Morphological, Chemical and Functional Analysis of Catuaba Preparations

Abstract

Fourteen commercial samples of the popular Brazilian aphrodisiac Catuaba specified as bark drugs of Anemopaegma, Erythroxylum and Trichilia species were examined for identity and purity. Only a minority of the examined Catuaba samples contained the crude drugs claimed on the labels. More than half of the products were adulterated with different crude drugs. The majority of the samples contained a bark originating from Trichilia catigua. The TLC fingerprints confirmed the heterogeneity, in 50% of the samples tropane alkaloids of various concentrations were detected. TLC and HPLC methods for separation and identification of the tropane alkaloids were developed and their analytical data (RF values, retention times, ESI-MS) given. The structure elucidation of the two main alkaloids, catuabine D and its hydroxymethyl derivative, is presented. The 1H- and 13C-NMR assignments of these alkaloids are discussed with regard to literature data. Neither aqueous nor methanolic extracts of the Trichilia catigua reference material nor alkaloid-enriched fractions of commercial samples showed any effect on the rabbit corpus cavernosum in an in vitro test.

Phenylpropanoid substituted flavan-3-ols from Trichilia catigua and their in vitro antioxidative activity

“…Marques 2 described the differences among the species known as "catuaba" and determined that T. catigua is the main species that is sold as "catuaba" in Brazil. The same conclusion was reached by Kletter et al 3 and Daolio et al 4 The bark of T. catigua has been traditionally used as a health and mental tonic, and especially as a sexual stimulant. 3,5 Some pharmacological studies of this bark have reported antimicrobial, trypanocidal, antinociceptive, vasorelaxant or dopamine-mediated antidepressantlike effects.…”

“…The same conclusion was reached by Kletter et al 3 and Daolio et al 4 The bark of T. catigua has been traditionally used as a health and mental tonic, and especially as a sexual stimulant. 3,5 Some pharmacological studies of this bark have reported antimicrobial, trypanocidal, antinociceptive, vasorelaxant or dopamine-mediated antidepressantlike effects. [5][6][7][8][9][10] The bark contains a high concentration of polyphenols (6.96 ± 0.11%; RSD 1.57%) including flavan-3-ols and phenylpropanoid derivatives, 5,11 as well as tropane alkaloids.…”

“…[5][6][7][8][9][10] The bark contains a high concentration of polyphenols (6.96 ± 0.11%; RSD 1.57%) including flavan-3-ols and phenylpropanoid derivatives, 5,11 as well as tropane alkaloids. 3 Other species, T. claussenii and T. lepidota, were evaluated and yielded sesquiterpenoids. 12 We further investigated the constituents of T. catigua bark and isolated nine polyphenolic constituents, including one new compound.…”

Análise farmacognóstica de amostras de drogas vegetais psicoativas comercializadas em Diadema

“…A confusão na identificação iniciou-se no início do século 20 com uma tese de doutorado e perdura até os dias atuais (DAOLIO et al, 2008;KLETTER et al, 2004;TABANCA et al, 2007). DAOLIO et al, 2008;KLETTER et al, 2004).…”

“…As análises, em conjunto, permitiram verificar que os quatro lotes de catuaba se tratavam da espécie T. catigua, fato que também foi anteriormente observado na literatura (BELTRAME et al, 2006;DAOLIO et al, 2008), apesar da 1ª edição da Farmacopéia (1929), revogada, oficializar órgãos subterrâneos de A. mirandum. Kletter et al (2004) realizaram um projeto de avaliação da qualidade de amostras comerciais de catuaba, onde foi possível confirmar a prevalência das cascas de caule de T. catigua dentre as DVs presentes no mercado. Entretanto, os cuidados com a qualidade do material estudado se mostrou precária, com contaminação e problemas nos rótulos e armazenamento, da mesma forma que demonstram os resultados presentes nesta dissertação.…”