Morphological evaluation of HAp nanostructures and its shape-dependent protein adsorption study

Balu, Satheesh Kumar; Bargavi, P.; Chitra, S.; Kannan, Saranya; Ramadoss, Ramya; Andra, Swetha

doi:10.1016/j.matlet.2022.133491

Cited by 4 publications

(4 citation statements)

References 13 publications

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“…Two functional groups including the phosphate and hydroxyl were detected in these spectra. While the bands of 474, 561, 599, 962, 1027, and 1088 cm -1 are associated with the phosphate group the ones at 630, and 3573 cm -1 are assigned to the hydroxyl group [10,11]. Both functional groups and their band positions verify the formation of the HA structure for all the as-produced samples.…”

Section: Ft-ir Resultsmentioning

confidence: 66%

Investigation of structural properties of Pr-doped hydroxyapatites synthesized via an ultrasonic-assisted wet chemical method

Ateş

Bulut

Kayğili

et al. 2023

Journal of Physical Chemistry and Functional Materials

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In this study, the effects of the duration of ultrasonication for Pr-doped hydroxyapatite (HA) were investigated by X-ray diffraction (XRD), scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) analyses. 0.25at.%Pr-containing HA samples were prepared by the ultrasonic-assisted wet chemical route using different ultrasonication times of 0, 30, 60, 90, and 120 min. The formation of the HA phase was confirmed by the FT-IR and XRD data for each sample. The morphology was significantly affected by the duration of ultrasonication. Compared to the untreated sample, remarkable decreases in both the crystallite size and the crystallinity were observed with the duration of ultrasonication.

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Section: Ft-ir Resultsmentioning

confidence: 66%

Investigation of structural properties of Pr-doped hydroxyapatites synthesized via an ultrasonic-assisted wet chemical method

Ateş

Bulut

Kayğili

et al. 2023

Journal of Physical Chemistry and Functional Materials

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“…The XRD pattern of the Fe 3 O 4 @HAP reveals that all the diffraction peaks of Fe 3 O 4 are visible in the composite, suggesting that the Fe 3 O 4 is present in the synthesized product. In addition, some new diffraction peaks at 25.9, 31.8, 32.9, 34.1, 39.8, 43.8, 46.7, 49.5, and 53.2° corresponding to the (002), (211), (300), (202), (130), (113), (222), (213), and (004) planes of HAP are observed. These peaks are well consistent with the synthesized pure HAP.…”

Section: Resultsmentioning

confidence: 91%

Specific Adsorption of Magnetic Fe₃O₄@hydroxyapatite Nanocomposites to a Hybridized Duplex for Immobilization and Label-Free Electrochemical Biosensing of MicroRNA

Chen

Yang

et al. 2023

ACS Appl. Nano Mater.

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The unique discriminating ability of hydroxyapatite (HAP) to single- and double-stranded nucleic acid implies its potential as a biosensing material for nucleic acid analysis. Herein, a novel immobilization- and label-free electrochemical strategy has been developed for miRNA let-7a detection based on the specific adsorption of magnetic Fe3O4@hydroxyapatite (Fe3O4@HAP) to a duplex structure. Typically, the highly efficient homogeneous reaction between probe DNA (pDNA) and target miRNA let-7a produces the nucleic acid duplex, which is specifically adsorbed on the surface of Fe3O4@HAP. Thereafter, the electroactive intercalator Nile blue (NB) is introduced as the label-free signal molecule and inserts between the stacked base pairs of the pDNA–miRNA duplex. Thus, the Fe3O4@HAP bearing pDNA–miRNA and numerous intercalated NBs are adsorbed by the magnetic glassy carbon electrode, giving rise to an intense sensing signal without the need of nuclease-assisted cyclic amplification. With this advantage, the sensing system shows a wide linear range from 1.0 fM to 100 nM and a low detection limit of 0.051 fM for miRNA let-7a analysis. What is more, the synergy of the base-pairing-driven hybridization, the specific intercalation of NB to the duplex structure, and the selective adsorption of Fe3O4@HAP contribute to a high sensing specificity of the biosensing strategy. The sensing method provides a state-of-the-art strategy for the sensitive and facile bioanalysis and clinical diagnosis of miRNA.

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“…Therefore, investigating the growth principle and influencing factors of HAP crystals is of great significance for studying the biomineralization process of HAP and improving its application value [1,2]. Aqueous precipitation method is advantageous due to the low cost and simplicity [3][4][5][6][7][8][9], while the influence of adding reagents method has seldom been studied. HAP was synthesized by an aqueous precipitation method in this work.…”

Section: Introductionmentioning

confidence: 99%

Preparation and Morphology Control of Hydroxyapatite by Aqueous Precipitation

Zhai,

Lin,

Chen

et al. 2024

SJT

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Hydroxyapatite, with excellent biocompatibility, biological activity, and biological affinity, is the main inorganic component of human bones and teeth. It can promote bone tissue regeneration and has important applications in bone tissue engineering fields such as bone defect repair and bone replacement. In this study, calcium acetate solution and diammonium dihydrogen phosphate solution had been mixed to prepare calcium phosphate through aqueous precipitation reaction. The testing results of scanning electron microscopy and X-ray diffraction show that the morphology of the product can be controlled by changing the pH, the temperature, and the method of adding reactant solution dropwise. When the temperature of the reaction system is 20℃, plate-like calcium hydrogen phosphate is mainly obtained; block-like hydroxyapatite is obtained when the temperature rised to 40℃; and needle shaped hydroxyapatite is obtained at 60℃. The length and diameter of two-dimensional hydroxyapatite crystals become smaller when the temperature and the pH value of the reaction system increase.

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Morphological evaluation of HAp nanostructures and its shape-dependent protein adsorption study

Cited by 4 publications

References 13 publications

Investigation of structural properties of Pr-doped hydroxyapatites synthesized via an ultrasonic-assisted wet chemical method

Investigation of structural properties of Pr-doped hydroxyapatites synthesized via an ultrasonic-assisted wet chemical method

Specific Adsorption of Magnetic Fe₃O₄@hydroxyapatite Nanocomposites to a Hybridized Duplex for Immobilization and Label-Free Electrochemical Biosensing of MicroRNA

Preparation and Morphology Control of Hydroxyapatite by Aqueous Precipitation

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Morphological evaluation of HAp nanostructures and its shape-dependent protein adsorption study

Cited by 4 publications

References 13 publications

Investigation of structural properties of Pr-doped hydroxyapatites synthesized via an ultrasonic-assisted wet chemical method

Investigation of structural properties of Pr-doped hydroxyapatites synthesized via an ultrasonic-assisted wet chemical method

Specific Adsorption of Magnetic Fe3O4@hydroxyapatite Nanocomposites to a Hybridized Duplex for Immobilization and Label-Free Electrochemical Biosensing of MicroRNA

Preparation and Morphology Control of Hydroxyapatite by Aqueous Precipitation

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Specific Adsorption of Magnetic Fe₃O₄@hydroxyapatite Nanocomposites to a Hybridized Duplex for Immobilization and Label-Free Electrochemical Biosensing of MicroRNA