Morphological changes characteristic of pregnancy in the uterine luminal epithelium during the preimplantation period in the mouse

Inamoto, Masahiro; Yamamura, Hideki

doi:10.2535/ofaj1936.60.2-3_87

Cited by 3 publications

(8 citation statements)

References 19 publications

(18 reference statements)

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“…From Day 2 to 3, the uterine lumen becomes very narrow, and vacuolated epithelial cells are rarely found [11]. It has been speculated that this change in the lumen and LE is a preliminary event necessary for the initiation of implantation [11]. On the other hand, there has been little information available about the mechanism of embryo spacing.…”

Section: Discussionmentioning

confidence: 99%

“…The restraint stress in this period induced disruption of normal alignment of the embryo along the MA axis, suggesting that the implantation site is decided by maternal factors. From Day 2 to 3, the uterine lumen becomes very narrow, and vacuolated epithelial cells are rarely found [11]. It has been speculated that this change in the lumen and LE is a preliminary event necessary for the initiation of implantation [11].…”

Section: Discussionmentioning

confidence: 99%

“…In the preimplantation stage, ciliated cells, which are supposed to have the function of transportation of the embryo, are rarely found among the LE [11]. It is possible that migration of the embryo in the uterus is regulated by physical action of the myometrium.…”

Section: Discussionmentioning

confidence: 99%

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Spacing of the Embryo in the Uterus is Disrupted by the Supine Position of the Body During the Peri-implantation Period in Mice

Sugiyama

Terakawa

Khan

et al. 2010

Journal of Reproduction and Development

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Abstract. This study aimed to clarify the mechanism of the spacing of murine embryos along the metrial and antimetrial (MA) axis of the uterus using our newly developed experimental model. The model mice were produced by keeping mice in the supine position from the pre-implantation to implantation period. The starting points and periods of restraint of the mice in the supine position were set variously during the peri-implantation. Then, the position of the embryo was evaluated morphologically. In only one group (set in the instrument from the second day of pregnancy, Day 2, to Day 5), strong disruption of embryo spacing along the MA axis was observed. On the other hand, there was little abnormality in embryo positioning in the groups that were treated from Day 3 to Day 5 or from Day 3 to Day 6. These results suggested that determination of the position of the embryo in the MA axis is not related to duration of the experiment (2 days or 3 days), but is related to the starting time-point of the experiment, at Day 2 or Day 3. In conclusion, the period between Days 2 and 3 is critical for determination of the position of the embryo along the MA axis. Key words: Embryo implantation, Gravity, Mice, Spacing (J. Reprod. Dev. 56: [191][192][193][194] 2010) mbryo implantation is one of the most important events in mammalian reproduction [1]. This implantation consists of two major phenomena; the first is positioning of the embryo in the uterus before apposition of the blastocyst to the uterine luminal epithelium (LE), and the second is initiation of direct feto-maternal communication after completion of positioning (above "apposition"). In polytocous species, such as the mouse, precise positioning of individual embryos in the right place is required before the implantation window opens. The spacing of the mouse embryo is defined as follows: (1) the embryo aligns along the long axis of the uterine horn (LA axis) in equally spaced manner and (2) the embryo is localized exclusively at the anti-metrial side of the uterine horn the metrial and anti-metrial axis (MA axis).In mice, fertilization occurs at the ampulla of the oviduct. Fertilized eggs migrate to the uterine horn during the 2-to 16-cell stage at Day 2 of pregnancy (the day of the vaginal plug is defined as the first day of pregnancy, Day 1). The morula moves to the uterus around noon on Day 3. Then, the embryo develops to the blastocyst stage, and it completes spacing in the morning on Day 4. Finally, the blastocyst begins to attach to the LE [1][2][3].Leukemia inhibitory factor (LIF) is an essential factor for initiation of pregnancy after positioning of the embryo in mice [4]. LIF is involved in the process of implantation; it is involved in apoptosis of the LE and successive decidualization. The molecular pathway of LIF leading to successful implantation has gradually been revealed. In addition to LIF, some molecules have been suggested to be indispensable factors for the progress of implantation [4][5][6]. On the other hand, the mechanisms of embryo spacing ...

