Morphological Observation on Cell Death and Phagocytosis Induced by Ultraviolet Irradiation in a Cultured Human Lens Epithelial Cell Line

Shui, Ying-Bo; Sekiya, Hiroshi; Pan, Jiehong; Hata, Ikuho; Kojima, Masami; Yamada, Yoshihisa; Hirai, Kei-Ichi; Takahashia, Nobuo; Sasaki, Kohsuke

doi:10.1006/exer.2000.0917

Cited by 29 publications

(19 citation statements)

References 23 publications

(26 reference statements)

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“…We therefore hypothesized that lens epithelial cells themselves could function as endogenous phagocytes of the lens that could remove apoptotic debris generated by lens exposure to UV light and other environmental insults. Consistently, other investigators have detected apoptosis-like material contained within lens epithelial cell vesicles in isolated lens cells treated with UV light (40) and in the lenses of rodents treated with UV light (21,30).…”

Section: Discussionsupporting

confidence: 78%

“…Early studies identified the presence of vesicles containing what was described as apoptosis-like material in UV-treated cultured lens epithelial cells (40) and in the intact lenses of UV-treated rodents (21,31), suggesting the possibility that lens cells could act as phagocytes to remove and detoxify the cell debris in the apoptotic lens caused by UV light exposure. Similar vesicles have also been found in the lens epithelium of UV light-treated rabbit lenses (24) and human age-related cataract lenses (32).…”

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confidence: 99%

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Integrin αVβ5-mediated Removal of Apoptotic Cell Debris by the Eye Lens and Its Inhibition by UV Light Exposure

Chauss

Brennan

Бакина

et al. 2015

Journal of Biological Chemistry

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Accumulation of apoptotic material is toxic and associated with cataract and other disease states. Identification of mechanisms that prevent accumulation of apoptotic debris is important for establishing the etiology of these diseases. The ocular lens is routinely assaulted by UV light that causes lens cell apoptosis and is associated with cataract formation. To date, no molecular mechanism for removal of toxic apoptotic debris has been identified in the lens. Vesicular debris within lens cells exposed to UV light has been observed raising speculation that lens cells themselves could act as phagocytes to remove toxic apoptotic debris. However, phagocytosis has not been confirmed as a function of the intact eye lens, and no mechanism for lens phagocytosis has been established. Here, we demonstrate that the eye lens is capable of phagocytizing extracellular lens cell debris. Using high throughput RNA sequencing and bioinformatics analysis, we establish that lens epithelial cells express members of the integrin ␣V␤5-mediated phagocytosis pathway and that internalized cell debris co-localizes with ␣V␤5 and with RAB7 and Rab-interacting lysosomal protein that are required for phagosome maturation and fusion with lysosomes. We demonstrate that the ␣V␤5 receptor is required for lens epithelial cell phagocytosis and that UV light treatment of lens epithelial cells results in damage to the ␣V␤5 receptor with concomitant loss of phagocytosis. These data suggest that loss of ␣V␤5-mediated phagocytosis by the eye lens could result in accumulation of toxic cell debris that could contribute to UV light-induced cataract formation.

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Section: Discussionsupporting

confidence: 78%

mentioning

confidence: 99%

Integrin αVβ5-mediated Removal of Apoptotic Cell Debris by the Eye Lens and Its Inhibition by UV Light Exposure

Chauss

Brennan

Бакина

et al. 2015

Journal of Biological Chemistry

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“…Among the active cells, LECs were of interest because of the possible relation between controlled cell death (apoptosis) and the occurrence of cataractous changes. 15,16 These results will be presented in a future article.…”

Section: Introductionmentioning

confidence: 84%

Nonresonant Raman imaging of protein distribution in single human cells

et al. 2002

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A confocal Raman microscope is used to study the protein distribution inside biological cells. It is shown that high quality Raman imaging of the protein distribution can be obtained using confocal nonresonant Raman imaging ( exc ϭ 647.1 nm). The results are shown for two different human cell types. Perpheral blood lymphocytes are used as an example of the fully maturated cells with a low level of nuclear transcription. Human eye lens epithelial cells are used as an example of cells with a high level of nuclear activity. The protein distribution in both cell types is completely different. The nuclear distribution of the protein largely varies in the peripheral blood lymphocyte cells, while proteins are more homogenously distributed over the nuclear space in the eye lens epithelial cells. The imaging time is ϳ20 min for a field of view of 10 ϫ 10 m 2 . The size of the sampling volume is 1.4 fL using a full width at halfmaximum criterion along the z axis and a 1/e 2 criterion in the xy plane. The results presented here indicate that Raman imaging is particularly of interest in the study of cellular processes, like phagocytosis, apoptosis, chromatin compaction, and cellular differentiation, which are accompanied by relatively large-scale redistributions of the materials.

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“…The eyeballs were dissected and fixed in ethanol/acetic acid (3:1 v/v) for at least 24 h. Through an anterior approach, the lens was removed. The lens epithelium was peeled off under a dissecting microscope, stained with hematoxylin, propidium iodide, and TUNEL technique [13] and observed under a light microscope or a fluorescence microscope [9]. The corneal specimens were processed for light microscopic evaluation after hematoxylin and eosin staining.…”

Section: Histological Studymentioning

confidence: 99%

Ultraviolet B-Induced Corneal and Lens Damage in Guinea Pigs on Low-Ascorbic Acid Diet

Wu¹,

Kojima²,

Shui³

et al. 2004

Ophthalmic Res

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To evaluate ocular morphological response to ultraviolet B (UV-B) exposure in guinea pigs fed with normal and low-ascorbic acid (ASA) diets, 24 guinea pigs were randomly divided into UVR (normal diet + UV-B), LVC (low-ASA diet + UV-B) and CTL (normal diet, without UV-B) groups. The irradiated animals were exposed to 8.2 J/cm² of UV-B over 29 weeks. Changes in the cornea and lens were examined using a Scheimpflug camera, specular microscope and histological studies. ASA contents in the plasma, aqueous humor and lens sections were measured by high-performance liquid chromatography. The results showed low contents of ASA in the plasma, aqueous humor and lens sections in LVC. Both irradiated groups displayed corneal epithelial edema, neovascularization in stroma, increase in corneal thickness, decrease in endothelial density, and lens epithelial damage. No significant morphological differences in cornea and lens were found between guinea pigs fed with low-ASA or normal diet.

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Morphological Observation on Cell Death and Phagocytosis Induced by Ultraviolet Irradiation in a Cultured Human Lens Epithelial Cell Line

Cited by 29 publications

References 23 publications

Integrin αVβ5-mediated Removal of Apoptotic Cell Debris by the Eye Lens and Its Inhibition by UV Light Exposure

Integrin αVβ5-mediated Removal of Apoptotic Cell Debris by the Eye Lens and Its Inhibition by UV Light Exposure

Nonresonant Raman imaging of protein distribution in single human cells

Ultraviolet B-Induced Corneal and Lens Damage in Guinea Pigs on Low-Ascorbic Acid Diet

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