Morphological description and DNA barcoding of Hydrobaenus golovinensis sp. nov. (Diptera: Chironomidae: Orthocladiinae) from the Russian Far East

Abstract

Illustrated descriptions of adult male, pupa and fourth instar larva, as well as DNA barcoding results of Hydrobaenus golovinensis sp. nov. in comparison with closely related species H. majus Makarchenko et Makarchenko and H. sikhotealinensis Makarchenko et Makarchenko from the Russian Far East are provided. Partial mitochondrial COI gene (DNA barcoding) of the new species has been sequenced and uploaded to GenBank. Comparisons with corresponding regions of COI between H. golovinensis and other species in the genus produce K2P genetic distances of 10.3–14.3%, the values well above those associated with intraspecific variation. In contrast, genetic distances among 18 specimens are all within the range of 0–3.5%. The ML tree is also constructed using DNA barcodes obtained in the present study and those of other species of Hydrobaenus Fries from GenBank.

An updated checklist of Chironomidae (Diptera) from the Amur River basin (Russian Far East)

“…Abiskomyia korbokhon, Propsilocerus amurensis, Paralimnophyes dolgikh, Hydrobaenus golovinensis, Tokunagaia logutini, Chaetocladius antipovae, С. egorych, C. fedotkin, С. yavorskayae, Parasmittia bidzhanica, Tsudayusurika safonikha and Tvetenia bidzhanica. Abiskomyia korbokhon occurs only in oligotrophic Korbokhon Lake, which is situated at an altitude of 1160 m above sea level in the upper reach of the Bureya River (Middle Amur) (Makarchenko and Makarchenko, 2015b); P. amurensis is also known only from its type locality, the Kadi River near the Bolon Nature Reserve (Lower Amur) (Makarchenko and Makarchenko, 2009b); P. dolgikh, H. golovinensis and T. logutini are reported from running waters of the Bolshekhekhtsirsky Nature Reserve (Lower Amur) (Makarchenko et al, 2015(Makarchenko et al, , 2017bYavorskaya et al, 2017). The other above-listed species occur only at the springs of the Bidzhan River basin (Middle Amur) (Makarchenko et al, , 2017a.…”

“…Adults of some species were reared from larvae in the field. In addition, DNA barcoding (COI (Makarchenko and Semenchenko, 2014;Makarchenko et al, 2015Makarchenko et al, , 2017aMakarchenko et al, , 2017b. The techniques of preservation of material, slide-mounting and identification were used as described by Makarchenko (2006).…”

“…Further study on the food chains in fish populations conducted by the Amur branch of TINRO from 1948TINRO from to 1982 Makarchenko et al (2005), was then upgraded to 404 species by Makarchenko and Makarchenko (2008a) and Makarchenko et al (2008). During the last decade, the new data were obtained on the fauna, taxonomy and distribution of chironomids in the Amur River basin and were described a news species by us, based on both traditional morphological and molecular genetic methods (Makarchenko and Makarchenko, 2007, 2008a, 2008b, 2008c, 2009a, 2009b, 2010a, 2010b, 2010d, 2012a, 2012b, 2012c, 2015a, 2015bMakarchenko and Semenchenko, 2014;Makarchenko et al, 2005Makarchenko et al, , 2015Makarchenko et al, , 2017aMakarchenko et al, , 2017b.…”

Taxonomic notes on the genus Hydrobaenus with description of H. simferopolus sp. nov. from Crimea (Diptera: Chironomidae)

“…Knowledge on the taxonomy, geographical distribution and ecology of the known Hydrobaenus species from Europe and the Palaearctic Region was extensively highlighted by: FRIES (1830FRIES ( , 1831, SAETHER (1976), MOUBAYED (1985), CRANSTON et al (1989), LANGTON & COBO (1992), SASA & KIKUCHI (1995), ASARI et al (2004), CRANSTON et al (2007), MAKARCHENKO & MAKARCHENKO (2009, 2010, 2011, 2017, ZERGUINE & ROSSARO (2010), BARANOV (2011a,b), ASHE & O'CONNOR (2012), ANDERSEN et al (2013); SAETHER & SPIES (2013), MAKARCHENKO et al (2017). In this paper, four Hydrobaenus species are examined and diagnosed based on material composed of male adults collected in Ukraine, Lebanon, and France.…”

Morphological description and DNA barcoding of Chaetocladius (Chaetocladius) elisabethae sp. nov. (Diptera: Chironomidae: Orthocladiinae) from the Moscow Region

“…nov. from the Moscow Region are provided in comparison with closely related species of Chaetocladius s. str. The DNA barcode corresponding to the 650-bp fragment of the mitochondrial gene cytochrome c oxidase I (COI) has been identified as the core of a global bioidentification system at the species level (Hebert et al 2003) and has proved to be useful in the subfamily Orthocladiinae (Silva & Wiedenbrug 2014, Makarchenko et al 2015, 2017a, 2017b.…”