Morphological changes and regulation of the genes dmrt1 and cyp11b during the sex differentiation of barramundi (Lates calcarifer Bloch)

Banh, Quyen Q.; Domingos, Jose A.; Zenger, Kyall R.; Jerry, Dean R.

doi:10.1016/j.aquaculture.2017.05.022

Cited by 18 publications

(15 citation statements)

References 63 publications

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“…The expression level of dmrt1 mRNA was kept relatively stable from 35dph to 50 dph, except for 45 dph, and showed a sudden increase at 55 dph, then a sharp decrease at 60 dph and an increased again at 65 dph and decrease at 70 dph ( Figure 7). Similar results have been observed in some species such as O. aurea (Cao et al, 2007), and barramundi (Latescalcarifer; Banh, Domingos, Zenger, & Jerry, 2017). The dmrt1gene was detected exclusively to be expressed in Sertoli cells by in situ hybridization.…”

supporting

confidence: 86%

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Molecular cloning and expression analysis of the dmrt1 gene from discus fish ( Symphysodon aequifasciatus )

et al. 2019

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supporting

confidence: 86%

“…The dmrt1 expression was decreased to lowest at 60 dph. Similar result is also observed in barramundi L. carifer (Banh et al, ). KaoVillalon, Ribeiro, and Berger () found that reduced oestrogen synthesis causes a delay in Sertoli cell maturation in boars.…”

supporting

confidence: 86%

Molecular cloning and expression analysis of the dmrt1 gene from discus fish ( Symphysodon aequifasciatus )

et al. 2019

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“…Barramundi, also known as Asian seabass ( Lates calcarifer ), is a large protandrous hermaphrodite of commercial importance for fisheries and aquaculture in Southeast Asia and Australia, with production increasingly globally [ 20 ]. In barramundi, testicular tissues develop from an undifferentiated gonad during the second to sixth month of age, with spermatozoa released in the efferent ducts as early as four months of age [ 21 ]. Males are fully mature at about 3–4 years and sex change into female between 4–8 years of age [ 21 ], although this change can occur as early as 2–3 years of age in captive situations [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

“…In barramundi, testicular tissues develop from an undifferentiated gonad during the second to sixth month of age, with spermatozoa released in the efferent ducts as early as four months of age [ 21 ]. Males are fully mature at about 3–4 years and sex change into female between 4–8 years of age [ 21 ], although this change can occur as early as 2–3 years of age in captive situations [ 22 ]. Barramundi do not possess an ovotestis gonad, with the ovarian lumen forming de novo through profound morphological and histological changes in which testicular tissue degenerates within the solid lobes of the testes and ovarian tissue arises from ventral regions [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Sex-specific dmrt1 and cyp19a1 methylation and alternative splicing in gonads of the protandrous hermaphrodite barramundi

et al. 2018

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Epigenetics is involved in sex differentiation of gonochoristic and hermaphroditic fish species, whereby two genes dmrt1 (pro-male) and cyp19a1 (pro-female) are known to play major roles. Barramundi, Lates calcarifer, is an important tropical aquaculture species that undergo natural and permanent male to female sex change, a process for which the exact underlying molecular mechanisms are still unknown. To elucidate whether DNA methylation is involved in sex control of barramundi, a next-generation bisulfite amplicon sequencing approach was used to target 146 CpG sites within proximal promoters and first exons of seven sex-related genes (dmrt1, cyp19a1, amh, foxl2, nr5a2, sox8 and sox9) of 24 testis and 18 ovaries of captive and wild adult barramundi. Moreover, comparative expression profiles of the key dmrt1 and cyp19a1 genes were further investigated using RT-qPCR and Sanger sequencing approaches, whereas expression levels of remaining targeted genes were based on available literature for the species. Results showed that cyp19a1 and amh were more methylated in males, whereas dmrt1 and nr5a2 were more methylated in females (P < 0.001), with no gender differences found for foxl2, sox8 or sox9 genes (P > 0.05). Sex-biased promoter DNA methylation was inversely related to gene expression only for dmrt1 and nr5a2, and directly related to amh expression, whereas no differences in cyp19a1 expression were found between testes and ovaries. Notably, unique sex-specific alternative splicing of dmrt1 and cyp19a1 were discovered, whereby males lacked the full-length aromatase coding cyp19a1 mRNA due to partial or total exon splicing, and females lacked the dmrt1 exon containing the DM-domain sequence. This study advances the current knowledge aiming to elucidate the genetic mechanisms within male and female gonads of this large protandrous hermaphrodite by providing the first evidence of epigenetics and alternative splicing simultaneously affecting key genes (cyp19a1 and dmrt1) central to sex differentiation pathways.

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“…Gradients of gonadal differentiation or development such as observed in pejerrey have been reported in other teleost species including Coptodon ( Tilapia ) zillii [Yoshikawa and Oguri, 1978], Oryzias latipes Oguri, 1979, 1981], and Lates calcarifer [Banh et al, 2017]. However, the role(s) of such gradients and their molecular basis remain largely unknown.…”

Section: Discussionmentioning

confidence: 98%

Spatiotemporal Correlations between amh and cyp19a1a Transcript Expression and Apoptosis during Gonadal Sex Differentiation of Pejerrey, Odontesthes bonariensis

Sarida

Zhang

Yamamoto

et al. 2019

Sex Dev

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Sex determination in pejerrey is genetically prescribed by the Y chromosome-linked anti-müllerian hormone amhy but is also strongly influenced by water temperature during the critical period of sex determination. Its gonadal differentiation is characterized by a cephalocaudal and left-to-right histological gradient in both sexes that presumably helps prevent discrepant intersex development in different regions of the gonads in response to ambiguous thermal and genetic stimuli, but the relation of this gradient to molecular processes of sex differentiation is unknown. In this study, we investigated the spatiotemporal expression patterns of amh, gonadal aromatase (cyp19a1a), and apoptosis in relation to the histological gradient in ovaries and testes at an intermediate, sexually neutral temperature. The location and timing of expression of amh, cyp19a1a, and apoptosis seemed to be highly coordinated with the time of gonadal sex differentiation and the histological gradient of gonadal sex differentiation. Apoptosis occurred predominantly in the anterior region of the right gonads and is surmised to be a process to delay differentiation in this area compared to the left gonad, possibly as a means to ensure uniform development in both gonads. Aromatase expression early during development was noted even in putative XY males, supporting the notion of primacy of female development in pejerrey gonads. Thus, apoptosis may be particularly important to prevent discrepant gonadal differentiation in XY individuals where genetic pro-male (amhy), pro-female (cyp19a1a), and thermal stimuli may antagonize.

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Morphological changes and regulation of the genes dmrt1 and cyp11b during the sex differentiation of barramundi (Lates calcarifer Bloch)

Cited by 18 publications

References 63 publications

Molecular cloning and expression analysis of the dmrt1 gene from discus fish ( Symphysodon aequifasciatus )

Molecular cloning and expression analysis of the dmrt1 gene from discus fish ( Symphysodon aequifasciatus )

Sex-specific dmrt1 and cyp19a1 methylation and alternative splicing in gonads of the protandrous hermaphrodite barramundi

Spatiotemporal Correlations between<b><i> amh</i></b> and <b><i>cyp19a1a</i></b> Transcript Expression and Apoptosis during Gonadal Sex Differentiation of Pejerrey, <b><i>Odontesthes bonariensis</i></b>

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