Morphological, Bacterial, and Secondary Metabolite Changes of Aplysina aerophoba upon Long-Term Maintenance Under Artificial Conditions

Gerçe, Berna; Voigt, Matthias; Rühle, Sebastian; Kirchen, Silke; Putz, Annika; Proksch, Peter; Obst, Ursula; Syldatk, Christoph; Hausmann, Rudolf

doi:10.1007/s00248-009-9560-6

Cited by 34 publications

(24 citation statements)

References 48 publications

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“…Few studies deal with the effects of aquarium keeping on sponge-associated bacteria. In Aplysina aerophoba, bacterial community composition appears to be very resilient upon environmental changes (Friedrich et al, 2001;Thoms et al, 2003;Gerçe et al, 2009). On the other hand, the diversity of bacteria associated with Mycale laxissima (Mohamed et al .…”

Section: Discussionmentioning

confidence: 98%

New phylogenetic lineages of the Spirochaetes phylum associated with Clathrina species (Porifera)

et al. 2010

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Though spirochetes have been repeatedly found in marine sponges and other invertebrates, little attention has been paid to the specificity of this association. This study demonstrates that different geno-and morphotypes of spirochetes can reside within the same sponge individual and develop in considerable numbers. Specimens of the calcareous sponge Clathrina clathrus collected from the Adriatic Sea off Rovinj (Croatia) were found to harbor spirochete-like bacteria, which were characterized by scanning electron microscopy (SEM), 16S rRNA gene analysis, and catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH). Two novel spirochete sequence types related to the Brachyspiraceae could be retrieved. By use of specifically designed CARD-FISH probes, the C. clathrus-associated sequences could be assigned to a linear and a helical spirochete morphotype. Both were located within the sponge mesohyl and resembled the spirochete-like cells identified by SEM. In addition, from a Clathrina sp., most likely C. coriacea, that originated from Indonesian coastal waters, four different spirochete type sequences were recovered. Two of these also affiliated with the Brachyspiraceae, the other two were found associated with the Spirochaetaceae, one with the genera Borrelia and Cristispira.

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Section: Discussionmentioning

confidence: 98%

New phylogenetic lineages of the Spirochaetes phylum associated with Clathrina species (Porifera)

et al. 2010

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“…At present, the relevance of culturing cyanobacteria derives from their ability to produce bioactive natural compounds for biotechnological application (Moore et al 1988;siVonen & börner 2008;gerçe et al 2009). Moreover, the chemical structures of over 13,000 novel compounds from marine organisms have been already determined and their potential use in pharmaceutics widely considered (FeniCal 1997).…”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of a new Halomicronema species (Cyanobacteria) isolated from the Mediterranean marine sponge Petrosia ficiformis (Porifera).

Caroppo¹,

Albertano²,

Bruno³

et al. 2012

Fottea

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A filamentous cyanobacterium (strain ITAC101) isolated from a Mediterranean sponge (Petrosia ficiformis) was characterized by a combined phenotypic and genetic approach. Morphological and ultrastructural observations were performed along with growth measurements and pigment characterization. The molecular phylogenetic analyses were based on the sequencing of the 16S rRNA gene. In culture conditions, strain ITAC101 is moderately halophilic and grew in the range 0.3-7.6% (w/v) salinity with the optimum at 3.6%. Cell dimensions, thylakoid arrangement and pigment composition of this cyanobacterium fit the Halomicronema genus description, and phylogenetic analyses evidenced 99.9% similarity with another strain endolithic in tropical corals. The new Halomicronema metazoicum species was established including the two cyanobacteria associated to marine animals.

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“…are hypothesized to be produced by the sponge, although the process may involve some collaboration from microbial constituents (Sacristán-Soriano et al, 2011a). Variability in both secondary metabolite production and the composition of Aplysina-associated bacterial communities has been observed across a variety of spatial and temporal scales (Friedrich et al, 2001;Gerçe et al, 2009;Sacristán-Soriano et al, 2011a, b). Fig.…”

Section: Discussionmentioning

confidence: 98%

Trade-Offs in Defensive Metabolite Production But Not Ecological Function in Healthy and Diseased Sponges

et al. 2012

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Diseases of marine organisms, and sponges in particular, are increasingly reported worldwide. Prior research indicates that the survival of sponges on reefs is due largely to their production of biologically active secondary metabolites that provide protection from a diversity of stressors. Aplysina Red Band Syndrome (ARBS) is an emerging disease affecting Caribbean rope sponges (Aplysina spp.), but it is not known whether secondary metabolites play a role in disease susceptibility and resistance. To investigate whether differences in secondary metabolites may explain variability in susceptibility to ARBS in Aplysina cauliformis, we used high performance liquid chromatography (HPLC) to generate chemical profiles from healthy tissue in both healthy and diseased sponges, and quantified peak areas for 15 metabolites. Analyses of healthy and diseased sponges revealed qualitative and quantitative differences in their chemical profiles. Aplysamine-1 and fistularin-3 were produced in significantly higher concentrations by healthy sponges, whereas aerothionin and 11-oxoaerothionin were found only in diseased sponges. At natural concentrations, extracts from both healthy and diseased sponges deterred feeding by an omnivorous reef fish. Fistularin-3 deterred feeding at concentrations found in healthy sponges, but not at concentrations found in diseased sponges. Aerothionin deterred feeding at concentrations found in diseased sponges, and may at least partially replace the loss of fistularin-3 as a feeding deterrent compound following pathogenesis, suggesting a trade-off in the production of feeding deterrent compounds. Extracts from healthy and diseased sponges inhibited bacterial growth, and both aplysamine-1 and fistularin-3 displayed selective antibacterial activity. Despite differences in secondary metabolite production between healthy and diseased sponges, the stress associated with ARBS does not appear to compromise the ability of A. cauliformis to maintain defenses against some of its natural enemies.

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Morphological, Bacterial, and Secondary Metabolite Changes of Aplysina aerophoba upon Long-Term Maintenance Under Artificial Conditions

Cited by 34 publications

References 48 publications

New phylogenetic lineages of the Spirochaetes phylum associated with Clathrina species (Porifera)

New phylogenetic lineages of the Spirochaetes phylum associated with Clathrina species (Porifera)

Identification and characterization of a new Halomicronema species (Cyanobacteria) isolated from the Mediterranean marine sponge Petrosia ficiformis (Porifera).

Trade-Offs in Defensive Metabolite Production But Not Ecological Function in Healthy and Diseased Sponges

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