Morphological evidence of collateral sprouting of intact afferent and motor axons of the rat ulnar nerve demonstrated by one type of tracer molecule

Kubek, Tomáš; Kýr, Michal; Haninec, Pavel; Šámal, Filip; Dubový, Petr

doi:10.1016/s0940-9602(04)80008-6

Cited by 25 publications

(12 citation statements)

References 19 publications

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“…Retrograde double-labelling allows detection of neurons from the donor nerve, which also projects into the end-to-side attached distal nerve segment. Both double-labelled motor and sensory neurons were found in the study, indicating that collateral sprouting occurs when an end-to-side nerve repair is made [19]. However, the extent of double-labelling was meagre.…”

Section: Discussionmentioning

confidence: 71%

End-to-side nerve repair: Attachment of a distal, compared with a proximal and distal, nerve segment

Bontioti

Kanje

Dahlin

2006

Scandinavian Journal of Plastic and Reconstructive Surgery and

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Regeneration occurs if a distal nerve segment is attached end-to-side to an intact donor nerve after the nerve has been injured. We investigated if attachment of a proximal nerve segment as well, as an extra source of axons, had any advantage over the single attachment of a distal nerve segment to the same donor nerve. In rats, a single distal radial nerve, or both the proximal and distal radial nerve segments, were attached end-to-side to the musculocutaneous nerve, and effects were examined up to eight months after the repair. Cell profiles were double-labelled, indicating recruitment of axons by collateral sprouting, but there were few such cells. There was a shift in the distribution of retrograde labelled neuronal cell profiles in spinal cord and in DRGs between the two types of repair. Both procedures resulted in axonal outgrowth and some functional recovery, but there was no improvement if a proximal nerve segment was also used as a source for axons.

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Section: Discussionmentioning

confidence: 71%

End-to-side nerve repair: Attachment of a distal, compared with a proximal and distal, nerve segment

Bontioti

Kanje

Dahlin

2006

Scandinavian Journal of Plastic and Reconstructive Surgery and

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“…Our results suggest that purely collateral sprouting at the site of coaptation [6,7,24,25] is not the only mechanism by which axons are recruited to an end-to-side nerve segment. Non-neuronal cells were activated in the part of the donor nerve trunk close to and distal to the site of end-to-side attachment in a manner similar to that after a crush injury or transection of a nerve trunk [2,16].…”

Section: Discussionmentioning

confidence: 79%

End-to-side nerve repair induces nuclear translocation of activating transcription factor 3

Bontioti

Dahlin

Kataoka

et al. 2006

Scandinavian Journal of Plastic and Reconstructive Surgery and

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We wanted to find out if any of three different types of manipulations: a piece of muscle or nerve put parallel to a nerve; an epineurial window made, or sutures inserted into a nerve, or both; or pieces of nerve sutured to an epineurial window end-to-side to the musculocutaneous or sciatic nerve, resulted in activation of activating transcription factor 3 (ATF3) in neurons and in non-neuronal cells. ATF3, a marker of cell activation, was investigated by immunocytochemistry one week after manipulation. A piece of nerve or muscle parallel to a nerve did not induce ATF3 locally in the nerve and induced ATF3 only rarely in neurons. In contrast, an epineurial window or insertion of sutures, or both, with or without attachment of a piece of nerve placed end-to-side, induced robust ATF3 expression. We conclude that an injury to a peripheral nerve trunk associated with end-to-side nerve repair, activates neurons and non-neuronal cells and may contribute to sprouting of axons into the nerve attached end-to-side.

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“…The motor neuron in the spinal cord is also thought to have a connection to the "original" muscle innervated by the donor nerve to the target muscle. This is elucidated in double-labeling studies, in which neurons in the spinal cord were found that contained a retrograde tracer from both the donor nerve and the recipient nerve (1,24,30,61).…”

Section: End-to-side Coaptation: a Critical Reviewmentioning

confidence: 99%

Results of End-to-Side Nerve Coaptation in Severe Obstetric Brachial Plexus Lesions

Pondaag

Gilbert

2008

Neurosurgery

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Morphological evidence of collateral sprouting of intact afferent and motor axons of the rat ulnar nerve demonstrated by one type of tracer molecule

Cited by 25 publications

References 19 publications

End-to-side nerve repair: Attachment of a distal, compared with a proximal and distal, nerve segment

End-to-side nerve repair: Attachment of a distal, compared with a proximal and distal, nerve segment

End-to-side nerve repair induces nuclear translocation of activating transcription factor 3

Results of End-to-Side Nerve Coaptation in Severe Obstetric Brachial Plexus Lesions

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