Morphological confocal laser scanning microscope evaluation of four different “etch and rinse^|^quot; adhesives in post endodontic restoration

Marigo, Luca; Lajolo, Carlo; Castagnola, Raffaella; Angerame, Daniele; Somma, Francesco

doi:10.4012/dmj.2012-022

Cited by 8 publications

(10 citation statements)

References 18 publications

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“…Analysis of the micromechanical interaction of the adhesive interface was performed using confocal microscopy. This method evaluates the micropermeability and the sealing ability of the resin tags at the adhesive interface by using fluorescent dyes in the dental substrate and/ or resinous material [ 36 ], allowing for the evaluation of the subsurface. This is a non-destructive method which offers more details regarding the identification of the structures that constitute the adhesive interface [ 29 ] with less artefacts and decreased halo formation in the image when compared to scanning electronic microscopy [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Analysis of the bond interface between self-adhesive resin cement to eroded dentin in vitro

et al. 2018

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The purpose of this study was to evaluate the bonding interface between a self-adhesive resin cement to in vitro eroded dentin. Seventy-two third molars were used and divided into two groups: sound dentin and in vitro eroded dentin. The in vitro erosion was performed following a demineralization protocol, in which the specimens were immersed in a demineralizing solution for 2 minutes per cycle and remineralizing solution for 10 minutes per cycle for 9 days. Both groups were submitted to four dentin surface treatments: control group (without any treatment), 2% chlorhexidine, 20% polyacrylic acid, and 0.1 M EDTA (n = 9). Blocks of resin-based composite were bonded with RelyX U200 self-adhesive resin cement applied on the pretreated dentin surfaces. The teeth were sectioned into beams (1mm2) and submitted to microtensile bond strength testing to evaluate the bond strength of self-adhesive resin cement to dentin after 24 hours and 8 months of immersion in artificial saliva. Three specimens of each group were longitudinally cut and evaluated using confocal laser scanning microscopy to analyze the dentin/cement interface. Eroded dentin showed higher bond strength values when compared to sound dentin for the 2% chlorhexidine group (p = 0.03), 24 hours after adhesion. When considering eroded dentin, the 0.1M EDTA group showed higher bond strength values with a statistically significant difference only for the control group (p = 0.002). After 8 months of storage, the present results showed that there was no statistically significant difference between the two substrates for all experimental groups (p>0.05). Analysis of the microscopy confocal showed different types of treatments performed on dentin generally increased tags formation when compared to the control group. The eroded dentin showed a significant increase in density and depth of resinous tags when compared to sound dentin. The storage of samples for 8 months seems to have not caused significant degradation of the adhesive interface.

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Section: Discussionmentioning

confidence: 99%

Analysis of the bond interface between self-adhesive resin cement to eroded dentin in vitro

et al. 2018

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“…Thanks to the use of Rhodamine B, this technique allows great precision in assessing the depth and number of tubules showing penetration, as well as the perimeter of the canal in which the marked material is located. Bitter et al in 2009 (18) and Marigo et al in 2012 (20) demonstrated that fluorochrome binding with the cements or adhesive-systems to which they are added does not modify the properties of the latter (20,21). With this examination process it is not necessary to subject the samples to any drying processes (as occurs with scanning electron microscopy, for example) that might result in material disadaptation or scaling.…”

Section: Discussionmentioning

confidence: 99%

Root canal adaptation and intra-tubular penetration of three fiber-post cementation systems

