Morphological characteristics of natural strains of certain species of basidiomycetes and biological analysis of antimicrobial activity under submerged cultural conditions

Dyakov, M. Yu.; Камзолкина, О. В.; Shtaer, O. V.; Bisko, Nina; Поединок, Н. Л.; Mikhailova, O. B.; Tikhonova, Olga V.; Tolstikhina, T. E.; Vasil’eva, B. F.; Efremenkova, Olga V.

doi:10.1134/s0026261711020044

Cited by 19 publications

(19 citation statements)

References 16 publications

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“…The ability of L. tigrinus to antagonize indigenous microorganisms might be due to its reported ability to produce antimicrobial compounds able to inhibit a wide range of bacteria and fungi [24,25]. The inoculum formulation, using milled maize stalks (MMS) as the carrier, might be another determinant for the successful colonization of L. tigrinus .…”

Section: Resultsmentioning

confidence: 99%

Bioaugmentation of a historically contaminated soil by polychlorinated biphenyls with Lentinus tigrinus

et al. 2012

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BackgroundSeveral species belonging to the ecological group of white-rot basidiomycetes are able to bring about the remediation of matrices contaminated by a large variety of anthropic organic pollutants. Among them, polychlorobiphenyls (PCBs) are characterized by a high recalcitrance due to both their low bioavailability and the inability of natural microbial communities to degrade them at significant rates and extents. Objective of this study was to assess the impact of a maize stalk-immobilized Lentinus tigrinus CBS 577.79 inoculant combined with soybean oil (SO), as a possible PCB-mobilizing agent, on the bioremediation and resident microbiota of an actual Aroclor 1260 historically contaminated soil under unsaturated solid-phase conditions.ResultsBest overall PCB depletions (33.6 ± 0.3%) and dechlorination (23.2 ± 1.3%) were found after 60 d incubation in the absence of SO where, however, the fungus appeared to exert adverse effects on both the growth of biphenyl- and chlorobenzoate-degrading bacteria and the abundance of genes coding for both biphenyl dioxygenase (bph) and catechol-2,3-dioxygenase. A significant (P < 0.001) linear inverse relationship between depletion yields and degree of chlorination was observed in both augmented and control microcosms in the absence of SO; conversely, this negative correlation was not evident in SO-amended microcosms where the additive inhibited the biodegradation of low chlorinated congeners. The presence of SO, in fact, resulted in lower abundances of both biphenyl-degrading bacteria and bph.ConclusionsThe PCB depletion extents obtained in the presence of L. tigrinus are by far higher than those reported in other remediation studies conducted under unsaturated solid phase conditions on actual site soils historically contaminated by Aroclor 1260. These results suggest that the bioaugmentation strategy with the maize stalk-immobilized mycelium of this species might be promising in the reclamation of PCB-contaminated soils. The addition of SO to matrices contaminated by technical PCB mixtures, such as Aroclor 1242 and Delor 103 and characterized by a large preponderance of low chlorinated congeners, might not be advisable.

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Section: Resultsmentioning

confidence: 99%

Bioaugmentation of a historically contaminated soil by polychlorinated biphenyls with Lentinus tigrinus

et al. 2012

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“…За даними Дьякова зі співавторами (Dyakov et al, 2011) культури P. aurivella, P. lenta, P. squarrosa при культивуванні на агаризованому пивному суслі були віднесені до видів з середніми темпами росту.…”

Section: результати та обговоренняunclassified

“…Вивчення культурально-морфологічних особливостей штамів макроміцетів на агаризованих живильних середовищах дає можливість виявити додаткові таксономічні характеристики грибної культури, а також підібрати оптимальні живильні середовища для культивування та збереження штамів у належному фізіологічному стані (Bisko et al, 2012(Bisko et al, , 2018Mykchaylova et al, 2019). Дані щодо швидкості радіального росту вегетативного міцелію та морфологічних особливостей міцеліальної колонії для більшості видів роду Pholiota відсутні або ці важливі ознаки вивчені недостатньо , як у P. adiposa, P. aurivella, P. nameko, P. squarrosa (Buchalo, 1988;Cho et al, 2003;Buchalo et al, 2009;Dyakov et al, 2011;Bisko et al, 2012;Gizaw, 2015;Badalyan, Gharibyan, 2017). Актуальність нашого дослідження пояснюється необхідністю доповнення існуючих даних (Regeda, Bisko, 2019).…”

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Cultural and morphological characteristics of the species of Pholiota (Strophariaceae, Basidiomycota) on agar nutrient media

Regeda¹,

Bisko²

2020

Ukr. Bot. J.

