Morphological peculiarities of the DNA-protein complexes in starved Escherichia coli cells

Лойко, Н. Г.; Danilova, Yana; Moiseenko, Andrey; Kovalenko, Vladislav; Терешкина, К. Б.; Tutukina, Maria N.; Эль-Регистан, Г. И.; Sokolova, Olga; Krupyanskiǐ, Yu. F.

doi:10.1371/journal.pone.0231562

Cited by 33 publications

(30 citation statements)

References 48 publications

(114 reference statements)

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“…A particular feature of a population of ATX3-Q24 induced cells is the presence of condensed DNA ( Figure 8 B, single asterisk). Interestingly, this kind of DNA structure was observed in bacteria under different stress conditions, like nutritional stress [ 51 , 52 , 53 ]. Cytoplasmic IBs ( Figure 8 G, double asterisk) and production of extra-cytoplasmic vesicles (arrows) were visible in both over-expressing strains, as a response of intracellular protein accumulation.…”

Section: Resultsmentioning

confidence: 99%

Pathological ATX3 Expression Induces Cell Perturbations in E. coli as Revealed by Biochemical and Biophysical Investigations

Ami

Sciandrone

Mereghetti³

et al. 2021

IJMS

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Amyloid aggregation of human ataxin-3 (ATX3) is responsible for spinocerebellar ataxia type 3, which belongs to the class of polyglutamine neurodegenerative disorders. It is widely accepted that the formation of toxic oligomeric species is primarily involved in the onset of the disease. For this reason, to understand the mechanisms underlying toxicity, we expressed both a physiological (ATX3-Q24) and a pathological ATX3 variant (ATX3-Q55) in a simplified cellular model, Escherichia coli. It has been observed that ATX3-Q55 expression induces a higher reduction of the cell growth compared to ATX3-Q24, due to the bacteriostatic effect of the toxic oligomeric species. Furthermore, the Fourier transform infrared microspectroscopy investigation, supported by multivariate analysis, made it possible to monitor protein aggregation and the induced cell perturbations in intact cells. In particular, it has been found that the toxic oligomeric species associated with the expression of ATX3-Q55 are responsible for the main spectral changes, ascribable mainly to the cell envelope modifications. A structural alteration of the membrane detected through electron microscopy analysis in the strain expressing the pathological form supports the spectroscopic results.

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Section: Resultsmentioning

confidence: 99%

Pathological ATX3 Expression Induces Cell Perturbations in E. coli as Revealed by Biochemical and Biophysical Investigations

Ami

Sciandrone

Mereghetti³

et al. 2021

IJMS

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“…4, Table 2). The remaining cells develop into dormant forms, which differ significantly from the growing cells in their structural organization [28].…”

Section: Dormant Cells Starvation Stress Structural Response To Stressmentioning

confidence: 99%

“…Experiments on synchrotron radiation scattering measurements were performed on the samples containing E. coli cells (BL21-Gold (DE3) strain) transformed with the pET-Dps plasmid and subjected to induction of increased expression of the Dps protein [28][29][30]. For the cell population under starvation stress, diffraction patterns were recorded (insert, Fig.…”

Section: Sin D λ = θmentioning

confidence: 99%

“…The sample preparation procedure is described in the original papers [28][29][30]43]. the fungus Umbelopsis ramanniana, apparently, also form ordered (nanocrystalline) structures, as evidenced by the zones of increased scattering intensity with lattice constants from 27-28 Å to 55 Å.…”

Section: Table 2 Number Of Surviving Cells Under Starvation Stressmentioning

confidence: 99%

“…Of particular interest is the third type of ordered structure, which was revealed for the first time in the dormant E. coli cells [28], namely, a folded nucleosome-like structure. In all the populations under study (except for the Dps-null mutant) with and without excess Dps production, the cytoplasm of 5 to 25% of cells is filled with many spherical structures (Figs.…”

Section: In Vitro Dna Condensation Study Of Model Systemsmentioning

confidence: 99%

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Architecture of Nucleoid in the Dormant Cells of Escherichia coli

Krupyanskiǐ

2021

Russ. J. Phys. Chem. B

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The review presents the results of the experimental studies of the structure of condensed DNA in the nucleoid of the dormant cells of Escherichia coli bacteria that appear in response to starvation stress. The conformation of DNA was studied by synchrotron radiation diffraction and transmission electron microscopy. The series of diffraction experiments revealed a periodic ordered organization (most likely, nanocrystalline) of DNA with DNA-associated proteins in all objects under study subjected to starvation stress: bacteria E. coli, spores and bacteria Bacillus Cereus, and filamentous fungi Umbelopsis ramanniana. A transmission electron microscopy study gave fine visual information about the type of DNA condensation in the nucleoid of the E. coli bacterium. The TEM study made it possible to find the intracellular nanocrystalline, liquid crystalline, and folded nucleosome-like DNA structures. The proportion of specific structure depends on the strain and cultivation conditions. The folded nucleosome-like structure was observed and described in the authors' original studies for the first time. The electron microscopy studies do not allow direct visualization of DNA; therefore, the DNA conformation is hypothetical. To obtain a clear picture of the DNA structure in intracellular crystals, the crystals were studied in vitro by X-ray macromolecular crystallography and electron microscopy. In contrast to diffraction experiments, the electron microscopy studies made it possible to see DNA traces in thin (2D) DNA-Dps crystals. A hypothetical model of DNA conformation in intracellular nanocrystals was proposed. The results of experiments made it possible to visualize the structures of the lower hierarchical tier of DNA compaction in the nucleoid of dormant cells.

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Application of Special Electron Microscopy Techniques to the Study of DNA – Protein Complexes in E. coli Cells

Moiseenko

Лойко

Sokolova

et al. 2022

Methods in Molecular Biology

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Morphological peculiarities of the DNA-protein complexes in starved Escherichia coli cells

Cited by 33 publications

References 48 publications

Pathological ATX3 Expression Induces Cell Perturbations in E. coli as Revealed by Biochemical and Biophysical Investigations

Pathological ATX3 Expression Induces Cell Perturbations in E. coli as Revealed by Biochemical and Biophysical Investigations

Architecture of Nucleoid in the Dormant Cells of Escherichia coli

Application of Special Electron Microscopy Techniques to the Study of DNA – Protein Complexes in E. coli Cells

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