Morphological, cytochemical and ultrastructural aspects of blood cells in freshwater stingray species in the middle Rio Negro basin of Amazonian Brazil

Abstract

In the present work, we examined the morphology, dimensions, cytochemical staining reactions and ultrastructure of blood cells from three freshwater stingray species, Potamotrygon wallacei, Potamotrygon motoro and Paratrygon aiereba, living in the waters of the middle Rio Negro basin (Barcelos, Amazonas, Brazil). We identified erythrocytes, erythroblasts, thrombocytes and four types of leukocytes (basophils, heterophils, lymphocytes and monocytes) in the blood of these stingray species. In all the freshwater stingray species studied, the shapes and dimensions of these cells were similar to those of marine elasmobranchs. Positive PAS staining occurred in heterophils and thrombocytes, and weak staining occurred in lymphocytes and monocytes, while metachromasia only occurred in basophils. Positive Sudan Black B staining was observed in thrombocytes and lymphocytes, and weak staining occurred in heterophils. Basophils and heterophils were the only cells with positive bromophenol blue staining, while no peroxidase staining was observed in any of the four leukocyte types. This is the first study to establish the dimensions and cytochemical staining profiles of blood cells in Amazonian stingray species. Because these elasmobranch species are exported as ornamental fish to countries worldwide, this study can contribute to establishing standards for blood constituents that may be helpful in assessing the health and welfare of these fish in artificial systems.

Observations on the cytomorphology and ultrastructure of the peripheral blood cells of native cattle (Zobawng) of Mizoram, India

“…The morphological characteristics of the various blood cells have been reported earlier in buffalo calves, goats, Asiatic black bear, pig, and donkey (Mehta, 2010; Menaka & Singh, 2006; Mrigesh, 2011; Salakij, Salakij, Narkkong, Trongwonsa, & Pattanarangsan, 2005; Singh, 2000). The ultrastructural studies of blood cells from several household animals have been described, such as by Sonoda and Kobayashi (1970) in canines, Singh et al (1997) in camel, Singh (2000) in buffalo calves, Salakij, Salakij, Narkkong, Trongwonsa, and Pattanarangsan (2005) in black bear, Prihirunkit et al (2007) in fishing cat, Sakulwira et al (2008) in macaque, Mehta (2010) in pig, Mrigesh (2011) in donkey, Mehta and Guha (2015) in cattle, Al‐Jashamy and Sawad (2016) in camel, Mehta and Sinha (2019) in horse, dog, and de Oliveira et al (2021) in freshwater stingray species. There is no available literature on the cytomorphological and ultrastructural characterization of the blood of native cattle (Zobawng) of Mizoram, India.…”

Application of haematology parameters for health management in fish farms

“…No monocytes were found in Dolly Varden ( Salvelinus malma ), white‐spotted Charr ( Salvelinus leucomaenis ), rainbow trout or golden trout. The number of eosinophils in fish is approximately 0%–5%, but no eosinophils were found in grass carp, blunt snout bream and other fish 241,250,251 . The number of basophils in fish is even less and these cells are not found in most fish.…”

“…The number of eosinophils in fish is approximately 0%-5%, but no eosinophils were found in grass carp, blunt snout bream and other fish. 241,250,251 The number of basophils in fish is even less and these cells are not found in most fish. For example, the number of basophils in Nile tilapia was 2.67 ± 0.33%.…”

Hematological parameters and plasma biochemistry of tambaqui Colossoma macropomum from fish farms in the Amazon region

“…Slides (n: 3/collected fish) with blood extensions were prepared and stained with May-Grunwald, Giemsa, Wright and methanol (MGGW) [15]. The material was used to count total leukocytes and thrombocytes [16] and for the differential count of leukocytes based on 200 leukocyte types.…”