Morphological, biochemical and molecular characterisation of the first Italian <i>Candida africana</i> isolate

Romeo, Orazio; Criseo, Giuseppe

doi:10.1111/j.1439-0507.2008.01630.x

Cited by 33 publications

(42 citation statements)

References 12 publications

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“…Moreover, Al-Hedaithy and Fotedar (1) recovered and studied 25 atypical C. albicans strains from 21 specimens from female patients in Saudi Arabia. The majority of these clinical isolates showed a carbohydrate assimilation profile typical of the C. africana strains reported in other studies (2,17).…”

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confidence: 60%

Molecular Epidemiology of Candida albicans and Its Closely Related Yeasts Candida dubliniensis and Candida africana

Romeo

Criseo

2009

J Clin Microbiol

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We performed a molecular study to determine the occurrence of Candida albicans, Candida africana, and Candida dubliniensis in different clinical samples. The study provides new insights into the epidemiology of candidiasis in hospitalized patients in three hospitals in southern Italy. It also reports the first detailed epidemiological data concerning the occurrence of C. africana in clinical samples.

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confidence: 60%

Molecular Epidemiology of Candida albicans and Its Closely Related Yeasts Candida dubliniensis and Candida africana

Romeo

Criseo

2009

J Clin Microbiol

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“…Furthermore, with the advent of molecular phylogenetic analysis, there have been several new species suggested as subdivisions of traditional species. For example, a recent study has suggested that the species Candida africana may be a separate species from C. albicans (41), but there is still uncertainty about this distinction (1,37). Similarly, recent work has shown that Candida parapsilosis groups II and III are actually separate species, Candida orthopsilosis and Candida metapsilosis, respectively (40), and that Cryptococcus neoformans has been described to have variants (25).…”

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confidence: 99%

Molecular Identification of Veterinary Yeast Isolates by Use of Sequence-Based Analysis of the D1/D2 Region of the Large Ribosomal Subunit

et al. 2010

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Conventional methods of yeast identification are often time-consuming and difficult; however, recent studies of sequence-based identification methods have shown promise. Additionally, little is known about the diversity of yeasts identified from various animal species in veterinary diagnostic laboratories. Therefore, in this study, we examined three methods of identification by using 109 yeast samples isolated during a 1-year period from veterinary clinical samples. Comparison of the three methods-traditional substrate assimilation, fatty acid profile analysis, and sequence-based analysis of the region spanning the D1 and D2 regions (D1/D2) of the large ribosomal subunit-showed that sequence analysis provided the highest percent identification among the three. Sequence analysis identified 87% of isolates to the species level, whereas substrate assimilation and fatty acid profile analysis identified only 54% and 47%, respectively. Less-stringent criteria for identification increased the percentage of isolates identified to 98% for sequence analysis, 62% for substrate assimilation, and 55% for fatty acid profile analysis. We also found that sequence analysis of the internal transcribed spacer 2 (ITS2) region provided further identification for 36% of yeast not identified to the species level by D1/D2 sequence analysis. Additionally, we identified a large variety of yeast from animal sources, with at least 30 different species among the isolates tested, and with the majority not belonging to the common Candida spp., such as C. albicans, C. glabrata, C. tropicalis, and the C. parapsilosis group. Thus, we determined that sequence analysis of the D1/D2 region was the best method for identification of the variety of yeasts found in a veterinary population.In both veterinary and human diagnostic laboratories, correct identification of yeasts is important for the care of patients. Traditionally, yeast identification has been performed using biochemical analysis, substrate assimilation methods, morphological examination, or various combinations of the three. To increase the ease of identification, commercial tests that use these methods have been created, but despite the convenience provided by these methods, identification of yeasts by these conventional applications can still be timeconsuming and difficult. In addition, considerable variability in the efficacy of these methods has been reported for identification of clinically important yeast (11; also reviewed in references 12, 32, 36, and 42), attributed primarily to the limitations of the databases used for the comparison of clinical isolates, as well as the subjectivity involved in the interpretation of results. Recent studies have also examined the effectiveness of various molecular identification methods for yeasts by the use of rRNA genes, with the internal transcribed spacer 1 (ITS1) and ITS2 regions and the region spanning the D1 and D2 regions (D1/ D2) shown to be the most useful for species-level identification of yeasts, as a result of the variability within...

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“…africana) (20), the taxonomic status of C. africana remains controversial. Although they are germ tube positive like C. albicans and C. dubliniensis, C. africana isolates studied to date have reportedly grown and produced hyphae more slowly than either C. albicans or C. dubliniensis and could be distinguished from both by an inability to assimilate several sugars or to produce chlamydospores and by appearance on chromogenic agars (18,19,(21)(22)(23). Moreover, despite an almost worldwide distribution, the overwhelming majority of C. africana isolates have been recovered from female genital specimens (reviewed in reference 16).…”

Section: T He Incidence Of Invasive Fungal Infections Caused By Unusualmentioning

confidence: 99%

Epidemiology, Antifungal Susceptibility, and Pathogenicity of Candida africana Isolates from the United Kingdom

Borman¹,

Székely²,

Linton³

et al. 2013

J Clin Microbiol

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Candida africana was previously proposed as a new species within the Candida albicans species complex, together with C. albicans and C. dubliniensis, although further phylogenetic analyses better support its status as an unusual variant within C. albicans. Here we show that C. africana can be distinguished from C. albicans and C. dubliniensis by pyrosequencing of a short region of ITS2, and we have evaluated its occurrence in clinical samples by pyrosequencing all presumptive isolates of C. albicans submitted to the Mycology Reference Laboratory over a 9-month period. The C. albicans complex constituted 826/1,839 (44.9%) of yeast isolates received over the study period and included 783 isolates of C. albicans, 28 isolates of C. dubliniensis, and 15 isolates of C. africana. In agreement with previous reports, C. africana was isolated exclusively from genital specimens, in women in the 18-to-35-year age group. Indeed, C. africana constituted 15/251 (6%) of "C. albicans" isolates from female genital specimens during the study period. C. africana isolates were germ tube positive, grew significantly more slowly than C. albicans and C. dubliniensis on conventional mycological media, could be distinguished from the other members of the C. albicans complex by appearance on chromogenic agar, and were incapable of forming chlamydospores. Here we present the detailed evaluation of epidemiological, phenotypic, and clinical features and antifungal susceptibility profiles of United Kingdom isolates of C. africana. Furthermore, we demonstrate that C. africana is significantly less pathogenic than C. albicans and C. dubliniensis in the Galleria mellonella insect systemic infection model.

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Morphological, biochemical and molecular characterisation of the first Italian Candida africana isolate

Cited by 33 publications

References 12 publications

Molecular Epidemiology of Candida albicans and Its Closely Related Yeasts Candida dubliniensis and Candida africana

Molecular Epidemiology of Candida albicans and Its Closely Related Yeasts Candida dubliniensis and Candida africana

Molecular Identification of Veterinary Yeast Isolates by Use of Sequence-Based Analysis of the D1/D2 Region of the Large Ribosomal Subunit

Epidemiology, Antifungal Susceptibility, and Pathogenicity of Candida africana Isolates from the United Kingdom

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