et al. 2018

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BackgroundTo measure the penetration of three bonding systems for the luting of fiber glass posts in endodontically treated teeth, using confocal laser scanning microscopy (CLSM).Material and MethodsA total of 30 maxillary incisors were shaped with the Mtwo system and filled with gutta-percha and Top Seal cement. The sample was divided into three groups (ni=10) according to the bonding system used to cement the posts: Group 1 (Prime&Bond NT and Rebilda DC with a total-etch technique); Group 2 (Futurabond DC and Rebilda DC with a dentin self-etch technique); and Group 3 (BisCem self-adhesive cement). Rhodamine B was added to the bonding systems to allow visualization by CLSM. Three 1-mm thick cross-sections were obtained of each root at a distance of 2, 5 and 8 mm from the coronal limit of the root. The specific software of the CLSM system was used to measure the percentage of the root canal perimeter showing penetration of the bonding system in the dentinal tubules, together with the maximum depth of penetration. Comparison between groups were made by Kruskal Wallis test, and comparison two by two groups with Mann-Whitney U-test.ResultsDepth of penetration of the resin tags, were distributed from greater to lesser depth as follows: BisCem > Prime&Bond NT > Futurabond DC. BisCem showed significantly greater penetration in the middle and apical thirds than the rest of the systems (906.14±67.42 and 699.27±76.26 µm, respectively). The percentage perimeter exhibiting penetration in the coronal third was significantly greater with BisCem versus Futurabond DC (56.08±7.24 and 44.38±5.23%, respectively). No significant differences were recorded in the middle and apical thirds among the three systems.ConclusionsBisCem resulted in greater depth of intratubular penetration at all studied levels. The percentage perimeter of the canal showing penetration was similar for all the bonding systems. Key words:Fiber post luting, sealer adaptation, confocal laser scanning microscope.

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“…Regarding the interface with dentin, the Prime & Bond 2.1/Self Cure + Enforce presented a uniform formation of tags with gaps ( Figure 6A). The confocal microscopy analysis also observed that the use of etch & rinse adhesive systems produced good results in terms of density and the quality of resin tags [13,15]. Polymerization shrinkage stresses that were generated because of the highly unfavorable cavity configuration factor of the post spaces may affect the interfacial strength of resin-based material, causing gaps in the adhesive interface ( Figure 6A) [4,16].…”

Section: Groups With Asterisks Statistically Differ From the Others mentioning

confidence: 97%

“…restored with adhesive materials, including the bonding process of glass fiber posts and resin cements to dentin [12,13]. However, different methodologies have been used according to the objectives of the study.…”

Section: Groups With Asterisks Statistically Differ From the Others mentioning

confidence: 99%

Confocal laser scanning microscopy investigation of bond interfaces involved in fiber glass post cementation

Fagundes

Somensi²,

Santos

et al. 2014

BDS

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Objective: This confocal microscopy study evaluated the cement/dentin and cement/post interfaces along the root canal walls when fiber glass posts were bonded to dentin using different types of cements.Material and methods: Thirty endodontically treated premolars were divided into 3 groups according to the adhesive materials used in the bonding procedure: Prime & Bond 2.1/Self Cure + Enforce, RelyX Unicem and RelyX Luting. Rhodamine B dye was incorporated in the luting materials for the cementation of the fiber glass posts (Exacto, Angelus) to dentin. Three transversal slices (apical, middle and coronal) were examined under confocal laser scanning microscopy. Statistical analysis was performed using the Kappa, Kruskal-Wallis and Dunnet tests.Results: The Prime & Bond 2.1/Self Cure + Enforce presented a uniform formation of tags in the dentin but gaps in the cement/dentin interface. The RelyX Unicem and RelyX Luting presented an adhesive interface with a fewer amount of gaps, but showed shorter tag formation than the Enforce system. All cements presented the same pattern of bubbles inside the cements. The RelyX Luting presented a greater amount of cracks inside the cement in comparison with the other cements in the coronal third, while no difference was observed between RelyX Unicem and Enforce. The RelyX Luting showed the lowest quantity of cement penetration into the post.Conclusions: In general, the quality of bonding interfaces of fiber posts luted to root canals was affected by both location and type of cement.

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Morphological confocal laser scanning microscope evaluation of four different “etch and rinse^|^quot; adhesives in post endodontic restoration

Cited by 8 publications

References 18 publications

Analysis of the bond interface between self-adhesive resin cement to eroded dentin in vitro

Analysis of the bond interface between self-adhesive resin cement to eroded dentin in vitro

Root canal adaptation and intra-tubular penetration of three fiber-post cementation systems

Confocal laser scanning microscopy investigation of bond interfaces involved in fiber glass post cementation

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