Morphological Control of Room-Temperature Ionic Liquid Templated Mesoporous Silica Nanoparticles for Controlled Release of Antibacterial Agents

Trewyn, Brian G.; Whitman, Chad M.; Lin, Victor S.-Y.

doi:10.1021/nl048774r

Cited by 416 publications

(293 citation statements)

References 18 publications

(16 reference statements)

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“…[1][2][3][4] Furthermore, recent reports on the design of functional mesoporous silica materials by decorating the pore surface with organic or inorganic moieties that could serve as gating devices to regulate the release of guest molecules under the control of several different external stimuli, such as chemicals, [5][6][7][8][9] temperature, 10 redox reactions, 11,12 and photoirradiation, 13,14 have highlighted the potential of utilizing this kind of nanodevice for many controlled release applications.…”

Section: Introductionmentioning

confidence: 99%

“…We and others have demonstrated that, by controlling the particle size, shape, and surface functionality of the mesoporous silicas, these structurally defined materials can be efficiently endocytosed by live cells with high biocompatibility in vitro and serve as delivery vehicles for the controlled release of genes and membrane-impermeable chemicals. 3,[5][6][7] Also, several enzymes have been shown to be functional without being denatured inside the frameworks of mesoporous silicas, such as MCM-41 and SBA-15 silicas. [15][16][17][18][19][20][21][22][23] However, to the best of our knowledge, there were only two prior reports on the controlled release of proteins from SBA-15-type mesoporous silica materials under nonphysiological conditions.…”

Section: Introductionmentioning

confidence: 99%

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Mesoporous Silica Nanoparticles for Intracellular Delivery of Membrane-Impermeable Proteins

2007

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An MCM-41-type mesoporous silica nanoparticle (MSN) material with a large average pore diameter (5.4 nm) is synthesized and characterized. The in vitro uptake and release profiles of cytochrome c by the MSN were investigated. The enzymatic activity of the released protein was quantitatively analyzed and compared with that of the native cytochrome c in physiological buffer solutions. We found that the enzymes released from the MSNs are still functional and highly active in catalyzing the oxidation of 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) (ABTS) by hydrogen peroxide. In contrast to the fact that cytochrome c is a cell-membrane-impermeable protein, we discovered that the cytochrome c-encapsulated MSNs could be internalized by live human cervical cancer cells (HeLa) and the protein could be released into the cytoplasm. We envision that these MSNs with large pores could serve as a transmembrane delivery vehicle for controlled release of membrane-impermeable proteins in live cells, which may lead to many important biotechnological applications including therapeutics and metabolic manipulation of cells. Abstract: An MCM-41-type mesoporous silica nanoparticle (MSN) material with a large average pore diameter (5.4 nm) is synthesized and characterized. The in vitro uptake and release profiles of cytochrome c by the MSN were investigated. The enzymatic activity of the released protein was quantitatively analyzed and compared with that of the native cytochrome c in physiological buffer solutions. We found that the enzymes released from the MSNs are still functional and highly active in catalyzing the oxidation of 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonate) (ABTS) by hydrogen peroxide. In contrast to the fact that cytochrome c is a cell-membrane-impermeable protein, we discovered that the cytochrome c-encapsulated MSNs could be internalized by live human cervical cancer cells (HeLa) and the protein could be released into the cytoplasm. We envision that these MSNs with large pores could serve as a transmembrane delivery vehicle for controlled release of membrane-impermeable proteins in live cells, which may lead to many important biotechnological applications including therapeutics and metabolic manipulation of cells.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mesoporous Silica Nanoparticles for Intracellular Delivery of Membrane-Impermeable Proteins

2007

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“…16 They have been successfully used to deliver drugs, 17,18 proteins 19 and nucleic acids. 20,21 Most recent efforts have been focused on their biological targeting in order to achieve site-specific delivery.…”

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confidence: 99%

Targeted Cytosolic Delivery of Cell-Impermeable Compounds by Nanoparticle-Mediated, Light-Triggered Endosome Disruption

Febvay

Marini

Belcher³

et al. 2010

Nano Lett.

