Morphological assessment of the behavior of three‐dimensional anionic collagen matrices in bone regeneration in rats

Miguel, Fúlvio Borges; Cardoso, Ana Karina M.V.; Barbosa, Alynne da Silva; Marcantonio, Élcio; Góissis, Gilberto; Rosa, Fabiana Paim

doi:10.1002/jbm.b.30492

Cited by 23 publications

(38 citation statements)

References 20 publications

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“…Critical defects do not regenerate during the life span of the animal [12,26,[34][35][36]; thus they are a good model for the evaluation of the osteogenic potential of biomaterials designed to be used in bone regeneration. In our work we used 8 mm for the diameter of the defect in the calvaria of rat.…”

Section: Discussionmentioning

confidence: 99%

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Hydroxyapatite-alginate biocomposite promotes bone mineralization in different length scales in vivo

Paula

Barreto

Rocha-Leão

et al. 2009

Front. Mater. Sci. China

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Tissue engineering is a multidisciplinary research area that aims to develop new techniques and/or biomaterials for medical applications. The objective of the present study was to evaluate the osteogenic potential of a composite of hydroxyapatite and alginate in bone defects with critical sizes, surgically made in the calvaria region of rats. The rats (48 adult males), Rattus norvegicus Wistar, were divided into two groups: control (without composite implantation) and experimental (with composite implantation) and analyzed by optical microscopy at the biological time points 15, 45, 90 and 120 d, and transmission electron microscopy 120 d after the implantation of the biomaterial. It was observed that the biomaterial presented a high degree of fragmentation since the first experimental points studied, and that the fragments were surrounded by new bone after the duration of the project. These areas were studied by analytical transmission electron microscopy using an energy dispersive X-ray spectrometer. Three regions could be distinguished: (1) the biomaterial rich in hydroxyapatite; (2) a thin contiguous region containing phosphorus but without calcium; (3) a region of initial ossification containing mineralizing collagen fibrils with a calcium/phosphorus ratio smaller than the particles of the composite. The intermediate region (without calcium or containing very low amounts of calcium), which just surrounded the composite had not been described in the literature yet, and is probably associated specifically to the biocomposite used. The high performance of the biomaterial observed may be related to the fact that alginate molecules form highly anionic complexes and are capable of adsorbing important factors recognized by integrins from osteoblasts. Regions of fibrotic tissue were also observed mainly in the initial experimental points analyzed. However, it did not significantly influence the final result. In conclusion, the biomaterial presents a great potential for application as bone grafts in the clinical area.

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Section: Discussionmentioning

confidence: 99%

“…Progress in the tissue engineering area in the last decades made possible the improvement of regenerative techniques and the development of new techniques and biomaterials, particularly in the bone engineering area [26].…”

Section: Discussionmentioning

confidence: 99%

Hydroxyapatite-alginate biocomposite promotes bone mineralization in different length scales in vivo

Paula

Barreto

Rocha-Leão

et al. 2009

Front. Mater. Sci. China

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“…Each group of animals was divided into two subgroups for euthanasia at either 15 or 60 days postoperative, in a total amount of five animals/biomaterial/period of observation. These periods of observation were chosen since they represent the initial inflammatory response to the biomaterial implantation (15 days) and the consolidated aspect of the implanted region (60 days) 28. The area of the original surgical defect and the surrounding tissues were removed en bloc .…”

Section: Methodsmentioning

confidence: 99%

“…These periods of observation were chosen since they represent the initial inflammatory response to the biomaterial implantation (15 days) and the consolidated aspect of the implanted region (60 days). 28 The area of the original surgical defect and the surrounding tissues were removed en bloc. The blocks were fixed in 10% neutral formalin, rinsed with water and then decalcified in Morse's acid solution.…”

Section: Methodsmentioning

confidence: 99%

Histological evaluation of chitosan‐based biomaterials used for the correction of critical size defects in rat's calvaria

Spin‐Neto

Freitas

Pavone

et al. 2009

J Biomedical Materials Res

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Chitosan, a biopolymer obtained from chitin, and its derivates, such as chitosan hydrochloride, has been reported as wound healing accelerators and as possible bone substitutes for tissue engineering, and therefore these substances could be relevant in dentistry and periodontology. The purpose of this investigation was to make a histological evaluation of chitosan and chitosan hydrochloride biomaterials (gels) used in the correction of critical size bone defects made in rat's calvaria. Bone defects of 8 mm in diameter were surgically created in the calvaria of 50 Holtzman (Rattus norvegicus) rats and filled with blood clot (control), low molecular weight chitosan, high molecular weight chitosan, low molecular weight chitosan hydrochloride, and high molecular weight chitosan hydrochloride, numbering 10 animals, divided into two experimental periods (15 and 60 days), for each biomaterial. The histological evaluation was made based on the morphology of the new-formed tissues in defect's region, and the results indicated that there was no statistical difference between the groups when the new bone formation in the entire defect's area were compared (p > 0.05) and, except in the control groups, assorted degrees of inflammation could be seen. In conclusion, chitosan and chitosan hydrochloride biomaterials used in this study were not able to promote new bone formation in critical size defects made in rat's calvaria.

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“…Bone neoformation generally depends on the size and type of the defect [Takagi and Urist, 1982;Schmitz and Höllinger, 1986;Chesmel et al, 1998, Miguel et al, 2006, the species of the animal and the implant site. However, repairing defects of critical dimensions is still very difficult, because under these conditions, there is not sufficient formation or organization of blood clots [Frame, 1980], which is essential for bone regeneration.…”

Section: Introductionmentioning

confidence: 99%

Histomorphometric Analysis of Tissue Responses to Bioactive Glass Implants in Critical Defects in Rat Calvaria

Cardoso

Barbosa²,

Miguel³

et al. 2006

Cells Tissues Organs

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The aim of this study was to evaluate the osteogenic behavior of two chemically similar bioactive glass products (Biogran®and Perioglas®) implanted in critical bone defects in rat calvaria. Thirty-six transfixed bone defects of 8 mm diameter were made surgically in adult male Wistar rats. The animals were distributed equally into three groups: Biogran (GI), Perioglas (GII) and without implant material (control; GIII). The morphology and composition of both bioactive glasses were analyzed by scanning electron microscopy and energy-dispersive spectrometry. Tissue specimens were analyzed at the biological time points of 15, 30 and 60 days by optical microscopy and morphometry, demonstrating biocompatibility for the tested materials with moderate chronic inflammation involving their particles. Bone neoformation resulted only as a reparative reaction to an intentionally produced defect and was limited to the defect’s edges. No statistically significant differences among the groups were observed. At the scar interstice, abundant deposits of collagenous fibers enveloping the particles were noted. The present results indicated that the bioactive glasses, under the experimental conditions analyzed, did not show osteogenic behavior.

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Morphological assessment of the behavior of three‐dimensional anionic collagen matrices in bone regeneration in rats

Cited by 23 publications

References 20 publications

Hydroxyapatite-alginate biocomposite promotes bone mineralization in different length scales in vivo

Hydroxyapatite-alginate biocomposite promotes bone mineralization in different length scales in vivo

Histological evaluation of chitosan‐based biomaterials used for the correction of critical size defects in rat's calvaria

Histomorphometric Analysis of Tissue Responses to Bioactive Glass Implants in Critical Defects in Rat Calvaria

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