Morphological heterogeneity of the rabbit collecting duct

Ridderstråle, Yvonne; Kashgarian, Michael; Koeppen, Bruce M.; Giebisch, Gerhard; Stetson, David L.; Ardito, Thomas

doi:10.1038/ki.1988.230

Cited by 92 publications

(80 citation statements)

References 22 publications

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“…Alpha-intercalated cells critically contributed to urine acidification through apically localized vacuolar H-ATPases, where the H ϩ produced from H 2 O and CO 2 via cytosolic carbonic anhydrase II is extruded into the urine (49). The remaining intracellular HCO 3 Ϫ is released into the blood by the basolateral kidney-specific isoform of the Cl Ϫ /HCO 3 Ϫ exchanger band 3/AE-1 (1,15).…”

Section: Discussionmentioning

confidence: 99%

Axial heterogeneity of vasopressin-receptor subtypes along the human and mouse collecting duct

Carmosino¹,

Brooks²,

Cai³

et al. 2007

American Journal of Physiology-Renal Physiology

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Vasopressin and vasopressin antagonists are finding expanded use in mouse models of disease and in clinical medicine. To provide further insight into the physiological role of V1a and V2 vasopressin receptors in the human and mouse kidney, intrarenal localization of the receptors mRNA was determined by in situ hybridization. V2-receptor mRNA was predominantly expressed in the medulla, whereas mRNA for V1a receptors predominated in the cortex. The segmental localization of vasopressinreceptor mRNAs was determined using simultaneous in situ hybridization and immunohistochemistry for segment-specific markers, including aquaporin-2, Dolichos biflorus agglutinin, epithelial Na channels, Tamm Horsfall glycoprotein, and thiazide-sensitive Na ϩ -Cl Ϫ cotransporter. Notably, V1a receptor expression was exclusively expressed in V-ATPase/anion exchanger-1-labeled alpha-intercalated cells of the medullary collecting duct in both mouse and human kidney. In cortical collecting ducts, V1a mRNA was more widespread and detected in both principal and intercalated cells. V2-receptor mRNA is diffusely expressed along the collecting ducts in both mouse and human kidney, with higher expression levels in the medulla. These results demonstrate heterogenous axial expression of both V1a and V2 vasopressin receptors along the human and mouse collecting duct. The restricted expression of V1a-receptor mRNA in intercalated cells suggests a role for this receptor in acid-base balance. These findings further suggest distinct regulation of renal transport function by AVP through V1a and V2 receptors in the cortex vs. the medulla. in situ hybridization; vasopressin receptors; intercalated cell ARGININE VASOPRESSIN (AVP) is the key hormone responsible for producing a concentrated urine and is secreted in high concentrations during antidiuresis. At least two different vasopressin

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Section: Discussionmentioning

confidence: 99%

Axial heterogeneity of vasopressin-receptor subtypes along the human and mouse collecting duct

Carmosino¹,

Brooks²,

Cai³

et al. 2007

American Journal of Physiology-Renal Physiology

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“…From this point the collecting ducts travel directly to the papillary tip, where the tubule fluid empties into the renal pelvis. The segments of the collecting duct are named according to the regions where they exist, but significant structural and functional differences have been identified in the different segments (13,31,40). The major divisions of the collecting duct are as follows: the cortical collecting duct (CCD); the outer medullary collecting duct (OMCD), which is subdivided into the OMCD in the outer stripe of the outer medulla (OMCD,) and the OMCD in the inner stripe of the outer medulla (OMCD,); and the inner medullary collecting duct (IMCD), which is subdivided by various authors into 2 (initial and terminal IMCD) or 3 (IMCD,, IMCD,, and IMCD,) segments (12,41).…”

Section: Figmentioning

confidence: 99%

Normal Ultrastructure of the Kidney and Lower Urinary Tract

Verlander

1998

Toxicol Pathol

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The mammalian urinary tract includes the kidneys, ureters, urinary bladder, and urethra. The renal parenchyma is composed of the glomeruli and a heterogeneous array of tubule segments that are spccialized in both function and structure and are arranged in a specific spatial distribution. The ultrastructure of the glomeruli and renal tubule epithelia have been well characterized and the relationship between the cellular structure and the function of the various components of the kidney have been the subject of intense study by many investigators. The lower, urinary tract, the ureters, urinary bladder, and urethra, which are histologically similar throughout, are composed of a mucosal layer lined by transitional epithelium, a tunica muscularis, and a tunica serosa or adventitia.The present manuscript reviews the normal ultrastructural morphology of the kidney and the lower urinary tract. The normal ultrastructure is illustrated using transmission electron microscopy of normal rat kidney and urinary bladder preserved by in ~vivo perfusion with glutaraldehyde fixative and processed in epoxy resin.

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“…Intercalated cells are interspersed between principal cells, make up 35-40% of the total cells of the CCT (1,2), and both secrete (18) and reabsorb HCO- (19). At least two subtypes of intercalated cells are present.…”

Section: Introductionmentioning

confidence: 99%

“…In the cortical collecting tubule (CCT)' regulation ofthe excretion or reabsorption ofeach of these occurs. Corresponding with the diversity of functions, the CCT is a heterogenous tissue composed of multiple cell types: principal cells and at least two types of intercalated cells (1,2).…”

Section: Introductionmentioning

confidence: 99%