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Spacing of the Embryo in the Uterus is Disrupted by the Supine Position of the Body During the Peri-implantation Period in Mice

Sugiyama

Terakawa

Khan

et al. 2010

Journal of Reproduction and Development

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“…This fact suggests that the preimplantation embryonic development is affected by alterations of the maternal hormonal balance. A delay in hormone-dependent changes leading to the formation of a 'receptive' endometrium was observed in the uterine luminal epithelium in mice receiving serial S.C. injection of haloperidol (Inamoto, 1983). Although the mouse ovum has the ability to develop autonomously as shown in in vitro culture, a mechanism to maintain the synchronization of ovum development and gestational change of reproductive organs might be present, and the mechanism might operate even under some abnormal conditions.…”

Section: Delay In Cleavage and Blastocyst Formationmentioning

confidence: 99%

“…The uterus undergoes a series of changes leading to what is called a 'receptive' phase of implantation (Psychoyos, 1973). Morphological changes throughout the preimplantation period have been observed in the rat endometrium (Lobe1 et al, 1967) and mouse uterine luminal epithelium (Inamoto and Yamamura, 1983).…”

Section: Delay In Gestational Changes In the Uterine Epitheliummentioning

confidence: 99%

Delay in Embryonic Development, Uterine Epithelial Changes and Implantation due to Maternal Treatment during Preimplantation Period*

Yamamura

Inamoto

Fukui

1984

Congenital Anomalies

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The hormonal requirements for ovum implantation vary among species; at least in the mouse and rat, progesterone and estrogen, of which production and secretion are controlled along the hypothalamo‐pituitary‐ovarian axis, are indispensable for the initiation of an implantation. In fact, implantation of the blastocyst in these species is delayed or prevented by not only ovariectomy, but also by: hypophysectomy, lesion of the median eminence or a treatment of the maternal organism with an agent that interferes with this endocrine control system. The preimplantation developmental events such as cleavage, blastocyst formation and shedding of the zona pellucida and gestational changes of the uterine luminal epithelium are delayed under a few conditions causing a delayed implantation. Experimental data suggesting that uterine changes leading to the formation of a ‘receptive’ endometrium are hormone‐dependent are accumulating. Thus, the observed delay in changes in the uterine epithelium may be attributed to the alteration of the endocrine control. In contrast, it is difficult to determine how the delay in embryonic development occurs. Among the conceivable explanations are: the direct effect on embryos (ova) of implantation‐delaying factors, a mechanism synchronizing embryonic development with the uterine change or disturbance of possible hormonal control over the embryonic development.

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Ultrastructure of the Uterotubal Junction in Preovulatory Pigs

Rodriguez‐Martinez

Nicander²,

Viring

et al. 1990

Anat Histol Embryol

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The ultrastructure of the surface epithelia from the uterotubal junction (UTJ), and the adjacent tubal isthmic and endometrial regions, was studied in preovulatory oestrus gilts, either unmated or inseminated 12 h before with fresh boar semen. The simple columnar epithelium of the UTJ consisted of non-ciliated (secretory) and ciliated cells. Secretory vesicles occurred in the secretory cells, especially in inseminated gilts. Lymphocytes, monocytes and macrophages were found dispersed basally among the epithelial cells. Phagocytosis of epithelial cells undergoing apoptosis was seen throughout the UTJ at oestrus, increasing after insemination. Neutrophilic granulocytes were found in the lamina propria of the uterine component of the UTJ, but only occasionally in the epithelium. After insemination, neutrophils invaded the uterine epithelium, to actively participate in intraepithelial phagocytosis or move into the lumen, engulfing spermatozoa. Neutrophils were absent from the UTJ proper and the isthmic epithelium, irrespective of the presence of spermatozoa in the lumen. Those spermatozoa in the uterine lumen that escaped phagocytosis had severely damaged plasma membranes, whereas those in the UTJ proper--concentrated towards the deep furrows of the diverticulae--mostly showed normal sperm ultrastructure.

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Morphological changes characteristic of pregnancy in the uterine luminal epithelium during the preimplantation period in the mouse

Abstract: Morphological changes characteristic of pregnancy in the uterine luminal epithelium during the preimplantation period in the mouse

Cited by 3 publications

References 19 publications

Spacing of the Embryo in the Uterus is Disrupted by the Supine Position of the Body During the Peri-implantation Period in Mice

Spacing of the Embryo in the Uterus is Disrupted by the Supine Position of the Body During the Peri-implantation Period in Mice

Delay in Embryonic Development, Uterine Epithelial Changes and Implantation due to Maternal Treatment during Preimplantation Period*

Ultrastructure of the Uterotubal Junction in Preovulatory Pigs

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