Morphological Changes in the Denervated Muscle

Gutmann, E; Zelená, Jiřina

doi:10.1007/978-1-4899-4854-0_3

Cited by 95 publications

(51 citation statements)

References 39 publications

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“…One muscle contains about 3000 fibres which are singly innervated (Gutmann & Zelena, 1962) and about 30 pmol neurogenic ACh (Table 1), hence about 10-2 pmol ACh per end-plate. According to estimates by Elmqvist & Quastel (1965) the average size of the presynaptic transmitter store in a single rat phrenic nerve terminal corresponds to 270,000 quanta.…”

Section: Discussionmentioning

confidence: 99%

“…For comparison similar studies were made on unpoisoned, surgically denervated or reinnervated muscles. The extensor digitorum longus (EDL) muscle ofyoung rats was R. L. POLAK, L. C. SELLIN AND S. THESLEFF chosen since it is of a reasonable size (40-50 mg) having about 3000 singly innervated muscle fibres (Gutmann & Zelena, 1962) and has been extensively used in electrophysiological studies on the effects of denervation-reinnervation (Sellin, Libelius, Lundquist, TAgerud & Thesleff, 1980) and of BoTx poisoning (Cull-Candy, Lundh & Thesleff, 1976). ACh determinations were made by pyrolysis-mass fragmentography as described by Polak & Molenaar (1979).…”

Section: Introductionmentioning

confidence: 99%

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Acetylcholine content and release in denervated or botulinum poisoned rat skeletal muscle

Polak

Sellin

Thesleff

1981

The Journal of Physiology

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3. Spontaneous release of ACh varied in normal innervated muscles between 40 and 100 fmol/min. In the presence of 25 mM-KCl the rate of release increased about fourfold. In BoTx poisoned muscles spontaneous release was reduced by up to 60 % of control and high potassium failed to accelerate the release at 2 d after poisoning and caused only a small increase at 8 d. Denervated muscles released ACh at a rate which was less than 20 % of control and it was not accelerated by high potassium.4. The results show that more than 90 % oftotal ACh in the innervated EDL muscle is present in the nerve and its terminals. The remaining ACh is apparently formed and stored in the muscle tissue. BoTx caused a larger reduction in ACh release than can be accounted for by assuming a selective blockade of quantal release of transmitter. It suggests that BoTx has an inhibitory effect also on non-quantal ACh release.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Acetylcholine content and release in denervated or botulinum poisoned rat skeletal muscle

Polak

Sellin

Thesleff

1981

The Journal of Physiology

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“…(J Histochem Cytochem 56:111-123, 2008) K E Y W O R D S rat soleus reinnervation notexin regeneration myosin heavy chain fiber type transition THE DIFFERENT TYPES of muscle fibers are highly dependent on their innervation pattern (Pette and Staron 1990;Pette and Vrbová 1992). Loss of innervation resulting from neuromuscular diseases or accidents leads to muscle inactivity, causing general morphological and physiological deterioration of the affected fibers (Gutmann and Zelena 1962;Borisov et al 2001). Although myofibers are able to recover from denervation atrophy after the nerve-muscle contacts have been reestablished, muscle function, even after successful surgical repair, often fails to recover completely (Mackinnon and Dellon 1988;Hare et al 1992;Kalliainen et al 2002).…”

mentioning

confidence: 99%

Regeneration of Reinnervated Rat Soleus Muscle Is Accompanied by Fiber Transition Toward a Faster Phenotype

Mendler

Pintér

Kiricsi

et al. 2007

J Histochem Cytochem.

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S U M M A R Y The functional recovery of skeletal muscles after peripheral nerve transection and microsurgical repair is generally incomplete. Several reinnervation abnormalities have been described even after nerve reconstruction surgery. Less is known, however, about the regenerative capacity of reinnervated muscles. Previously, we detected remarkable morphological and motor endplate alterations after inducing muscle necrosis and subsequent regeneration in the reinnervated rat soleus muscle. In the present study, we comparatively analyzed the morphometric properties of different fiber populations, as well as the expression pattern of myosin heavy chain isoforms at both immunohistochemical and mRNA levels in reinnervated versus reinnervated-regenerated muscles. A dramatic slow-tofast fiber type transition was found in reinnervated soleus, and a further change toward the fast phenotype was observed in reinnervated-regenerated muscles. These findings suggest that the (fast) pattern of reinnervation plays a dominant role in the specification of fiber phenotype during regeneration, which can contribute to the long-lasting functional impairment of the reinnervated muscle. Moreover, because the fast II fibers (and selectively, a certain population of the fast IIB fibers) showed better recovery than did the slow type I fibers, the faster phenotype of the reinnervated-regenerated muscle seems to be actively maintained by selective yet undefined cues. (J Histochem Cytochem 56:111-123, 2008)

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“…This condition is considered a 'pathologically induced lipid deposition' that according to Gutmann and Zelena (1962) expressed the concept that fat cells replace atrophied muscle tissue during the late stages of denervation atrophy.…”

Section: Discussionmentioning

confidence: 99%

Lipomatous muscular ‘dystrophy’ of Piedmontese cattle

Biasibetti

Amedeo

Brugiapaglia

et al. 2012

Animal

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Lipomatous myopathy is a degenerative muscle pathology characterized by the substitution of muscle cells with adipose tissue, sporadically reported in cattle, pigs, and rarely in sheep, horses and dogs. This study investigated the pathology of this myopathy in 40 muscle samples collected from regularly slaughtered Piedmontese cattle living in Piedmont region (Italy). None of the animals showed clinical signs of muscular disease. Muscle specimens were submitted to histological and enzymatic investigations. Gross pathology revealed a different grade of infiltration of adipose tissue, involving multiple or single muscles. The most affected regions were the ventral abdomen and the shoulders, especially the cutaneous muscles and the muscles of the thoracic group. Morphological staining revealed an infiltration of adipose tissue varying in distribution and severity, changes in muscle fibre size and increased number of fibres with centrally located nuclei, suggesting muscle degeneration-regeneration. Necrosis and nonsuppurative inflammatory cells were also seen. Furthermore, proliferation of connective tissue and non-specific myopathic changes were present. Chemical and physical characteristics of the affected tissue were also evaluated. The authors discuss about the aetiopathogenesis and classification of this muscle disorder whose histological lesions were similar to those reported in human dystrophies.

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Morphological Changes in the Denervated Muscle

Cited by 95 publications

References 39 publications

Acetylcholine content and release in denervated or botulinum poisoned rat skeletal muscle

Acetylcholine content and release in denervated or botulinum poisoned rat skeletal muscle

Regeneration of Reinnervated Rat Soleus Muscle Is Accompanied by Fiber Transition Toward a Faster Phenotype

Lipomatous muscular ‘dystrophy’ of Piedmontese cattle

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