Morphological polymorphysm of hepatocytes and regeneration in survivors from fulminant hepatitis and acute hepatitis with bridging hepatic necrosis.

守一, 関; 重義, 針原; 忍, 斉藤; 進, 塩見; 靖紘, 溝口; 哲夫, 黒木; 祐夫, 山本; 丈之, 門奈

doi:10.2957/kanzo.27.1056

Cited by 3 publications

(1 citation statement)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In confluent necrosis of AVH, small hepatocytes with basophilic cytoplasm next to the confluent necrosis were important in the hepatic regeneration. When dropping-out of hepatocytes was extensive, these small, relatively immature hepatocytes probably entered the mitotic cell cycle repeatedly, increasing in number and repairing the confluent necrotic areas (18,33). In addition to hepatocytes, some Kupffer cells or other macrophages and some lymphocytes were stained for DNA-PA in the confluent necrotic areas.…”

Section: Discussionmentioning

confidence: 99%

Detection of proliferating liver cells in various diseases by a monoclonal antibody against DNA polymerase-α: With special reference to the relationship between hepatocytes and sinusoidal cells

et al. 1991

Self Cite

View full text Add to dashboard Cite

Proliferating cells in liver specimens from patients with various diseases were detected by use of a monoclonal antibody against human DNA polymerase-alpha, which is present in the nuclei of cells in the G1, S, M and G2 phases of the mitotic cell cycle and absent in the G0 phase, to clarify the kinetics and morphological characteristics of these cells. This monoclonal antibody was supernatant derived from clone CL22-2-42B, and the peroxidase antiperoxidase method was used. Not only epithelial cells (hepatocytes, biliary epithelial cells and hepatocellular carcinoma cells) but also nonepithelial cells (Kupffer cells and other macrophages, endothelial cells, fat-storing cells, lymphocytes and fibroblasts) were stained for DNA polymerase-alpha. In acute viral hepatitis with confluent necrosis, small hepatocytes with basophilic cytoplasm next to the necrosis accounted for most of the proliferating cells. In these areas, Kupffer cells and other macrophages and lymphocytes had often proliferated. Hepatocellular carcinoma cells were frequently stained for DNA polymerase-alpha, in addition to endothelial cells, macrophages and lymphocytes. These nonepithelial cells were stained more frequently in specimens with many stained carcinoma cells than in those with only a few cells stained. In fibrotic areas, fibroblasts were often stained for this enzyme. In proliferating bile ducts, both small epithelial cells and large mature cells were stained. The differences between stained and nonstained cells that were not hepatocytes could not be defined by their ultrastructural characteristics. From these findings, it seemed possible that sinusoidal cells, especially Kupffer cells and other macrophages, might be much involved in hepatocytic proliferation during regeneration of the liver and also in the occurrence of malignant tumors.

show abstract

Section: Discussionmentioning

confidence: 99%

Detection of proliferating liver cells in various diseases by a monoclonal antibody against DNA polymerase-α: With special reference to the relationship between hepatocytes and sinusoidal cells

et al. 1991

Self Cite

View full text Add to dashboard Cite

show abstract

Structural and functional aspects of regeneration of human liver

Schaffner

1991

Digest Dis Sci

View full text Add to dashboard Cite

Regeneration of human liver was long suspected to occur. It was proven in animals 100 years ago but could not be demonstrated in man until liver biopsy and modern hepatic tests became available. Structural changes in the regenerating liver mainly concern the arrangement of liver cell plates and the size and appearance of hepatocytic nuclei. A return to normalcy in test results depends on the factors responsible for regeneration since various test results change at different rates. Mass, estimated by imaging procedures, is restored parallel with the return of function. Shape is not restored but the pressure of neighboring organs and structures molds the growing remnant so that it almost resembles the original. Factors regulating regeneration in man are beginning to be recognized as they have been in animals. The hope is that regeneration can be accelerated or that cells can be transplanted to replace those lost.

show abstract

Proliferating cells evaluated histologically with a monoclonal antibody against DNA polymerase .ALPHA. in various liver diseases.

浩樹¹,

守一²,

篤³

et al. 1990

Acta hepatologica Japonica

View full text Add to dashboard Cite

Morphological polymorphysm of hepatocytes and regeneration in survivors from fulminant hepatitis and acute hepatitis with bridging hepatic necrosis.

Cited by 3 publications

References 9 publications

Detection of proliferating liver cells in various diseases by a monoclonal antibody against DNA polymerase-α: With special reference to the relationship between hepatocytes and sinusoidal cells

Detection of proliferating liver cells in various diseases by a monoclonal antibody against DNA polymerase-α: With special reference to the relationship between hepatocytes and sinusoidal cells

Structural and functional aspects of regeneration of human liver

Proliferating cells evaluated histologically with a monoclonal antibody against DNA polymerase .ALPHA. in various liver diseases.

Contact Info

Product

Resources

About