Morphological bactericidal fast-acting effects of peracetic acid, a high-level disinfectant, against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in tubing

Chino, Takashi; Nukui, Yoko; Morishita, Yoshiyuki; Moriya, Kyoji

doi:10.1186/s13756-017-0281-1

Cited by 26 publications

(13 citation statements)

References 30 publications

(30 reference statements)

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“…This indicates that PAA treatment 249 impacts the outer membrane of M. psychrotolerans cells and that the level of damage might depend on PAA concentration. Some studies revealed that the PAA treatment 251 generates cell surface irregularities and partial grooves, with the damage becoming 252 increasingly apparent as the sanitizer concentration is increased (Chino et al, 2017;253 Ujimine et al, 2017;Park et al, 2013;Mustapha and Liewen, 1989). We did not 254 observe any grave damage to M. psychrotolerans cell morphology after 20 ppm PAA 255 treatment.…”

Section: Sem Analysismentioning

confidence: 60%

Sanitizing efficacy and antimicrobial mechanism of peracetic acid against histamine-producing bacterium, Morganella psychrotolerans

Wang

Yamaki

Kawai

et al. 2020

LWT

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In this study, we aimed to investigate the sanitizing effects of peracetic acid (PAA), and the underlying mechanism of its antimicrobial action against Morganella psychrotolerans. The exposure of M. psychrotolerans to 20 ppm PAA for 5 min led to its decrease below the detection level, indicating that PAA has a significant antibacterial effect against M. psychrotolerans in vitro. Moreover, the viable counts of M. psychrotolerans on saury (Cololabis saira) surface were showed a reduction of 1.40 and 2.23 log CFU/cm 2 upon treatment with 80 ppm PAA for 1 and 5 min, 18 respectively. Further, PAA treatment delayed the growth of M. psychrotolerans on 19 saury surface during storage at 4°C. Next, the antimicrobial mechanism of PAA against M. psychrotolerans cells was investigated, and the damage to the cell membrane and cell surface upon PAA treatment was observed using scanning electron microscopy (SEM) and epifluorescence microscopy. The chromosomal DNA and the protein profiles after PAA treatment were also analyzed. Form our results, we hypothesized that the bactericidal effect of PAA treatment was mainly attributed to damage the bacterial cell membrane. These results indicate that PAA may be an efficient disinfectant against M. psychrotolerans and has applications in seafood processing and storage.

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Section: Sem Analysismentioning

confidence: 60%

Sanitizing efficacy and antimicrobial mechanism of peracetic acid against histamine-producing bacterium, Morganella psychrotolerans

Wang

Yamaki

Kawai

et al. 2020

LWT

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“…Guidelines and in vitro studies indicate potential differences in efficacy between detergents, [45][46][47][48][49] disinfectants, 48,50,51 and AERs. 52 In the current study, contamination was not shown to be dependent on reprocessing characteristics, which is in line with another multicenter study.…”

