Morphological and polymerase chain reaction-restriction fragment lenght polymorphism characterization of Biomphalaria kuhniana and Biomphalaria amazonica from Colombia

Velásquez, Luz Elena; Caldeira, Roberta Lima; Estrada, Victoria; Carvalho, Omar S.

doi:10.1590/s0074-02762002000700012

Cited by 7 publications

(4 citation statements)

References 22 publications

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“…Indeed using our results obtained by morphology, PCR-RFLP and phylogeny we confirm that some snails from Brazil (Mato Grosso and Amazonas) identified as B. amazonica were actually B. cousini. Such misclassification may also have occurred in Colombia since some populations of B. cousini previously identified as B. amazonica (Velasquez et al, 2002), were molecularly characterized as B. cousini in the present study.…”

Section: Discussionmentioning

confidence: 83%

“…To date, its distribution is restricted to the Brazilian states of Amazonas (Paraense, 1966), Acre, Rondônia (Paraense, 1983), Mato Grosso (Paraense and Corrêa, 1985), Mato Grosso do Sul (Dorval and da Silva, 1990), Bolivia (Pointier et al, 2002) and Colombia (Velasquez et al, 2002). Another species was described in the same publication, Biomphalaria cousini Paraense, 1966 (type locality: Santo Domingo de los Colorados de Pichincha, Ecuador), which is morphologically similar to B. amazonica, making then difficult to be distinguished.…”

Section: Introductionmentioning

confidence: 99%

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Occurrence of Biomphalaria cousini (Mollusca: Gastropoda) in Brazil and its susceptibility to Schistosoma mansoni (Platyhelminths: Trematoda)

Teodoro¹,

Janotti-Passos²,

Carvalho³

et al. 2010

Molecular Phylogenetics and Evolution

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In Brazil, there are three intermediate snail vectors and two potential hosts of Schistosoma mansoni. Previous studies showed three variant molecular profiles to B. amazonica and evidenced intraspecific variations using sequence data. In this context, the aim of this study was to verify whether such differences would correspond to either B. amazonica or B. cousini. The snails were morphologically identified; PCR-RFLP and sequencing were carried out. Besides, B. cousini were submitted to susceptibility experiments to S. mansoni. Noteworthy, morphological data of Brazilian specimens predominantly showed the morphology described for B. amazonica. Nevertheless, PCR-RFLP results exhibited three variant molecular profiles for the specimens previously identified as B. amazonica and the phylogenetic analyses showed two groups one to B. amazonica and another to B. cousini. Furthermore, B. cousini showed to be susceptible to S. mansoni. These results confirm the occurrence of B. cousini in Brazil and points to the risk of introduction of schistosomiasis mansoni into new areas.

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Section: Discussionmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Occurrence of Biomphalaria cousini (Mollusca: Gastropoda) in Brazil and its susceptibility to Schistosoma mansoni (Platyhelminths: Trematoda)

Teodoro¹,

Janotti-Passos²,

Carvalho³

et al. 2010

Molecular Phylogenetics and Evolution

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“…B. kuhniana snails were found in 27.8% (5/18) of the surveyed sites. Although this species is not thought to be important for the transmission of schistosomiasis, its close morphological similarity to the intermediate host B. straminea highlights the importance of correctly identifying these snail species in order to accurately map potential foci of schistosomiasis [ 48 – 50 ].…”

Section: Discussionmentioning

confidence: 99%

A loop-mediated isothermal amplification assay for Schistosoma mansoni detection in Biomphalaria spp. from schistosomiasis-endemic areas in Minas Gerais, Brazil

