Morphological and quantitative studies of gland formation from inocula of monodispersed rat mammary cells

Gould, Michael N.; Biel, W.F.; Clifton, Kelly H.

doi:10.1016/0014-4827(77)90362-7

Cited by 50 publications

(22 citation statements)

References 3 publications

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“…Fig. 2B Ductal segments of human and rat mammary gland, also termed "mammary gland organoids" (22,23), can be isolated from excised mammary gland tissue by enzymatic dissociation of surrounding stroma. The isolated mammary organoids, as shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

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In vivo and in vitro gene transfer to mammalian somatic cells by particle bombardment.

Yang¹,

Burkholder²,

Roberts³

et al. 1990

Proc. Natl. Acad. Sci. U.S.A.

516

205

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Chimeric chloramphenicol acetyltransferase and P-galactosidase marker genes were coated onto fine gold particles and used to bombard a variety of mammalian tissues and cells. Transient expression of the genes was obtained in liver, skin, and muscle tissues of rat and mouse bombarded in vivo. Similar results were obtained with freshly isolated ductal segments of rat and human mammary glands and primary cultures derived from these explants. Gene transfer and transient expression were also observed in eight human cell culture lines, including cells of epithelial, endothelial, fibroblast, and lymphocyte origin. Using CHO and MCF-7 cell cultures as models, we obtained stable gene transfer at frequencies of 1

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Section: Resultsmentioning

confidence: 99%

“…Ductal segments of human and rat mammary gland were isolated from excised mammary tissues by collagenase and hyaluronidase digestion of surrounding stroma tissue (22,23). Mammary gland tissues and the derived cultures were generously provided by M. Gould (University of Wisconsin, Madison).…”

Section: Methodsmentioning

confidence: 99%

In vivo and in vitro gene transfer to mammalian somatic cells by particle bombardment.

Yang¹,

Burkholder²,

Roberts³

et al. 1990

Proc. Natl. Acad. Sci. U.S.A.

516

205

View full text Add to dashboard Cite

show abstract

“…and hyperplastic rodent mammary epithelial tissues and cells will grow in vivo when transplanted into the mammary and other fat pads, but not when transplanted subcutaneously [68,184,185]. Furthermore, when the embryonic mouse mammary gland is separated into mesenchyme and epithelium, the embryonic mesenchyme [186,187], particularly that component directly responsible for the fat pad [188], is still required for the normal growth and development of the mammary gland in vitro and in vivo.…”

Section: (10) Identification Of Growth Factors For Cultured Mammary Cmentioning

confidence: 99%

Stem cells and the development of mammary cancers in experimental rats and in humans

Rudland

1987

Cancer Metast Rev

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Evidence based on immunocytochemical staining and ultrastructure suggests that morphological gradations between epithelial and myoepithelial cells, and possibly between epithelial cells and alveolar-like cells occur in terminal ductal structures of rat and human mammary glands. The benign carcinogen-induced rat and benign human mammary tumors can contain epithelial, myoepithelial-like and alveolar-like cells, whereas the malignant counterparts mainly contain only epithelial-like cells. Clonal epithelial cell lines from normal rat mammary glands, benign tumors, and SV40-transformed human mammary glands can differentiate to either myoepithelial-like or alveolar-like cells. In those of the rat, the differentiation processes occur in steps: intermediate cells along the myoepithelial-like pathway resemble intermediates in terminal ductal structures in vivo, and can also generate certain well-differentiated mesenchymal elements of the original tumours. Differentiation of the benign rat cells to alveolar-like cells with mammatrophic hormones and retinoids in vitro leads to a reduction in their tumor-forming ability in vivo. Cell lines from malignant rat mammary tumors of increasing metastatic potential and from human ductal carcinomas largely fail to yield myoepithelial-like or alveolar-like cells and are relatively slow-growing. Growth of the rat mammary epithelial cells in culture is stimulated by a pituitary-derived mammatrophic growth factor (PMGF), prostaglandin E2, and alpha-transforming growth factor; the response of the malignant cell lines to PMGF is reduced. It is suggested that stem cells exist in the rat and human glands that are capable of differentiating to the other major cell types of the mammary parenchyma, and that during the carcinogenic process they generate genetically unstable cells which lose their ability to differentiate and attempt to maximise their intrinsically slow growth rate.

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“…An AD,0 is defined as the number of cells per injection required to produce at least one alveolar growth in 50% of the graft sites. This number can then be used to determine the frequency of clonogenic stem-like cells or the clonogenic fraction in the cell suspension (12,13). Clonogen survival following carcinogen exposure is estimated by dividing the value of the AD,0 of the control, nontreated group by the AD., of the treated group.…”

Section: Specific Aimsmentioning

confidence: 99%

Impulse Response of Alternative Synthetic Apertures for Subsurface Detection

Ralston¹

2001

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Morphological and quantitative studies of gland formation from inocula of monodispersed rat mammary cells

Cited by 50 publications

References 3 publications

In vivo and in vitro gene transfer to mammalian somatic cells by particle bombardment.

In vivo and in vitro gene transfer to mammalian somatic cells by particle bombardment.

Stem cells and the development of mammary cancers in experimental rats and in humans

Impulse Response of Alternative Synthetic Apertures for Subsurface Detection

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