Morphological Changes in the Kidneys of Rats with Postischemic Acute Renal Failure after Intrarenal Administration of Fetal Mesenchymal Stem Cells from Human Bone Marrow

Abstract

Chronic experiments on outbred albino rats were performed to compare the dynamics of histological signs for postischemic renal injury (90-min thermal ischemia) after intraparenchymal injection of cultured fetal MSC from human bone marrow. Functional indexes of the ischemic kidney were predetermined. In the early period after ischemia (day 4), administration of human bone marrow MSC was followed by the increase in blood flow in the microcirculatory bed and decrease in the degree of alteration in renal tubules. An increase in the area of zones with histological signs for normal function of tubules was accompanied by the improvement of biochemical indexes for renal function. In the delayed period, a protective effect of cell therapy was manifested in the prevention of death of renal tubules. Mild calcification of the necrotic tubular epithelium served as a marker of this process. Human bone marrow MSC were labeled with the fluorescent probe Calcein. These cells migrated from the site of injection, spread in the interstitium, and retained viability for 7 days. During this period, some cells were incorporated into the lumen of renal tubules.

Tissue engineering and stem cells: Basic principles and applications in urology

“…In addition, intraparenchymal injection of MSC increased blood flow in the microcirculatory bed and decreased the degree of alteration in renal tubules. In the delayed period, a protective effect was shown in the prevention of renal tubular death 20 . These results were favorable and asserted the potential use of SC in the functional recovery of an ischemic kidney.…”

“…In the delayed period , a protective effect was shown in the prevention of renal tubular death. 20 These results were favorable and asserted the potential use of SC in the functional recovery of an ischemic kidney.…”

Stem Cells and Tissue Engineering Techniques

“…In un modello di ischemia renale acuta dello stesso animale, CS derivate da tessuto renale adulto hanno manifestato la capacità di differenziare sia in vitro che in vivo in cellule endoteliali e muscolari. Inoltre, iniezioni intraparenchimali di CS mesenchimali (CSM) hanno prodotto l'aumento del flusso sanguigno nella microcircolazione e diminuito il livello di alterazione dei tubuli renali (23,24). Anche i risultati ottenuti utilizzando le CS in modelli murini di insufficienza renale cronica sembrano promettenti.…”

Characterization of Monoamine Oxidases in Mesenchymal Stem Cells: Role in Hydrogen Peroxide Generation and Serotonin-Dependent Apoptosis

“…M esenchymal stem cells (MSCs) are progenitor cells that can be isolated from different tissues, in particular from bone marrow and adipose tissue [1][2][3]. Results obtained in our and other laboratories showed that direct injection of MSCs into the tissue is associated with a functional recovery of ischemic kidney [ 4 ] and heart [ 5 ]. In particular, MSCs protect against both chemical and ischemia-reperfusion damage and promote repair process in models of acute renal injury [ 6 , 7 ].…”