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“…Встановлено, що екстракти, отримані як з плодових тіл, так і міцеліальної маси даного виду, мають імуномодулюючий, онкостатичний і антиметастатичний ефекти (Woodward et al, 1993;Kwon et al, 2009;Yamamoto et al, 2009;Yoshikawa et al, 2010;Takashi, 2013). За умов глибинного і поверхневого культивування штами S. crispa здатні до біосинтезу антибіотиків і цитокінінів (Dyakov et al, 2011;Vedenicheva et al, 2016).…”

unclassified

“…За даними літератури (Dyakov et al, 2011), для S. crispa характерні краплеподібні секреторні клітини на поверхні гіф і утворення кристалів різноманітної форми (голчасті, зібрані в пучки, тетраедричні). В умовах нашого експерименту кристали не формувались, але було виявлено численні секреторні клітини на поверхні гіф (рис.…”

unclassified

Морфолого-культуральні властивості рідкісного лікарського гриба Sparassis crispa (Sparassidaceae, Polyporales)

Mykchaylova¹,

Gryganskyi²,

Lomberg³

et al. 2017

Ukr J Ecol

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A verification of Sparassis crispa (Wulfen) Fr. strains of the IBK Mushroom Culture Collection using molecular-genetic and cultural-morphological methods were done. For all S. crispa strains a complete determination of the nucleotide sequences of the internal transcribed spacer: ITS1, 5.8S and ITS2 regions of rRNA, as well as the partial determination of 18S and 28S sequences surrounding the ITS, was performed. As a result of the search in the gene bank, the S. crispa samples deposited there showed 99-98% identity with the sequences we received, thus confirming the species of the examined strains. On the basis of the sequences we received, investigated strains were registered in NCBI GenBank. To confirm the taxonomic affiliation of the strains we studied their cultural and morphological characteristics. Microstructures of vegetative mycelium by optical and scanning electron microscopy were investigated. In all S. crispa cultures we observed the hyphae with regular one-sided gapless clamp connections, numerous secretory cells on the surface of the hyphae, anastomoses, filamentous strands and films. We studied the growth rate and morphology of the strains on seven agar nutrient media. According to the radial growth rate of S. crispa cultures can be placed to the very slowly growing mushrooms, growth rate of 0,5−2,8 mm/day. We found the selective media such as malt agar with the addition of pine sawdust (SS) and larch sawdust (SM) as most favorable for the vegetative growth and generative stage for all strains, temperature of incubation was 26 ± 0.1 °C. For mycelial growth the critical temperature was 39 ± 0.1 °C for S. crispa strains 312, 314 and 40 ± 0.1 °C for the strains 304, 2004. We confirmed belonging of studied strains to S. сrispa species according to established morphological and cultural characteristics. This result coincided with the results of DNA typing. Thus, the obtained mycelium growth parameters on nutrient media, micro-and macromorphological characteristics can be used as additional taxonomic characteristics of S. сrispa culture in the vegetative stage of growth. The strain S. сrispa 314 may become a potential producer for new fungal biotechnologies in Ukraine in the near future and reintroduction of this species in the nature.

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Morphological characteristics of natural strains of certain species of basidiomycetes and biological analysis of antimicrobial activity under submerged cultural conditions

Cited by 19 publications

References 16 publications

Bioaugmentation of a historically contaminated soil by polychlorinated biphenyls with Lentinus tigrinus

Bioaugmentation of a historically contaminated soil by polychlorinated biphenyls with Lentinus tigrinus

Cultural and morphological characteristics of the species of Pholiota (Strophariaceae, Basidiomycota) on agar nutrient media

Морфолого-культуральні властивості рідкісного лікарського гриба Sparassis crispa (Sparassidaceae, Polyporales)

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