Morphological diversity of cultured cold-active lytic bacteriophages isolated from the Napahai plateau wetland in China

Ji, Xiuling; Zhang, Chunjing; Kuang, Anxiu; Li, Jiankai; Cui, Yinshan; Qin, Kunhao; Lü, Lin; Cheng, Benxu; Zhang, Qi; Wei, Yunlin

doi:10.1007/s12250-015-3674-4

Cited by 6 publications

(1 citation statement)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, wetlands are usually considered the “kidney” of the earth. Since these biogeochemical processes are mostly mediated by microbes 19 , wetlands are regarded as the hotspots for studying microbial ecology 18 ; however, assessments of virus or phage diversity in wetlands are limited 20,21 . In this study, by targeting the biomarker gene phoH , we surveyed phage diversity and distribution in several wetland sediments in northeast China using culture-independent PCR, cloning and Sanger sequencing methods.…”

Section: Introductionmentioning

confidence: 99%

Survey of the bacteriophage phoH gene in wetland sediments in northeast China

Sun

Liu

et al. 2019

Sci Rep

View full text Add to dashboard Cite

PhoH is a host-derived auxiliary metabolic gene that can be used as a new biomarker for surveying phage diversity in marine and paddy waters. However, the applicability of this gene in other environments has not been addressed. In this paper, we surveyed the phoH gene in four wetland sediments in northeast China. DNA was extracted directly from sediments and used for PCR amplification with the degenerate primers vPhoHf and vPhoHr. In total, 44 and 58 phoH sequences were identified as belonging to bacteria and phages, respectively, suggesting that this primer set is not highly specific to the phage phoH gene. A BLASTp search showed that the 58 phage phoH sequences had the highest identity to the known viral sequences, ranging from 48% to 100%. Phylogenetic analysis showed that all phage sequences from wetlands distributed into the previously designated Groups 2, 3, 4 and 6. In addition, two new subgroups, Groups 2c and 4c, which contained sequences exclusively from wetlands, were detected in this study. Nonmetric multidimensional scaling analysis showed that the phage phoH assemblage from a coastal wetland was similar to that in marine environments, while the phage phoH assemblage from a lake wetland was similar to that in paddy waters. These findings indicated that different types of wetlands had distinct phage phoH compositions.

show abstract