Morphological characterization and size of hemocytes in Anodonta cygnea

Salimi, Lida; Jamili, Shahla; Motalebi, A. A.; Eghtesadi-Araghi, Peyman; Rabbani, Mohammad; Rostami-Beshman, M.

doi:10.1016/j.jip.2009.03.003

Cited by 23 publications

(12 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The function of this cell type in bivalves is unknown although its morphology may be the result of a granulocyte going through degranulation. This cell type has been reported in the mussel Anodonta cygnea (Salimi et al, 2009) oysters S. kegaki, O. circumpicta, H. hyotis, C. gigas (Chang et al, 2005Hong et al, 2013), the cockle Cerastoderma edule (Russell-Pinto et al, 1994), and the clam Scapharca inaequivalvis (Holden et al, 1994). The serous cell is rounded and has a light brown to almost black coloration to it when viewed unstained (Cheng, 1981;Haszprunar, 1996).…”

Section: Introductionmentioning

confidence: 72%

“…For this reason, the number of bivalve species covered in the hemocyte literature has continued to increase over the years. Bivalve species recently added to this literature base include Callista chione (Matozzo and Bailo, 2015), Bathymodiolus japonicas, B. platifrons, B. septemdierum (Tame et al, 2015), S. kegaki, O. circumpicta, H. hyotis (Hong et al, 2013), and A. cygnea (Salimi et al, 2009). The current study contributes to this effort by classifying the hemocytes of E. directus, and providing a detailed morphology of them using both transmission electron microscopy (TEM) and light microscopy.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Morphological characterization via light and electron microscopy of Atlantic jackknife clam (E directus) hemocytes

Preziosi

Bowden

2016

Micron

View full text Add to dashboard Cite

The Atlantic jackknife clam, Ensis directus, is currently being researched as a potential species for aquaculture operations in Maine. The goal of this study was to describe the hemocytes of this species for the first time and provide a morphological classification scheme. We viewed hemocytes under light microscopy (using Hemacolor, neutral red, and Pappenheim's stains) as well as transmission electron microscopy (TEM). The 2 main types of hemocytes found were granulocytes and hyalinocytes (agranular cells). The granulocytes were subdivided into large and small granulocytes while the hyalinocytes were subdivided into large and small hyalinocytes. The large hemocytes had both a larger diameter and smaller nucleus to cell diameter ratio than their smaller counterparts. A rare cell type, the vesicular cell, was also observed and it possessed many vesicles but few or no granules. Using TEM, granulocytes were found to contain both electron-lucent and electron-dense granules of various sizes. These numerous granules were the only structures that took up the neutral red stain. Hyalinocytes had few of these granules relative to granulocytes. Large hyalinocytes had both various organelles and large vesicles in their abundant cytoplasm while small hyalinocytes had little room for organelles in their scant cytoplasm. Total hemocyte counts averaged 1.96×10(6) cells mL(-1) while differential hemocyte counts averaged 11% for small hyalinocytes, 12% for large hyalinocytes, 59% for small granulocytes, and 18% for large granulocytes. The results of this study provide a starting point for future studies on E. directus immune function.

show abstract

Section: Introductionmentioning

confidence: 72%

Section: Introductionmentioning

confidence: 99%

Morphological characterization via light and electron microscopy of Atlantic jackknife clam (E directus) hemocytes

Preziosi

Bowden

2016

Micron

View full text Add to dashboard Cite

show abstract

“…During FCIFA, in addition to positive granulocytes, 8 AE 2.3% of haemocytes with low FS and SS was recognised by MAb 6H7 showing they possessed epitope of granulocytes but had morphological features of hyalinocytes, indicating an inconsistence in morphology and antigenicity of haemocyte population, which was originated from different classification criteria used in immunological technique and flow cytometry [30,31].…”

Section: Discussionmentioning

confidence: 99%

Development of a monoclonal antibody specific to granulocytes and its application for variation of granulocytes in scallop Chlamys farreri after acute viral necrobiotic virus (AVNV) infection

Lin¹,

Xing²,

Sheng³

et al. 2011

Fish & Shellfish Immunology

View full text Add to dashboard Cite

“…This cephalopod organization is distinct from that of bivalves and gastropods, where most Hc studies reported several circulating Hc types derived from one or more cell precursors (e.g. [5,8,9,11,15,17,18,20,32,96,97]). Our results further support the presence of a single mature circulating cell type in the Hc population of cephalopods.…”

Section: Fcm Analysis 341 Fresh Hc Cytogrammentioning

confidence: 99%

“…Among Molluscaone of the most diverse groups of animals [3], studies of immunity have mostly focused on bivalves (e.g. [4][5][6][7][8][9][10][11][12][13][14]) and gastropods (e.g. [15][16][17][18][19][20]), while few studies have focused on cephalopods [21,22].…”

Section: Introductionmentioning

confidence: 99%

Hemocyte morphology and phagocytic activity in the common cuttlefish (Sepia officinalis)

Pabic

Goux

Guillamin

et al. 2014

Fish & Shellfish Immunology

View full text Add to dashboard Cite

Little is known about the immune system of cephalopods, in spite of their many highly derived characters within the molluscan clade, including a vertebrate-like high-pressure closed circulatory system. Further the economic importance of cephalopod fisheries, potential for aquaculture, and use as ecotoxicology models demand a thorough understanding of their immune system. In this study, we present a comprehensive characterization of hemocytes in the common cuttlefish Sepia officinalis. Cytological stainings, electron microscopy- and flow cytometry-observations highlight a single granulocyte population with various densities of eosinophilic granules and unstained vesicles. These hemocytes contain acid phosphatase-, lysozyme- and proPO system enzymes, and have high activity in bead phagocytosis assays. Interestingly, bead pre-incubation in plasma results in time-dependent aggregation perhaps resulting from hemocyanin-coating, and decrease in phagocytosis. This study provides the basis for understanding hemocyte-mediated immunity in the common cuttlefish, and essential background for future studies on cephalopod immunity.

show abstract

Morphological characterization and size of hemocytes in Anodonta cygnea

Cited by 23 publications

References 27 publications

Morphological characterization via light and electron microscopy of Atlantic jackknife clam (E directus) hemocytes

Morphological characterization via light and electron microscopy of Atlantic jackknife clam (E directus) hemocytes

Development of a monoclonal antibody specific to granulocytes and its application for variation of granulocytes in scallop Chlamys farreri after acute viral necrobiotic virus (AVNV) infection

Hemocyte morphology and phagocytic activity in the common cuttlefish (Sepia officinalis)

Contact Info

Product

Resources

About