Morphological Characterization of Spermatozoa of the Night Monkey

Nakazato, Chiho; Yoshizawa, Midori; Isobe, Keisuke; Kusakabe, Ken Takeshi; Kuraishi, Takeshi; Hattori, Satoshi; Matsumoto, Hiromichi; Fukui, Emiko; Kuwahata, A.; Ochi, Masanori; Kiso, Yasuo; Kai, Chieko

doi:10.1274/jmor.32.37

Cited by 9 publications

(18 citation statements)

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“…Ova were collected from both ovaries of the dead female monkey as previously reported [3]. Sperm were collected from the male monkey as described by Nakazato et al [2]. A portion of caudal epididymal sperm suspension was introduced into a drop of HEPES-buffered human tubal fluid (HTF) medium (m-HTF; Irvine Scientific) containing 10 % fetal bovine serum (FBS).…”

Section: Methodsmentioning

confidence: 99%

“…We have previously reported on the morphology and normal parameters of owl monkey sperm [2] and oocytes [3]. However, because the motility and viability of owl monkey sperm are very poor, i.e., similar to those in some cases of male infertility in humans [2], embryo production by conventional in vitro fertilization is difficult in owl monkeys. Therefore, we decided that intracytoplasmic sperm injection (ICSI) is required to produce embryos in vitro.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Intracytoplasmic sperm injection into oocytes matured in vitro and early embryonic development in the owl monkey (Aotus lemurinus)

Watanabe

Matsumoto

Nishi

et al. 2015

Reprod Medicine & Biology

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Intracytoplasmic sperm injection into oocytes matured in vitro and early embryonic development in the owl monkey (Aotus lemurinus)

Watanabe

Matsumoto

Nishi

et al. 2015

Reprod Medicine & Biology

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“…; Nakazato et al. ). Traditionally, sperm morphology analyses have been carried out using subjective diagnostic methods to classify amorphous sperm heads, midpieces and tails.…”

Section: Introductionmentioning

confidence: 96%

“…In mammals, including primate species, sperm pathologies are frequently associated with marked or subtle changes in sperm morphology (Chemes and Rawe 2003;Valle et al 2012). Sperm pathologies such as teratozoospermia are often observed in primates including humans (Platz et al 1980;Cui et al 1991;Baccetti et al 2002;Nakazato et al 2015). Traditionally, sperm morphology analyses have been carried out using subjective diagnostic methods to classify amorphous sperm heads, midpieces and tails.…”

Section: Introductionmentioning

confidence: 99%

Sperm Morphology Assessment in Captive Neotropical Primates

Swanson

Valle

Carvalho

et al. 2016

Reprod Domestic Animals

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The main objective of this study was to evaluate sperm morphology in four neotropical primate species to compare the sperm morphological traits and the sperm morphometric parameters as a basis for establishing normative sperm standards for each species. Data from 80 ejaculates collected from four primate species, Callithrix jacchus, Callimico goeldii, Alouatta caraya and Ateles geoffroyi, were analysed for detection of sperm morphological alterations using subjective World Health Organization (WHO-2010) standards and Sperm Deformity Index (SDI) criteria, objective computer-assisted sperm morphometry analysis (CASMA) and subpopulation sperm determination (SSD) methods. There were multiple differences (p < 0.01) observed among primate species in values obtained from WHO-2010, SDI, CASMA and SSD sperm analysis methods. In addition, multiple significant positive and negative correlations were observed between the sperm morphological traits (SDI, Sperm Deformity Index Head Defects, Sperm Deformity Index Midpiece Defects, Sperm Deformity Index Tail Defects, Normal Sperm, Head Defects, Midpiece Defects and Tail Defects) and the sperm morphometric parameters (SSD, Area (A), Perimeter (P), Length (L), Width (W), Ellipticity, Elongation and Rugosity) (p ≤ 0.046). In conclusion, our findings using different evaluation methods indicate that pronounced sperm morphological variation exists among these four neotropical primate species. Because of the strong relationship observed among morphological and morphometric parameters, these results suggest that application of objective analysis methods could substantially improve the reliability of comparative studies and help to establish valid normative sperm values for neotropical primates.

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“…In Primates, however, there is little information on sperm morphometry from an evolutionary point of view. In particular, this subject has been poorly studied in neotropical primates (new world primates or Platyrrhini), with only a few species studied to this date [2,9,11,17,18,19,20,21,22,23,24].…”

Section: Introductionmentioning

confidence: 99%

Sperm Morphology in Neotropical Primates

Steinberg

Sestelo²,

Ceballos³

et al. 2019

Animals

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Simple SummaryThe spermatozoon is a highly differentiated cell, whose morphology has been affected throughout evolution by selective forces such as the competition between the sperm of rival males (sperm competition) and the joint evolution of male and female reproductive tracts (coevolution). The study of its morphology is important when analyzing the relationships between different species. In this contribution we analyzed new specimens and produced a database with all the spermatozoa dimensions recorded to date, comprising 75 individuals from 20 species and 8 genera, representing 2 families of neotropical primates (Cebidae and Atelidae). After an evolutionary analysis, we observed two different trends for the Cebidae and Atelidae families. Narrower and shorter spermatozoa seem to be the ancestral (oldest) form for Cebidae, with an evolutionary trend toward spermatozoa with wider and larger heads in the derived (younger) species. In Atelidae, on the contrary, narrower heads are observed in the more derived groups. We analyzed these results in the context of sperm competition and mating systems in these groups. More studies are needed to improve our knowledge of the evolution of the spermatozoa in neotropical primates.AbstractThe morphological and morphometric characterization of spermatozoa has been used as a taxonomic and phylogenetic tool for different species of mammals. We evaluated and compared the sperm morphometry of five neotropical primate species: Alouatta caraya, Ateles belzebuth and Ateles chamek of family Atelidae; and Cebus cay (=Sapajus cay) and Cebus nigritus (=Sapajus nigritus) of family Cebidae. After the collection of semen samples, the following parameters were measured on 100 spermatozoa from each specimen: Head Length, Head Width, Acrosome Length, Midpiece Length, Midpiece Width and Tail Length. Considering the available literature on sperm morphometry, we gathered data of 75 individuals, from 20 species, 8 genera and 2 families. These data were superimposed on a phylogeny to infer the possible direction of evolutionary changes. Narrower and shorter spermatozoa seem to be the ancestral form for Cebidae, with a trend toward wider and larger heads in derived groups. The spermatozoa of Atelidae may show an increase in total length and midpiece length. Sperm heads would have become narrower in the more derived groups of Ateles. Sperm length may increase in the more derived species in both families. Our results are discussed in the context of sperm competition and sexual selection.

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Morphological Characterization of Spermatozoa of the Night Monkey

Cited by 9 publications

References 11 publications

Intracytoplasmic sperm injection into oocytes matured in vitro and early embryonic development in the owl monkey (Aotus lemurinus)

Intracytoplasmic sperm injection into oocytes matured in vitro and early embryonic development in the owl monkey (Aotus lemurinus)

Sperm Morphology Assessment in Captive Neotropical Primates

Sperm Morphology in Neotropical Primates

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