Morphological effect of electromagnetic stimulation on the skeleton of fetal or newborn mice

Hinsenkamp, Maurice; Rooze, Marcel

doi:10.3109/17453678209158548

Cited by 21 publications

(8 citation statements)

References 11 publications

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“…Our previous studies on embryonic bone, exposure to time varying electromagnetic fields (EMF) showed an acceleration of the maturation of the cartilage, which immediately precedes, in the normal physiological sequence, the enchondral ossification [14,19,28] and an acceleration of membranous ossification, which are known effects of BMP-2 [17,19,29].…”

Section: Discussionmentioning

confidence: 99%

“…& In limb buds of mice embryos exposed in vitro, an increased concentration of acid glycosaminoglycans in the cartilaginous matrix of bones [14,19,28] (Fig 1); & In chicken embryos exposed in vivo, a relative acceleration in the ossification at the primary ossification point [17,19,29]; & In quail embryos, a relation between the ossification rate and the amplitude of local electric fields [18,19].…”

Section: Introductionmentioning

confidence: 99%

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Bone Morphogenic Protein--mRNA upregulation after exposure to low frequency electric field

Hinsenkamp

Collard

2011

International Orthopaedics (SICOT)

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Purpose For many years, our laboratory has been investigating different biological substrates for the effects of electromagnetic stimulation proposed in orthopaedic treatments. The results show an acceleration of differentiation at the expense of proliferation. This study using microarray analysis is focused on the cellular mechanisms involved. Methods A microarray analysis (Affymetrix) allowing the screening of the expression of 38,500 genes was used on epidermal cells sampled from three different human donors and distributed within each donor in seven groups of 12 explants, stimulated at different times, to compare control. Modifications of the expression of BMP-2, 4 and 7 were studied at days four, seven and 12.Results The expression of BMP-2 was significantly increased at day 12 on the stimulated samples. J 4 and J 7 did not show any significant difference nor did the expression of BMP-4 and 7 at the different times. Conclusion The results obtained in previous experiments on cellular substrates, bone embryonic tissue and clinical series were all consistent with the increase of BMP-2. Other publications have confirmed an increase of BMP-2 under electric or electromagnetic stimulation. The increase of BMP-2 appears as an effect of the electromagnetic field stimulations applied in orthopaedics. This observation contributes towards possible indications and a better understanding of the cellular mechanism.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bone Morphogenic Protein--mRNA upregulation after exposure to low frequency electric field

Hinsenkamp

Collard

2011

International Orthopaedics (SICOT)

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“…It was previously shown (Rooze, 1979 ;Hinsenkamp, 1982) that electromagnetic stimulation of developing mouse limb buds grown zn vrtro induces macro-and microsccpic changes in skeletal tissues. Even though they were not statistically conclusive, the results obtainzd showed that the treatment obviously influences the rn vttvo behaviour of differentiating tissues.…”

Section: Discussionmentioning

confidence: 99%

“…Previous experiments on the effects of electromagnetic stimulation on in vitvo cultivated limbs have revealed some morphological modification such a thicker proliferative layer of chondrocytes, a better alignment of the trabeculae and a better configuration of the joint cartilage (Rooze M., Hinsenkamp, 1979Hinsenkamp, , 1982.…”

Section: Introductionmentioning

confidence: 99%

Histochemical modifications induced in vitro by electromagnetic stimulation of growing bone tissues

Rooze¹,

Hinsenkamp²

1982

Acta Orthopaedica Scandinavica

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“…The joint was opened under sterile conditions within 3 h after slaughter, and pieces of cartilage were collected by dissection. Minced cartilage pieces were subjected to enzymatic digestion in serum-free Dulbecco's modified Eagle's medium, (DMEM, GIBCO, Grand Island, NY, U.S.A.) containing clostridial collagenase type I1 and type IV (Worthington, Freehold, NJ, U.S.A.) (1 mg/ml each) (25). Digestion was carried out overnight at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Time‐varying magnetic fields: Effects of orientation on chondrocyte proliferation

Elliott

Smith

Block

1988

Journal Orthopaedic Research

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The purpose of this study was to determine the effect of orientation of pulsed electromagnetic fields (PEMFs) on cellular proliferation and extracellular matrix synthesis. Bovine articular chondrocytes were cultured in PEMFs (repetitive pulse at 72 Hz) generated using Helmholtz coils oriented either parallel (horizontal) or perpendicular (vertical) to the plane of cell adhesion. Dissipation of signal energy in the form of heat increased the temperature of the PEMF coils by 2 degrees C and the tissue culture medium by 1 degree C. Therefore, control coils, which emitted no PEMFs, were heated to the temperature of PEMF coils by circulating water. Chondrocytes were cultured in 16-mm-well culture plates, and the data for individual wells were pooled as triplicates. Although not observed by microscopic examination of individual wells, positionally dependent electric field effects may be minimized by this approach. PEMFs generated by coils oriented vertically significantly decreased chondrocyte proliferation. The effect was dependent on the concentration of serum in the culture media. At 3% serum concentration, the total cell number attained after 10 days of culture was reduced by 50% in stimulated cultures when compared with controls. At 5% serum concentration, there was no effect. PEMFs applied by coils oriented horizontally did not alter proliferation of articular chondrocytes. PEMFs had no effect on synthesis of extracellular matrix by chondrocytes plated at high density, irrespective of orientation.

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Morphological effect of electromagnetic stimulation on the skeleton of fetal or newborn mice

Cited by 21 publications

References 11 publications

Bone Morphogenic Protein--mRNA upregulation after exposure to low frequency electric field

Bone Morphogenic Protein--mRNA upregulation after exposure to low frequency electric field

Histochemical modifications induced in vitro by electromagnetic stimulation of growing bone tissues

Time‐varying magnetic fields: Effects of orientation on chondrocyte proliferation

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