et al. 2021

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Background Schistosomiasis a neglected tropical disease endemic in Brazil. It is caused by the trematode Schistosoma mansoni, which is transmitted by snails of the genus Biomphalaria. Among measures used to control and eliminate schistosomiasis, accurate mapping and monitoring of snail breeding sites are recommended. Despite the limitations of parasitological methods, they are still used to identify infected snails. Loop-mediated isothermal amplification (LAMP) is a sensitive, rapid, and cost-effective diagnostic method for the identification of infected snails. In the work reported here, we aimed to validate the use of LAMP for the detection of S. mansoni in snails of the genus Biomphalaria. Methods Snails were collected in five municipalities of the Mucuri Valley and Jequitinhonha Valley regions in the state of Minas Gerais, Brazil. Snails were pooled according to collection site and then squeezed for the detection of S. mansoni and other trematode larvae. Pooled snails were subjected to pepsin digestion and DNA extraction. Molecular assays were performed for species-specific identification and characterization of the samples. A previously described LAMP assay was adapted, evaluated, and validated using laboratory and field samples. Results Using the parasitological method described here, S. mansoni cercariae were detected in snails from two collection sites, and cercariae of the family Spirorchiidae were found in snails from one site. The snails were identified by polymerase chain reaction (PCR)–restriction fragment length polymorphism (RFLP). Biomphalaria glabrata, the main snail host of S. mansoni in Brazil, was detected in 72.2% of the collection sites. Biomphalaria kuhniana, which is resistant to S. mansoni infection, was found in the remaining sites. Multiplex, low stringency (LS), and conventional PCR allowed the detection of positive snails in four additional sites. Trematodes belonging to the families Strigeidae and Echinostomatidae were detected by multiplex PCR in two sites. The LAMP assay was effective in detecting the presence of S. mansoni infection in laboratory (7 days post-infection) and field samples with no cross-reactivity for other trematodes. When compared to LS and conventional PCR, LAMP showed 100% specificity, 85.7% sensitivity, and a κ index of 0.88. Conclusions Our findings suggest that LAMP is a good alternative method for the detection and monitoring of transmission foci of S. mansoni, as it was three times as effective as the parasitological examination used here for the detection of infection, and is more directly applicable in the field than other molecular techniques. Graphical abstract

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“…In fact, convergent shell evolution among African Biomphalaria species has been detected, complicating species identification using conchological characters (Plam et al ., 2008). The incorporation of molecular techniques, based mainly on restriction fragment length polymorphism (RFLP) analyses, has helped in the identification and recognition of species, although most of the studies have concentrated on Neotropical species (Caldeira et al ., 1998, 2000; Vidigal et al ., 1998, 2000a, b, 2001, 2002; Spatz et al ., 1999, 2000; Velásquez et al ., 2002). At the time of writing, 30–37 species are considered valid within Biomphalaria (DeJong et al ., 2001; Jørgensen, Kristensen & Stothard, 2007; Caldeira, Teodoro & Carvalho, 2008).…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic relationships and taxonomy of Altiplano populations of Biomphalaria (Gastropoda: Planorbidae): inference from a multilocus approach

Collado

Méndez

2012

Zoological Journal of the Linnean Society

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Isolated closed basins provide a natural laboratory to study the differentiation among wild populations. Here we examined the phylogenetic relationships of the Southern Altiplano populations of Biomphalaria, a genus with medical importance, using nuclear (ITS1, ITS2) and mitochondrial (16S) ribosomal gene markers and a species of Helisoma as outgroup. Phylogenetic trees based on separate and combined analyses show that these populations form a particular lineage within Biomphalaria along with Biomphalaria peregrina (d'Orbigny, 1835) and Biomphalaria oligoza Paraense, 1974. The origin of this clade was estimated to have occurred in the middle Pleistocene. Molecular analyses showed that the nominal species Biomphalaria crequii (Courty, 1907) from the Salar de Ascotán and Biomphalaria costata (Biese, 1951) from the Salar de Carcote, previously synonymized with Biomphalaria andecola (d'Orbigny, 1835) and B. peregrina, respectively, are distinct taxa. Molecular data did not resolve the relationship of Biomphalaria aymara Valdovinos & Stuardo, 1991 from the Isluga swamps to other Biomphalaria species, but confirm that the populations from the Lauca and Huasco basins may represent a distinct undescribed species of Biomphalaria from the Southern Altiplano. Snails examined for trematodes were found to be positive in some Altiplano localities. © 2012 The Linnean Society of London, Zoological Journal of the Linnean Society, 2012, 165, 795–808.

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Morphological and polymerase chain reaction-restriction fragment lenght polymorphism characterization of Biomphalaria kuhniana and Biomphalaria amazonica from Colombia

Cited by 7 publications

References 22 publications

Occurrence of Biomphalaria cousini (Mollusca: Gastropoda) in Brazil and its susceptibility to Schistosoma mansoni (Platyhelminths: Trematoda)

Occurrence of Biomphalaria cousini (Mollusca: Gastropoda) in Brazil and its susceptibility to Schistosoma mansoni (Platyhelminths: Trematoda)

A loop-mediated isothermal amplification assay for Schistosoma mansoni detection in Biomphalaria spp. from schistosomiasis-endemic areas in Minas Gerais, Brazil

Phylogenetic relationships and taxonomy of Altiplano populations of Biomphalaria (Gastropoda: Planorbidae): inference from a multilocus approach

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