Morphological, Chemical Characters and Genetic Analysis Discrimination of Five Naturalized Populus Species Inhabiting Four Governorate Territories of Egypt

Abstract

Populus is a fast growing tree that attract the attention of people. This genus provides environment protection due to their ability in carbon sequestration, phytoremediation and wildlife inhabitant. Populus species are widely used as a source of wood, veneer, paper and bioenergy. This survey study aimed to identify the current status of Populus species inhabiting four different governorates of the Egyptian territories. In this survey, four different locations were surveyed, i.e. Cairo, Giza, Qaliobia and Gharbia governorates. According to the site survey, five species of Populus were found. Research findings indicate that the length and diameter of the trees varied according to various species in various localities. Selected trees of various species were subjected to different morphological, chemical parameters and genetic assessments. P. deltoides from Giza in addition to P. deltoides and P. nigra from Cairo gave somewhat close relation in their vegetative parameters. Furthermore, both P. nigra and P. alba from Gharbia location gave high similarity due to overall vegetative parameters and also both P. euramericana female from the Gharbia and P. nigra from Qaliobia. Genetic diversity was analyzed using molecular markers. Three different Inter-simple sequence repeat (ISSR) primers were used for the reaction (17899A, 17899B and HB13). ISSR primers markers yielded 67.5% polymorphic loci among the surveyed species and cluster analysis enabled separation of these populations on the basis of their genetic distances. P. euramericana female and male from Giza showed close relation at morphological level. From this study it can be concluded that: firstly; leaf parameters i.e. leaf area width and length were suitable for identifying the differences among species, and secondly; genetic analysis using molecular markers was enough to discriminate between species. Our results finally suggest that Populus species possess genetic variation and can adapt to new environmental conditions, which in its self-valuable information would potentially lead to promising applications.

Metal Based-oxide Nanoparticles Assisted the Uptake of the Essential Elements and Phytoremediation of Populus Alba : Essential Metabolic Processes and Genetic Stability

“…The acclimatization of P. alba culture was performed by Ahmed et al (Hewidy et al 2019) andEl-Sayed et al (El-Sayed et al 2019) to obtain the best growth conditions. Gomes and co-workers explored the morphological, anatomical parameters and chlorophyll content for affected vegetation area surrounding the mining company produced heavy metals such as Zn, Cd and Pb (Gomes et al 2011).…”

“…Explants of Populus alba nodal segments were cultured and acclimatized on Murashige and Skoog medium (MS medium) as reported by Ahmed and co-workers (Hewidy et al 2019). Each nano-metal oxides of the heavy element (i.e., Fe, Zn, Mn) that used as micronutrients were prepared in three different concentrations of (i.e., 20, 40, 60 mg/L).…”

Metal-based-oxide nanoparticles assisted the in vitro culture growth of Populus alba as micronutrients: essential metabolic processes and genetic stability

“…Explants of Populus alba Lauche (Populus alba L.) nodal segments were cultured and acclimatized on Murashige and Skoog medium (MS medium) as previously reported. 51 Each nano-metal oxide (i.e., Fe, Zn, Mn) used as a micronutrient was prepared at three different concentrations (i.e., 20, 40, and 60 mg L −1 ). These different concentrations were inoculated in MS jars separately.…”

Vital Parameters Assessments of Starvation Tolerance of in vitro Populus alba Culture

“…In various studies, some plants' genetic diversity has been investigated using different molecular markers [13]. The most significant uses have been the study of molecular variability and phylogenetic relationships, the marker-assisted selection, varietal identification, quantitative trait loci (QTLs), or the map-based cloning of genes [14].…”