA reductase gene and Mg-chelatase gene were decreased, while pheophorbide a oxygenase gene associated with chlorophyll degradation was up-regulated in ‘ZH’. Simultaneously, carotenoid isomerase gene, z-carotene desaturase gene, violaxanthin de-epoxidase gene and zeaxanthin epoxidase gene involved in carotenoid biosynthesis were up-regulated in ‘ZH’. These gene expression changes were accompanied by decreased chlorophyll content and enhanced carotenoid accumulation in ‘ZH’. Consequently, changes in the ratio of carotenoids to chlorophyll could be driving the yellow leaf coloration in Q. shumardii.

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“…Leaf color mutation in plants is common and there is a rich variation in nature. The mutated genes not only impact the biosynthesis and degradation of chlorophyll by direct or indirect way, but also affect the growth and development of plants [31]. In this study, for the first time we analyzed the genetic, cytologic, and proteomic data of the leaf color mutant Siyl-1 .…”

Section: Discussionmentioning

confidence: 99%

Cytologic, Genetic, and Proteomic Analysis of a Yellow Leaf Mutant of Sesame (Sesamum indicumL.),Siyl-1

Gao

Sang

Chen

et al. 2018

Preprint

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11Leaf color mutation in sesame always affects the growth and development of plantlets, and their 12 yield. To clarify the mechanisms underlying leaf color regulation in sesame, we analyzed a 13 yellow-green leaf mutant. Genetic analysis of the mutant selfing revealed 3 phenotypes-YY, 14 light-yellow (lethal); Yy, yellow-green; and yy, normal green-controlled by an incompletely 15 dominant nuclear gene, Siyl-1. In YY and Yy, the number and morphological structure of the 16 chloroplast changed evidently, with disordered inner matter, and significantly decreased 17 chlorophyll content. To explore the regulation mechanism of leaf color mutation, the proteins 18 expressed among YY, Yy, and yy were analyzed. All 98 differentially expressed proteins (DEPs) 19 were classified into 5 functional groups, in which photosynthesis and energy metabolism 20 (82.7%) occupied a dominant position. Our findings provide the basis for further molecular 21 mechanism and biochemical effect analysis of yellow leaf mutants in plants. 22 24which also contain numerous beneficial minerals, antioxidants, and multi-vitamins [1]. 25Compared with other oilseed crops, sesame is still a low yield crop with low harvest index. 26The key research objectives in sesame are to increase the photosynthesis efficiency and the 27 yield per square area. 28Leaf color is an important trait related with the chlorophyll content, and always affects the 29 photosynthesis efficiency and the final productivity [2]. In plants, the chloroplast structure 30 always alters the composition and content of photosynthetic pigments. Several studies on the 31 leaf color mutants have uncovered a wide range of leaf color mutation types [3]. Most 32 mutagenesis of leaf color were related to the structure and function of the chloroplast [4], 33 chlorophyll biosynthesis and degradation mechanisms [5], photosynthesis [6], chloroplast 34 developmental characteristics [7], genetics [8], and molecular mechanisms [9]. Furthermore, 35some mutants were used for crop breeding to higher biomass, quality, and/or other specific 36 characteristics [10]. There is a rich diversity of leaf color mutation types in plants [11][12][13][14]. 37Leaf color mutants were divided into five types according to the color classification albino, 38 yellowing, light-green, stripe, spot, etc [15, 16]. Falbel [12] divided the chlorophyll mutant 39 into two categories: (1) mutants that lack chlorophyll b, such as arabidopsis mutants [17], and 40(2) mutants with reduced synthesis of total chlorophyll and chlorophyll b; currently most 41 mutants belong to the latter category. Leaf color characteristics controlled by both nuclear 42 inheritance and cytoplasmic inheritance, may be a quantitative or a quality trait [18]. Most of 43 the leaf color variations are caused by nuclear gene mutation inducing a type of chlorophyll 44 deficiency in higher plants. The leaf color variations are mostly monogenic mutation, while a 45 handful of them are polygenic mutation. Among these mutations, the recessive mutations are 4...

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Morphological Structure and Genetic Mapping of New Leaf-Color Mutant Gene in Rice (Oryza sativa)

Cited by 11 publications

References 16 publications

The rice zebra3 (z3) mutation disrupts citrate distribution and produces transverse dark-green/green variegation in mature leaves

The rice zebra3 (z3) mutation disrupts citrate distribution and produces transverse dark-green/green variegation in mature leaves

Physiological and Anatomical Differences and Differentially Expressed Genes Reveal Yellow Leaf Coloration in Shumard Oak

Cytologic, Genetic, and Proteomic Analysis of a Yellow Leaf Mutant of Sesame (Sesamum indicumL.),Siyl-1

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