Morphological mutants of Gibberella fujikuroi for enhanced production of gibberellic acid

Lale, Geetanjali; Jogdand, V. V.; Gadre, R. V.

doi:10.1111/j.1365-2672.2005.02754.x

Cited by 16 publications

(15 citation statements)

References 18 publications

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“…In our previous study [20], we reported on the use of morphological mutants that have short mycelial lengths in liquid cultures, which led to better oxygen transfer and increased production of GA 3 . Mor-189, used in the present investigation, is also a morphological mutant, similar to the mutants described earlier, and has the advantages of a low Fig.…”

Section: Discussionmentioning

confidence: 99%

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Enhanced production of gibberellin A4 (GA4) by a mutant of Gibberella fujikuroi in wheat gluten medium

Lale¹,

Gadre²

2009

J Ind Microbiol Biotechnol

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Mutants of Gibberella fujikuroi with different colony characteristics, morphology and pigmentation were generated by exposure to UV radiation. A mutant, Mor-189, was selected based on its short filament length, relatively high gibberellin A(4) (GA(4)) and gibberellin A(3) (GA(3)) production, as well as its lack of pigmentation. Production of GA(4) by Mor-189 was studied using different inorganic and organic nitrogen sources, carbon sources and by maintaining the pH of the fermentation medium using calcium carbonate. Analysis of GA(4) and GA(3) was done by reversed-phase high-performance liquid chromatography and LC-MS. The mutants of G. fujikuroi produced more GA(4) when the pH of the medium was maintained above 5. During shake flask studies, the mutant Mor-189 produced 210 mg l(-1) GA(4) in media containing wheat gluten as the nitrogen source and glucose as the carbon source. Fed-batch fermentation in a 14 l agitated fermenter was performed to evaluate the applicability of the mutant Mor-189 for the production of GA(4). In 7-day fed-batch fermentation, 600 mg l(-1) GA(4) were obtained in the culture filtrate. The concentration of GA(4) and GA(3) combined was 713 mg l(-1), of which GA(4) accounted for 84% of the total gibberellin. These values are substantially higher than those published previously. The present study indicated that, along with maintenance of pH and controlled glucose feeding, the use of wheat gluten as the sole nitrogen source considerably enhances GA(4) production by the mutant Mor-189.

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Section: Discussionmentioning

confidence: 99%

“…An exposure time of seven seconds was found to be suitable for achieving a 95% kill rate and was used in subsequent mutagenesis experiments. In our previous study [20] we reported on mutants that were selected based on their short filament length mycelia in the fermentation broth.…”

Section: Mutagenesis and Screeningmentioning

confidence: 99%

Enhanced production of gibberellin A4 (GA4) by a mutant of Gibberella fujikuroi in wheat gluten medium

Lale¹,

Gadre²

2009

J Ind Microbiol Biotechnol

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“…For example, Lale et al obtained mutant strains with the shorter mycelium length which greatly improved the level of dissolved oxygen in the fermentation process, the yield of GA3 was nearly 2 times higher than the original strain. 22 In addition, the novel fermentation methods, such as cell immobilization and extractive fermentation techniques, were also successfully applied in the microbial production of GAs. Lu and Xie immobilized G. fujikuroi on the surface of the cellulose carrier which could continuously and efficiently produce GA3 in 84 days, the ultimate GA3 titer reached 0.21 g/L.…”

Section: Introductionmentioning

confidence: 99%

Microbial production of plant hormones: Opportunities and challenges

et al. 2016

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Plant hormones are a class of organic substances which are synthesized during the plant metabolism. They have obvious physiological effect on plant growth at very low concentrations. Generally, plant hormones are mainly divided into 5 categories: auxins, cytokinins, ethylene, gibberellins (GAs) and abscisic acid (ABA). With the deepening of research, some novel plant hormones such as brassinosteroid and salicylates have been found and identified. The plant hormone products are mainly obtained through plant extraction, chemical synthesis as well as microbial fermentation. However, the extremely low yield in plants and relatively complex chemical structure limit the development of the former 2 approaches. Therefore, more attention has been paid into the microbial fermentative production. In this commentary, the developments and technological achievements of the 2 important plant hormones (GAs and ABA) have been discussed. The discovery, producing strains, fermentation technologies, and their accumulation mechanisms are first introduced. Furthermore, progresses in the industrial mass scale production are discussed. Finally, guidelines for future studies for GAs and ABA production are proposed in light of the current progress, challenges and trends in the field. With the widespread use of plant hormones in agriculture, we believe that the microbial production of plant hormones will have a bright future.

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“…This GA yield exceeded most amounts reported by other researchers. Yields of 0.7 g/L using G. fujikuroi (Lale et al, 2006); 1.82 g/L by F. moniliforme (Kobomoje et al, 2013); 2.8g/L by F. moniliforme (Pastrana et al, 1993); 11.3 g/L by F. moniliforme (Bilkay et al, 2010) and 460.06 mg/L by G. fujikuroi were reported by Cuali-Alvarez et al (2011). However, higher yields of 15 g/L and 32.8 g/L have been reported by Rangaswamy (2012) and Omojasola and Benu (2016) on Jatropha seedcake using F. moniliforme and A. terreus respectively.…”

Section: Resultsmentioning

confidence: 85%

Gibberellic Acid Production by Fusarium moniliforme and Aspergillus niger Using Submerged Fermentation of Banana Peel

Omojasola

Adejoro

2018

Not Sci Biol

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The present study aimed to produce gibberellic acid through fermentation using banana (Musa sapientum) peel waste as substrate. Banana peel, a domestic and industrial waste, constitutes a potential source of cheap fermentable substrate for the production of other value-added products. Fusarium moniliforme ATCC 10052 and Aspergillus niger CBS 513.88 were used as fermenting organisms. The substrate was dried, ground and its proximate composition determined. The powdered substrate was added to a modified CzapekDox broth (a semisynthetic medium), with Carboxyl methylcellulose (CMC) as control. The fermentation conditions were: pH 5.5; inoculum size 1% (5 × 10 5 spores/mL F. moniliforme) (2 × 10 6 spores/mL A. niger); substrate concentration 2 g; temperature 25 ± 2 o C; fermentation time 7 days. The fermentation was optimized by varying pH, inoculum size, substrate concentration and fermentation time. The extracted GA was subjected to infra-red spectroscopy using FT-IR. The parameters which gave the highest GA yields were thereafter combined in a single fermentation. The results of proximate analysis of banana peel substrate revealed 8.65% moisture, 9.54% protein, 5.40% lipids, 11.45% ash, 22.34% crude fibre, and 42.62% carbohydrate. The GA yields of 13.55 g/L and 12.44 g/L were produced from the banana peel substrate and 3.62 and 2.61 g/L from the CMC control by F. moniliforme and A. niger respectively. Under optimized conditions, F. moniliforme produced 17.48 g/L GA, while A. niger produced 13.50 g/L. Extracted GA was similar to standard GA sample and the present results support the potential use of banana peel for fermentative GA production.

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Morphological mutants of Gibberella fujikuroi for enhanced production of gibberellic acid

Cited by 16 publications

References 18 publications

Enhanced production of gibberellin A4 (GA4) by a mutant of Gibberella fujikuroi in wheat gluten medium

Enhanced production of gibberellin A4 (GA4) by a mutant of Gibberella fujikuroi in wheat gluten medium

Microbial production of plant hormones: Opportunities and challenges

Gibberellic Acid Production by Fusarium moniliforme and Aspergillus niger Using Submerged Fermentation of Banana Peel

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