Morphological and quantitative aspects of nodule formation in hemolymph of the blowfly Chrysomya megacephala (Fabricius, 1794)

Faraldo, A. C.; Gregório, Elisa Aparecida; Lello, Edy de

doi:10.1016/j.exppara.2007.09.009

Cited by 8 publications

(6 citation statements)

References 37 publications

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“…On day 14 post-infection, similar changes were observed for these haemocyte types, except that the proportion of GRs was significantly greater than the control. Similar results were obtained with different insect species when infected with different microorganisms (Faraldo et al, 2008;Cunha et al, 2009). …”

Section: B Thuringiensissupporting

confidence: 89%

“…Similar observations were reported by Brayner et al (2007) who found that GRs, PLs and OEs presented morphological alterations indicative of innate immunological activation in mosquitoes infected with W. bancrofti, while PRs did not show any ultrastructural alteration. Similarly, Faraldo et al (2008) reported that at 24-h post-injection of Saccharomyces cerevisae yeast cells to Chrysomya megacephala cell debris and some free yeast cells were surrounded by granules and electron dense PLs which were probably initiating the nodulation process.…”

Section: Ultrastructural Changes In a Ipsilon Haemocytesmentioning

confidence: 95%

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Changes in the haemocytes of Agrotis ipsilon larvae (Lepidoptera: Noctuidae) in relation to dimilin and Bacillus thuringiensis infections

El-Aziz

Awad

2010

Micron

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Section: B Thuringiensissupporting

confidence: 89%

Section: Ultrastructural Changes In a Ipsilon Haemocytesmentioning

confidence: 95%

Changes in the haemocytes of Agrotis ipsilon larvae (Lepidoptera: Noctuidae) in relation to dimilin and Bacillus thuringiensis infections

El-Aziz

Awad

2010

Micron

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“…It is known that Chrysomya megacephala (D.), can transmit several pathogenic microbes, but cannot be infected, thus providing a good model for an immunity study (Faraldo et al , 2008). Detailed research on its immunity mechanism may provide more recent evidence for immunology.…”

Section: Introductionmentioning

confidence: 99%

Eicosanoids mediate nodulation reactions to bacterial Escherichia coli K 12 infections in larvae of the oriental blowfly, Chrysomya megacephala

et al. 2009

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Nodulation is the predominant cellular defense reaction to bacterial challenges in insects. In this study, third instar larvae of Chrysomya megacephala were injected with bacteria, Escherichia coli K 12 (10 6 CFU/mL, 2 μL), immediately prior to injection of inhibitors of eicosanoid biosynthesis, which sharply reduced nodulation response. Test larvae were treated with specific inhibitors of phospholipase A 2 (dexamethasone), cyclooxygenase (indomethacin, ibuprofen and piroxicam), dual cyclo-oxygenase/lipoxygenase (phenidone) and lipoxygenase (esculetin) and these reduced nodulation except esculetin. The influence of bacteria was obvious within 2 h of injection (5 nodules/larva), and increased to a maximum after 8 h (with 15 nodules/larva), and then significantly reduced over 24 h (9 nodules/larva). The inhibitory influence of dexamethasone was apparent within 2 h of injection (4 vs. 5 nodules/larva), and nodulation was significantly reduced, compared to control, over 24 h (5 vs. 8 nodules/larva). Increased dosages of ibuprofen, indomethacin, piroxicam and phenidone led to decreased numbers of nodules. Nodules continued to exist during the pupal stage. However, the effects of dexamethasone were reversed by treating bacteria-injected insects with an eicosanoid-precursor polyunsaturated fatty acid, arachidonic acid. These findings approved our view that eicosanoid can mediate cellular defense mechanisms in response to bacterial infections in another Dipteran insect C. megacephala.

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“…Insect candidates for this kind of investigation are the larvae of blowfly ( Chrysomya megacephala ), which normally feed on carrions with massive microbial blooms. To survive, the larvae developed some defenses against these microbial invaders, including the rapid release of antimicrobial molecules in the haemolymph (Faraldo, Gregório, & Lello, ). Since they live in highly hostile habitats, the innate immune system of C. megacephala should be better than those of all other insects.…”

Section: Introductionmentioning

confidence: 99%

“…To date, few studies on antibacterial activity have been reported in the haemolymph and secretion from the larvae and pupae of C. megacephala (Chaiwong et al, ; Faraldo, et al, ; Mohamed, , ; Ratcliffe et al, ; Sahalan, Omar, Mohamed, & Jeffery, ). No attempt has been made to isolate and purify antibacterial molecules with lytic properties from C. megacephala .…”

Section: Introductionmentioning

confidence: 99%

A serine protease‐associated lectin in the cytolytic system of blowfly (Chrysomya megacephala) larvae: Evidence and characterization

Paulchamy

Bhuvaragavan

Hilda

et al. 2019

Arch Insect Biochem Physiol

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Cytolytic activity against invading microorganisms is one of the innate forms of immunity in invertebrates. A serine proteaseassociated sialic acid-specific cytolytic lectin was purified using glutaraldehyde-fixed ox erythrocytes from the larval extract of blowfly (Chrysomya megacephala). The purified lectin lysed vertebrate erythrocytes with effective haemolysis of ox red blood cells (RBCs) in an isotonic medium. The degree of haemolytic (HL) activity of the purified cytolytic lectin depended on its concentration, pH, temperature, and calcium ions. It was sensitive to ethylenediaminetetraacetic acid. The native molecular mass of the C-type lectin was 260 ± 26 kDa, comprising four different polypeptide subunits of 75 kDa (pI~8), 69 kDa (pI~7.0), 61 kDa (pI~5.3), and 55 kDa (pI~4.6). The association between the C-type lectin and serine protease was confirmed by MALDI-TOF-MS analysis that revealed its homology in the same spectral peak as well as the proteases and phenylmethylsulphonyl fluoride inhibition of HL activity. Haemolysis inhibition by N-acetylneuraminic acid and other sugars revealed the properties of the lectin. The purified lectin distorted the integrity of ox RBCs and Paenalcaligenes hermetiae. This in vitro study documents the presence of a cytolytic system in blowfly (C. megacephala) larvae for the clearance of invading microbial pathogens in their feeding niche.

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Morphological and quantitative aspects of nodule formation in hemolymph of the blowfly Chrysomya megacephala (Fabricius, 1794)

Cited by 8 publications

References 37 publications

Changes in the haemocytes of Agrotis ipsilon larvae (Lepidoptera: Noctuidae) in relation to dimilin and Bacillus thuringiensis infections

Changes in the haemocytes of Agrotis ipsilon larvae (Lepidoptera: Noctuidae) in relation to dimilin and Bacillus thuringiensis infections

Eicosanoids mediate nodulation reactions to bacterial Escherichia coli K 12 infections in larvae of the oriental blowfly, Chrysomya megacephala

A serine protease‐associated lectin in the cytolytic system of blowfly (Chrysomya megacephala) larvae: Evidence and characterization

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