Morphological localization of apolipoproteins and their mRNA by immunocytochemistry and in situ nucleic acid hybridization

Lin, Chin‐Tarng

doi:10.1016/0076-6879(86)29077-1

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1986

1992

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Cited by 2 publications

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“…The procedure for cell fixation and hybridization was performed according to our previous method (6,7), with some modifications. The CaSki cells were cytospun (700 rpm, 10 min) on alcohol-acid-cleaned glass slides which had been pre-coated with 2 % 3-aminopropyl uiethoxysilane, fixed in 3% parafor- 7.0).…”

Section: Methodsmentioning

confidence: 99%

Localization of HPV-16 DNA sequence in CaSki cells by electron microscopic hybridocytochemistry.

Lin

Chen²,

How³

et al. 1992

J Histochem Cytochem.

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We investigated the HPV-16 DNA sequence in the CaSki cervical carcinoma cell line by electron microscopic hybridocytochemistry using biotinylated HPV-16/18 probes. At the light microscopic level, reaction product of hybridized HPV-16 DNA sequence was not seen in the cytoplasm but appeared as spots or rods randomly distributed in the nuclei. By electron miaoscopy, reaction product was seen aggregated in several regions in the nuclei. Most of the stained areas did not reveal particular architecture but showed part of the chromatin structure. In other nuclei, reaction product was observed to be associated with strings of loop-like structure, and some stained loops were seen to be connected directly

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Section: Methodsmentioning

confidence: 99%