Morphological Changes in Blood Vessels Produced by Hyperosmotic Agents and Measured by Optical Coherence Tomography¶

Vargas, Gracie; Readinger, Allison; Dozier, Susan S.; Welch, Ashley J.

doi:10.1562/0031-8655(2003)077<0541:mcibvp>2.0.co;2

Cited by 70 publications

(81 citation statements)

References 16 publications

(18 reference statements)

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“…(1) (2) where MC 0 and MC t are the mean contrasts of the modulation transfer function (MTF) immediately after glycerol application and at the elapsed time t, respectively. The MC was calculated as follows: 1. column pixels of the extracted image were added and their mean values calculated to obtain the MTF shown in Fig.…”

Section: Quantitative Evaluation Of Optical Skin Clearing Efficacymentioning

confidence: 99%

Enhancement of optical skin clearing efficacy using a microneedle roller

et al. 2008

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Light scattering in biological tissues can be reduced by using optical clearing agents. Various physical methods in conjunction with agents have been studied to enhance the optical clearing efficacy of skin for diagnostic and therapeutic applications. In this study, we propose a new physical method to enhance the optical clearing potential of topically applied glycerol. A microneedle roller is used to easily create numerous transdermal microchannels prior to glycerol application. The optical clearing efficacy of skin is quantitatively evaluated with the use of a modulation transfer function target placed underneath ex vivo porcine skin samples. From cross-polarized images acquired at various time points after glycerol application, we find that samples treated with the microneedle roller resulted in an approximately two-fold increase in contrast compared to control samples 30 min after glycerol application. In conclusion, our data suggest that the microneedle roller can be a good physical method to enhance transdermal delivery of optical clearing agents, and hence their optical clearing potential over large regions of skin.

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Section: Quantitative Evaluation Of Optical Skin Clearing Efficacymentioning

confidence: 99%

Enhancement of optical skin clearing efficacy using a microneedle roller

et al. 2008

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“…ear of nude mouse ∼270 µm thick), or (2) by decreasing light scattering using a recently developed optical clearing method combined with spectral selection (e.g. use a "green" filter to increase blood vessel contrast) [74][75][76][77] . Figure 1 illustrates our few attempts using these models and transmission microscopy to obtain high-resolution images of individual cells in blood flow without staining.…”

Section: Animal Modelsmentioning

confidence: 99%

Advances in small animal mesentery models for in vivo flow cytometry, dynamic microscopy, and drug screening

Galanzha¹

2007

WJG

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Using animal mesentery with intravital optical microscopy is a well-established experimental model for studying blood and lymph microcirculation in vivo . Recent advances in cell biology and optical techniques provide the basis for extending this model for new applications, which should generate significantly improved experimental data. This review summarizes the achievements in this specific area, including in vivo label-free blood and lymph photothermal flow cytometry, super-sensitive fluorescence image cytometry, light scattering and speckle flow cytometry, microvessel dynamic microscopy, infrared (IR) angiography, and high-speed imaging of individual cells in fast flow. The capabilities of these techniques, using the rat mesentery model, were demonstrated in various studies; e.g., real-time quantitative detection of circulating and migrating individual blood and cancer cells, studies on vascular dynamics with a focus on lymphatics under normal conditions and under different interventions (e.g. lasers, drugs, nicotine), assessment of lymphatic disturbances from experimental lymphedema, monitoring cell traffic between blood and lymph systems, and high-speed imaging of cell transient deformability in flow. In particular, the obtained results demonstrated that individual cell transportation in living organisms depends on cell type (e.g., normal blood or leukemic cells), the cell's functional state (e.g., live, apoptotic, or necrotic), and the functional status of the organism. Possible future applications, including in vivo early diagnosis and prevention of disease, monitoring immune response and apoptosis, chemo-and radio-sensitivity tests, and drug screening, are also discussed. TOPIC HIGHLIGHT

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“…Hence, hypodermic injection became a common method to improve optical clearing of skin in vivo, but it has always been associated with some degree of scathe to skin or even the blockage of blood vessels. 21,22 Therefore, previous studies on monitoring skin blood flow with LSCI were still based on an invasive dorsal skin flap window model.…”

Section: Introductionmentioning

confidence: 99%

Imaging dermal blood flow through the intact rat skin with an optical clearing method

et al. 2010

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Abstract. Noninvasive detection of skin microcirculation is very significant for clinical diagnosis and therapy of peripheral vascular disease. In this study, an optical clearing method enables a laser speckle contrast imaging ͑LSCI͒ technique to image dermal blood flow through intact rat skin. The optical clearing effect of rat skin in vitro caused by a mixture of PEG-400 and Thiazone is evaluated by a commercially available spectrophotometer with an integrating sphere. A LSCI system is applied to image the dermal blood flow of in vivo rat skin after topical treatment of the agents. The results show that the reduced scattering coefficient ͑630 nm͒ is down to 60% of the initial value after 40 min of topical treatment of the mixture on skin in vitro. In vivo experimental results show that a 12-min treatment of the mixture on the epidermis can make the skin transparent and dermal vessels can be observed, while treatment with pure PEG-400 cannot. The process of skin optical clearing lasts, and the skin can be recovered by treatment of saline solution. Because optical clearing decreases the temporal contrast, dermal blood flow can be obtained by the laser speckle contrast imaging technique. © 2010 Society of Photo-Optical Instrumentation Engineers.

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Morphological Changes in Blood Vessels Produced by Hyperosmotic Agents and Measured by Optical Coherence Tomography¶

Cited by 70 publications

References 16 publications

Enhancement of optical skin clearing efficacy using a microneedle roller

Enhancement of optical skin clearing efficacy using a microneedle roller

Advances in small animal mesentery models for in vivo flow cytometry, dynamic microscopy, and drug screening

Imaging dermal blood flow through the intact rat skin with an optical clearing method

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