Morphological changes in lymph nodes and spleen upon EAE induction in C57BL/6 mic

Abstract

Myelin oligodendrocyte glycoprotein (MOG) is a protein widely used in the induction of experimental autoimmune encephalomyelitis (EAE) for studying human multiple sclerosis (MS). In C57BL/6 female mice aged eight weeks, we administered subcutaneously MOG35-55 peptide in CFA (complete Freund?s adjuvant) along with pertussis vaccine injected intraperitoneally. We observed the sign of flaccid tail as early as thirteen days post-immunization in five of twelve animals. Hematoxylin and eosin staining of paraffin-embedded sections of lymph nodes and spleen revealed the presence of germinal centers in the immunized animals. In the control group of animals, lymphoid follicles without germinal centers were observed. Immunohistochemical staining of spleen sections revealed an expression of MHC II molecules in the EAE-induced group. We would like to point out that even though the clinical signs are mild, the morphological changes are apparent in the lymph nodes and spleen of MOG35-55-immunized mice.

Effect of Cyclophosphamide Treatment on Central and Effector Memory T Cells in Mice

“…9,15,16 Due to its cytotoxic and immunosuppressive effects, CP is commonly used against a broad spectrum of cancers, including lymphoma, chronic lymphocytic leukemia, soft tissue and osteogenic sarcoma, several solid tumors, and autoimmune diseases. [17][18][19][20] Besides clinical applications, CP is also widely used in experimental research to induce immunodeficiency in animal models. 11,21,22 CP has been used to study numerous immune processes related to cancer development, transplant tolerance/ rejection, and infections.…”

“…Due to its cytotoxic and immunosuppressive effects, CP is commonly used against a broad spectrum of cancers, including lymphoma, chronic lymphocytic leukemia, soft tissue and osteogenic sarcoma, several solid tumors, and autoimmune diseases. 17 –20…”

Vaccination and autoimmunity: Influenza vaccination and association with multiple sclerosis

“…Irla et al 42 proposed that MHCII expression by pDCs confers natural protection against EAE by stimulating the selective expansion of myelin-Ag-specific natural regulatory T cells in secondary lymphoid tissues. In our previous study we suggested that pDCs might be the one to make a connection between mild clinical signs expressed in the myelin oligodendrocyte glycoprotein (MOG) variant of EAE-induced mice and the expression of MHC class II molecules in the secondary lymphoid organs 43 . Activation of DCs leads to their maturation, that is, upgrades the expression of MHC class II as well as co-stimulatory molecules (CD80, CD86, CD40).…”