Section: Discussionmentioning

confidence: 99%

Nationwide risk analysis of duodenoscope and linear echoendoscope contamination

Rauwers

holt

Buijs

et al. 2020

Gastrointestinal Endoscopy

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Background and Aims: Contaminated duodenoscopes and linear echoendoscopes (DLEs) pose a risk for infectious outbreaks. To identify DLEs and reprocessing risk factors, we combined the data from the previously published nationwide cross-sectional PROCESS 1 study (Prevalence of contamination of complex endoscopes in the Netherlands) with the follow-up PROCESS 2 study. Methods: We invited all 74 Dutch DLE centers to sample !2 duodenoscopes during PROCESS 1, and all duodenoscopes as well as linear echoendoscopes during PROCESS 2. The studies took place 1 year after another. Local staff sampled each DLE at 6 sites according to uniform methods explained by online videos. We used 2 contamination definitions: (1) any microorganism with !20 colony-forming units (CFU)/20 mL (AM20) and (2) presence of microorganisms with GI or oral origin, independent of CFU count (MGOs). We assessed the factors of age and usage by performing an analysis of pooled data of both PROCESS studies; additional factors including reprocessing characteristics were only recorded in PROCESS 2. Results: Ninety-seven percent of all Dutch centers (72 of 74; PROCESS 1, 66; PROCESS 2, 61) participated in one of the studies, sampling 309 duodenoscopes and 64 linear echoendoscopes. In total, 54 (17%) duodenoscopes and 8 (13%) linear echoendoscopes were contaminated according to the AM20 definition. MGOs were detected on 47 (15%) duodenoscopes and 9 (14%) linear echoendoscopes. Contamination was not age or usage dependent (all P values !.27) and was not shown to differ between the reprocessing characteristics (all P values !.01). Conclusions: In these nationwide studies, we found that DLE contamination was independent of age and usage. These results suggest that old and heavily used DLEs, if maintained correctly, have a similar risk for contamination as new DLEs. The prevalence of MGO contamination of w15% was similarly high for duodenoscopes as for linear echoendoscopes, rendering patients undergoing ERCP and EUS at risk for transmission of microorganisms. (Gastrointest Endosc 2020;92:681-91.) Abbreviations: AER, automated endoscope reprocessor; AM20, any microorganism with !20 CFU/20 mL; CFU, colony-forming units; DLE, duodenoscope and linear echoendoscope; IFU, instructions for use; IQR, interquartile range; MGO, presence of microorganisms with GI or oral origin, independent of CFU count; PROCESS study, Prevalence of contamination of complex endoscopes in the Netherlands; SFERD, Dutch Steering Group for Flexible Endoscope Cleaning and Disinfection. DISCLOSURE: Dr Bruno has had the following relevant financial activities outside the submitted work: consultant for 3M; grant from 3M for an investigator-initiated study; consultant and lecturer for Boston Scientific; grants from Boston Scientific for investigator-initiated studies and industryinitiated studies; consultant and lecturer for Cook Medical; grants from Cook Medical for investigator-initiated studies and industry-initiated studies; grants from Pentax Medical. Dr Vos has had the following relevant fina...

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“…Moreover, S. aureus does not form spores or flagella, but possesses a capsule, can produce golden yellow pigment, and decompose mannitol (Tayeb-Fligelman et al, 2017). Additionally, it has also been found that tests of plasma coagulase, lactose fermentation and deoxyribonuclease are positive in S. aureus (Chino et al, 2017;Tayeb-Fligelman et al, 2017).…”

Section: Staphylococcus Aureusmentioning

confidence: 99%

Prevalence and Therapies of Antibiotic-Resistance in Staphylococcus aureus

Guo

Song

Sun

et al. 2020

Front. Cell. Infect. Microbiol.

521

350

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Infectious diseases are the second most important cause of human death worldwide; Staphylococcus aureus (S. aureus) is a very common human pathogenic microorganism that can trigger a variety of infectious diseases, such as skin and soft tissue infections, endocarditis, osteomyelitis, bacteremia, and lethal pneumonia. Moreover, according to the sensitivity to antibiotic drugs, S. aureus can be divided into methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA). In recent decades, due to the evolution of bacteria and the abuse of antibiotics, the drug resistance of S. aureus has gradually increased, the infection rate of MRSA has increased worldwide, and the clinical anti-infective treatment for MRSA has become more difficult. Accumulating evidence has demonstrated that the resistance mechanisms of S. aureus are very complex, especially for MRSA, which is resistant to many kinds of antibiotics. Therefore, understanding the drug resistance of MRSA in a timely manner and elucidating its drug resistance mechanism at the molecular level are of great significance for the treatment of S. aureus infection. A large number of researchers believe that analyzing the molecular characteristics of S. aureus can help provide a basis for designing effective prevention and treatment measures against hospital infections caused by S. aureus and further monitor the evolution of S. aureus. This paper reviews the research status of MSSA and MRSA, the detailed mechanisms of the intrinsic antibiotic resistance and the acquired antibiotic resistance, the advanced research on anti-MRSA antibiotics and novel therapeutic strategies for MRSA treatment.

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Morphological bactericidal fast-acting effects of peracetic acid, a high-level disinfectant, against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in tubing

Cited by 26 publications

References 30 publications

Sanitizing efficacy and antimicrobial mechanism of peracetic acid against histamine-producing bacterium, Morganella psychrotolerans

Sanitizing efficacy and antimicrobial mechanism of peracetic acid against histamine-producing bacterium, Morganella psychrotolerans

Nationwide risk analysis of duodenoscope and linear echoendoscope contamination

Prevalence and Therapies of Antibiotic-Resistance in Staphylococcus aureus

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