Morphological effects of autologous hsp70 on peritoneal macrophages in a murine T cell lymphoma

Abstract

Heat shock protein 70 is highly conserved cytosolic protein which have important role in growth, development, and apoptosis. Hsp70 is well-known activator of macrophages and enhances the release of specific and non-specific effector molecules that have major role in tumor destruction and immunopotentiation of host. However, morphological effects of hsp 70 has not been carried out, therefore, morphological effects of hsp 70 on murine peritoneal macrophages were examined by light microscopy and scanning electron microscopy. Thioglycolate-induced peritoneal macrophages were prepared from BALB/c mice and cultured for 24 h in the presence of the hsp70. Tumor-associated macrophages treated with 10 μg/ml were varied in shape, mostly spindle shaped, i.e., stretched bidirectionally; surface ruffles were increased and their lamellipodia was prominent which suggest that hsp 70 treatment not only enhances the functional state of the peritoneal macrophages but also initiate immense morphological changes leading to increased endothelium adherence, increased antigen uptake, and increased migration to the inflammatory site.

HOXC13 and HSP27 Expression in Skin and the Periodic Growth of Secondary Fiber Follicles from Longdong Cashmere Goats Raised in Different Production Systems

“…The research of SF in Hexi cashmere goat have also found that the activity changes of SF and the expression of Hoxc13 are closely related (He et al, ). HSP can be detected in all organisms (Kakobsson et al, ; Zhang et al, 2014; Gautam et al, ; Murphy 2013). Paul et al () studies have proved that heat stimulation can activate the expression of HSP60 and HSP70, and then activate the immune systems of goat.…”

Nanostructured titanium regulates osseointegration via influencing macrophage polarization in the osteogenic environment

“…The round shape with shorter pseudopodium in NT-30 and the stretched shape in NT-100 were consistent with previous studies that a round shape is more related to M2 predominant, while a stretched shape is more related to M1 predominant. [42][43][44] Cytoimmunofluorescence results showed that macrophages in NT-30 were prone to the M2 phenotype while cells in P and NT-100 were prone to the M1 phenotype. It was notable that the fluorescence intensity of CD163, a function-associated surface marker of M2 macrophages, is weaker than that of iNOS in general, suggesting that the intrinsic immune response elicited by topography is weaker than that influenced by chemical stimuli at the implantation site.…”

Dendritic cells pulsed with Hsp70 and HBxAg induce specific antitumor immune responses in hepatitis B virus-associated hepatocellular carcinoma

“…A previous study suggested that HSPs, including Hsp70 and Hsp90, are potent tumor-antigen sources for pulsing DCs to activate macrophages and release specific and non-specific effecter molecules, which can increase the effect of the macrophages ( 22 ). Inducible Hsp70 may function as a crosslink between the innate and adaptive immune response ( 23 , 24 ).…”