109

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Nanoparticle (NP)-mediated drug delivery typically relies on cargo release to occur passively or in response to environmental stimuli. Here we present a delivery method based on light-activated disruption of intracellular vesicles after internalization of biofunctionalized mesoporous silica nanoparticles loaded with cargo. This method combines the power of targeted delivery with the spatiotemporal control of light activation. As an example, we delivered a cell-impermeable fluorescent compound exclusively to the cytosol of multidrug resistant cancer cells in a mixed population. KeywordsMesoporous silica; nanoparticles; P-glycoprotein; drug delivery; photodynamic therapy; endosomeThe potential of nanomaterials for drug delivery has been extensively explored in recent years. [1][2][3][4] Such efforts have been driven to a large extent by the need to reduce side effects in anticancer drugs (such as doxorubicin's severe cardiotoxicity) via tissue-specific targeting. 5 Beyond enhanced targetability, NP-encapsulation of drugs may also provide protection against premature degradation and enable efficient delivery of substances with poor inherent solubility or membrane permeability. Important determinants of the success of NP-mediated drug delivery are biocompatibility, circulation time, immunogenicity, specific targeting, © 2010 American Chemical Society * To whom correspondence should be addressed. dclapham@enders.tch.harvard.edu. HHMI Author Manuscript HHMI Author Manuscript HHMI Author Manuscripttiming of cargo release and, ideally, access to intracellular compartments. Careful tuning of NP size, especially in the 10-100 nm range, enables control of circulation time, 6,7 as well as passive tumor accumulation due to the "enhanced permeability and retention" effect. 8 Particle size also affects NP cellular uptake. 9 A highly desirable feature of NP-based delivery platforms is precise temporal control of compound release. This can be regulated by incorporating release mechanisms triggered by environmental stimuli such as pH, 10 temperature, 11 or enzymatic reactions. 12 Another important way to control payload delivery for membrane-impermeable drugs is to regulate access to the cytosol, which usually requires escape from the endolysosomal compartment where particles are clustered following receptor-mediated endocytosis. Here we present a method for precise spatial and temporal control over cytosolic delivery of compounds that would otherwise be cell-impermeable by incorporating a light-triggered endosomal escape mechanism within a cell targetable mesoporous silica carrier.Among potential nanocarriers, mesoporous silicate materials 13-15 have demonstrated great potential for biomedical applications due to their biocompatibility, ease of functionalization, and large surface-to-volume ratio. 16 They have been successfully used to deliver drugs, 17,18 proteins 19 and nucleic acids. 20,21 Most recent efforts have been focused on their biological targeting in order to achieve site-specific delivery. 16,22 Size contro...

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“…Zhou et al and Zhang et al utilize 1-butyl-3-methylimidazolium tetrafluoroborate (C 4 MimBF 4 ) to synthesize mesoporous silica via a new mechanism, known as hydrogen bond-co-π-π-stack [1,32]. Despite these many researches conducted on usage of ILs as template, only one research was able to use ILs to develop MSNs and in this research, it was found that MSNs morphology are dependent on the type of ILs used [33].…”

Section: Introductionmentioning

confidence: 80%

Monodispersed mesoporous silica nanospheres based on pyridinium ionic liquids

2018

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In the past decades, ionic liquids (ILs) have garnered a lot of attention especially in the field of green chemistry due to their unique properties which help to solve the problems posed by organic solvents. Interestingly, their applications are not limited to that only as ILs have the potential to become alternative templates in the development of mesoporous silica nanoparticles (MSNs). This work reported the usage of a series of pyridinium ILs as template in the synthesis of monodispersed mesoporous silica nanosphere (MNSs) via two methods. Both syntheses utilize triethanolamine (TEA) as the base catalyst where in one method the TEA undergoes pre-treatment process while the other did not. Besides that, the effects of pyridinium ILs alkyl chain length were also investigated. MNSs generated via both methods exhibit spherical morphology and decreasing average particles size with increasing alkyl chain length of pyridinium ILs. The MNSs porosity were further analyzed through nitrogen sorption analysis where the surface area were in between 71.85 and 525.02 m 2 g −1 and the pore volume was up to 1 cm 3 g −1 .

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Morphological Control of Room-Temperature Ionic Liquid Templated Mesoporous Silica Nanoparticles for Controlled Release of Antibacterial Agents

Cited by 416 publications

References 18 publications

Mesoporous Silica Nanoparticles for Intracellular Delivery of Membrane-Impermeable Proteins

Mesoporous Silica Nanoparticles for Intracellular Delivery of Membrane-Impermeable Proteins

Targeted Cytosolic Delivery of Cell-Impermeable Compounds by Nanoparticle-Mediated, Light-Triggered Endosome Disruption

Monodispersed mesoporous silica nanospheres based on pyridinium ionic liquids

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