Morphological Interactions between Glial Fibrillary Acidic Protein (GFAP)-like Immunoreactive Elements and Lutenizing Hormone Releasing Hormone (LHRH)-like Immunoreactive Nerve Endings in the Median Eminence of the Rat. Double Labeling Immunoelectron Microscopic Study.

Tamada, Yoshitaka; Hayashi, Seiji; Munekawa, Katsuhiko; Tanaka, Masaki; Ikeda, Teru; Inoue, Kenzo; Ibata, Yasuhiko

doi:10.1267/ahc.30.517

Cited by 3 publications

(4 citation statements)

References 28 publications

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“…Immunoelectron microscopic findings for the young adult group were previously reported by Tamada et al [26].…”

Section: Immunoelectron Microscopysupporting

confidence: 66%

“…It is thought that these glial cells are involved in the regulation of the release of the releasing/inhibiting (R/I) hormones in the ME [11,13,[15][16][17]26].…”

Section: Introductionmentioning

confidence: 99%

“…Astrocytes are immunoreactive to GFAP and immunonegative to VIM [4]. Astrocytic cell bodies are mainly distributed in the subependymal layer and their processes have been observed throughout the ME [26].…”

Section: Introductionmentioning

confidence: 99%

“…In the ME, it has already been clarified morphologically that the GFAP-immunoreactive processes are in close contact with nerve endings such as the synapses, and a specific contact pattern is also exhibited between these processes and the basement membrane of the capillaries in the perivascular region [26]. The microvasculature of the ME in the anterior hypothalamo-hypophyseal system (HHS) was investigated in the rat by Murakami et al [18].…”

Section: Introductionmentioning

confidence: 99%

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Age-related Changes in Astrocytes and Microvasculature in the Median Eminence of the Rat

Ike

Tamada

Uemura

et al. 2004

Acta Histochem. Cytochem

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Age-related changes in astrocytes, tanycytes and the microvasculature were investigated in the median eminence (ME) of the anterior hypothalamo-hypophyseal system of 2-and 24-month old rats by means of immunocytochemistry and scanning electron microscopy. A computer-assisted image processing system showed a significant increase of the glial fibrillary acidic protein (GFAP) immunoreactivity in the internal layer (IL) and the perivascular region of the external layer (EL) of the ME with age. However, no remarkable changes in either layer could be detected employing vimentin immunoreactivity. An immunoelectron microscopic study revealed that the number of the GFAP-immunoreactive processes markedly increased with age in regions surrounding the capillaries of the EL. Therefore, it may be considered that, in both layers, the astrocytic cell bodies and associated glial processes exhibit a significant increase with age. Investigation of the microvasculature under a scanning electron microscope demonstrated the development and the sinusoidal progress of the external capillary plexus with age, but these changes were not observed in the internal capillary network of the ME. In conclusion, the results of the present study showed a close correlation between the increase of the GFAP-immunoreactive astrocytes and the development of the external capillary plexus. In addition, there was a close correlation between the increase in the GFAP-immunoreactive astrocytes and the sinusoidal progress.

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“…Immunoelectron microscopic findings for the young adult group were previously reported by Tamada et al [26].…”

Section: Immunoelectron Microscopysupporting

confidence: 66%

“…It is thought that these glial cells are involved in the regulation of the release of the releasing/inhibiting (R/I) hormones in the ME [11,13,[15][16][17]26].…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Age-related Changes in Astrocytes and Microvasculature in the Median Eminence of the Rat

Ike

Tamada

Uemura

et al. 2004

Acta Histochem. Cytochem

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Blood-to-brain communication in the hypothalamus for energy intake regulation

Morita-Takemura

Wanaka

2019

Neurochemistry International

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Morphological Interrelationship Between Astrocytes and Nerve Endings in the Rat Spinal Trigeminal Nucleus Caudalis

Zhang¹,

Tamada

Akai

et al. 2006

Okajimas Folia Anat. Jpn.

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Summary: It has been reported that the spinal trigeminal nucleus caudalis (Sp5C), which receives nociceptive information from the oro-facial regions, has four laminae. To clarify the role of glial cells in the transmission of the nociceptive information, the present study was conducted to examine the detailed distribution of astrocytes in each lamina and also to investigate a morphological interrelationship between the astrocytes and nerve endings in the rat Sp5C. After the preparation of the serial cryostat sections, immunohistochemistry for glial fibrillary acidic protein (GFAP) was employed to identify the astrocytes, and immunohistochemistry for substance P (SP), calcitonin gene-related peptide (CGRP), was used for the nerve endings. We also employed double-labeling immunofluorescence and electron microscopic immunohistochemistry for the GFAP/SP or GFAP/CGRP. GFAP-positive reactions were observed in all laminae of the Sp5C, and SP-or CGRP-positive nerve endings were observed in the lamina I and II. Additionally, we clarified the presence of GFAP/SP-or GFAP/CGRP-positive reactions by the double-labeling immunofluorescence and demonstrated the morphological interrelationship between the astrocytes and nerve endings by the double-labeling electron microscopic immunohistochemistry. These findings suggest that astrocytes might play some roles in the transmission of nociceptive information from the oro-facial region.

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Morphological Interactions between Glial Fibrillary Acidic Protein (GFAP)-like Immunoreactive Elements and Lutenizing Hormone Releasing Hormone (LHRH)-like Immunoreactive Nerve Endings in the Median Eminence of the Rat. Double Labeling Immunoelectron Microscopic Study.

Cited by 3 publications

References 28 publications

Age-related Changes in Astrocytes and Microvasculature in the Median Eminence of the Rat

Age-related Changes in Astrocytes and Microvasculature in the Median Eminence of the Rat

Blood-to-brain communication in the hypothalamus for energy intake regulation

Morphological Interrelationship Between Astrocytes and Nerve Endings in the Rat Spinal Trigeminal Nucleus Caudalis

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