Morphological-, chemical- and RAPD-PCR evaluation of eight different Ocimum basilicum L. gene bank accessions

Bernhardt, Botond; Fazekas, G.A.; Ladányi, Márta; Inotai, Katalin; Zámbori-Németh, É.; Bernáth, J.; Szabó, Krisztina

doi:10.1016/j.jarmap.2014.03.002

Cited by 8 publications

(8 citation statements)

References 16 publications

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“…The polymorphic banding pattern in the callus of O. basilicumpossibly indicates that there is a negligible level of genetic variation. Bernhardt et al (2014) have evaluated the RAPD banding pattern of eight different O. basilicum gene bank accessions and have reported similar result. Anamika et al (2010) reported the same condition in Pogostemoncablin.…”

Section: C B Amentioning

confidence: 62%

Effect of Indole -3- Acetic Acid on Callus Induction From Leaf Explant of Ocimum Basilicum Linn.

V.N¹,

Sirangeevi²,

Deeksha³

et al. 2015

krj

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Ocimum basilicum L. belongs to the family Lamiaceae; contains plenty of phytochemicals with significant nutritional as well as health benefits. Antioxidant activity of methanol extract of Ocimum basilicum callus was evaluated using various antioxidant assays. Proliferated callus was obtained on half-strength MS media supplemented with 1.40µM IAA. The callus extract showed considerable DPPH radical scavenging, hydroxyl radical scavenging and ferrous ion chelation activity with IC50 value of 16.05, 170.7 and 29.16µg/mL respectively.

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Section: C B Amentioning

confidence: 62%

Effect of Indole -3- Acetic Acid on Callus Induction From Leaf Explant of Ocimum Basilicum Linn.

V.N¹,

Sirangeevi²,

Deeksha³

et al. 2015

krj

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“…The Ocimum genus represents a notably diverse and valuable medicinal plant, characterized by significant genetic variation, differing morphological traits [ 29 , 30 ], as well as variations in the content and composition of essential oils [ 29 , 31 , 32 ]. These aromatic essential oils and the nonvolatile compounds found in basil species are used in flavoring, fragrance, cosmetics, aromatherapy, and pharmaceuticals.…”

Section: Discussionmentioning

confidence: 99%

Phenotypic Variations and Bioactive Constituents among Selected Ocimum Species

Mulugeta,

Pluhár,

Radácsi

2023

Plants

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Basil (Ocimum species) represents an extraordinary group of aromatic plants that have gained considerable economic importance, primarily due to their essential oils, which have applications in medicine, culinary, and perfumery. The Ocimum genus encompasses more than 60 species of herbs and shrubs originally native to tropical regions. This genus stands out for its remarkable diversity, displaying a wide spectrum of variations in phenotype, chemical composition, and genetic makeup. In addition to genetic factors, the growth, development, and essential oil production of basil are also influenced by environmental conditions, ontogeny, and various other factors. Consequently, the primary objective of this study was to explore the diversity in both the morphological characteristics and essential oil composition among basil genotypes preserved within the gene bank of the Hungarian University of Agriculture and Life Sciences’ Department of Medicinal and Aromatic Plants. The investigation involved the assessment of fifteen basil genotypes, representing four distinct species: Ocimum basilicum (including ‘Anise’, ‘Clove’, ‘Fino Verde’, ‘Licorice’, ‘Mammoth’, ‘Mrs. Burns’, ‘Thai tömzsi’, ‘Thai hosszú’, and ‘Vietnamese basil’), Ocimum sanctum (green holy basils), Ocimum citrodora (Lemon basil), and Ocimum gratissimum (African and Vana holy basil). The genotypes exhibited significant variations in their morphological growth, essential oil content (EOC), and composition. African basil produced more biomass (408.3 g/plant) and showed robust growth. The sweet basil cultivars clove, licorice, Thai tömzsi, and Thai hosszú also exhibited similar robust growth trends. Vietnamese basil, on the other hand, displayed the lowest fresh biomass of 82.0 g per plant. Both holy basils showed EOC levels below 0.5%, while Mrihani basil stood out with the highest EOC of 1.7%. The predominant constituents of the essential oil among these genotypes comprised estragole, thymol, methyl cinnamate, linalool, and eugenol. In conclusion, this study showed that the genotypes of basil stored in the department’s gene bank exhibit a wide range of variability, both within and between species.

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“…RAPD is the most extensively used technique for the study of genetic diversity in plants especially in medicinal and aromatic plants including the genus Ocimum due to low cost, time saving, easy to handle, sequence information not required, comparative analysis are rapid, cover large genome area and gives high level of polymorphism [21]. Since Ocimum diversity study is very complex, researchers from different parts of the globe have studied the Ocimum diversity based on morphological variability, chemotaxonomy, molecular marker, and cytotaxonomy [6], [22], [23], [24], [25], [26], [27], [28], [29]. Ocimum species are varied across India, however a few reports are available about the Ocimum diversity study.…”

Section: Introductionmentioning

confidence: 99%

Diversity of the genus Ocimum (Lamiaceae) through morpho-molecular (RAPD) and chemical (GC–MS) analysis

Chowdhury

Mandal

Roy

et al. 2017

Journal of Genetic Engineering and Biotechnology

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In this present study, we have described the diversity of nine Ocimum genotypes naturally grown in the Dakshin Dinajpur district of West Bengal, India. Their diversity was determined on the basis of morphological, chemical and randomly amplified polymorphic DNA (RAPD) to determine the level of variation present in the genus Ocimum. Among nine Ocimum genotypes six (O. americanum, O. × africanum, O. basilicum, O. gratissimum, O. kilimandscharicum and O. tenuiflorum) are found to be different Ocimum species and the rest are as varieties. A total of 18 qualitative and 17 quantitative morphological traits and chemical compositions were evaluated. Significant variations were observed in the morphological traits except O. × africanum and O. basilicum species. Cluster generated from the morphological data showed two different groups viz. basilicum group and sanctum group. Chemical analysis did not show much variation between morphologically similar species viz. O. × africanum and O. basilicum. However, RAPD analyses clearly showed that O. × africanum and O. basilicum are different species. Thus the combined analyses of morphological traits, chemical and molecular markers represent the best possible approach to confirm taxonomic delineation. Moreover, we are reporting O. × africanum for the first time from this region as well as from West Bengal, India.

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Morphological-, chemical- and RAPD-PCR evaluation of eight different Ocimum basilicum L. gene bank accessions

Cited by 8 publications

References 16 publications

Effect of Indole -3- Acetic Acid on Callus Induction From Leaf Explant of Ocimum Basilicum Linn.

Effect of Indole -3- Acetic Acid on Callus Induction From Leaf Explant of Ocimum Basilicum Linn.

Phenotypic Variations and Bioactive Constituents among Selected Ocimum Species

Diversity of the genus Ocimum (Lamiaceae) through morpho-molecular (RAPD) and chemical (GC–MS) analysis

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