Morphological and phylogenetic analyses reveal two new species and a new record of Phyllosticta (Botryosphaeriales, Phyllostictaceae) from Hainan, China

Abstract

The fungal genus Phyllosticta has been reported from all around the world and accommodates numerous pathogenic and endophytic species isolated from a wide range of plant hosts. Based on multilocus phylogenies from a combined dataset of genes encoding internal transcribed spacer (ITS), large subunit of ribosomal RNA (LSU rDNA), translation elongation factor 1 alpha (TEF1α), actin (ACT) and glycerol-3-phosphate dehydrogenase (GPDH), in conjunction with morphological characteristics, we describe two new species P. oblongifoliaesp. nov. and P. pterospermisp. nov., as well as a new Chinese record P. capitalensis. Their similarity and dissimilarity to morphologically-allied and phylogenetically-related species are also annotated and discussed.

Phyllosticta taxa from northern Thailand and southern European Russia: a novel species and four new host records

“…previous studies(Wikee et al 2013a;Jayawardena et al 2019;Norphanphoun et al 2020;Zhang et al 2022;Wang et al 2023). However, in our analyses, P. mimusopisicola (CBS 138899) and P. rhodorae (CBS 901.69) clustered in the P. cruenta species complex instead of forming a distinct lineage in the P. rhodorae species complex as shown inNorphanphoun et al (2020).…”

Multi-gene phylogeny and morphology of two new Phyllosticta (Phyllostictaceae, Botryosphaeriales) species from China

“… Phyllosticta is a species-rich genus with more than 3211 records listed in the Index Fungorum ( http://www.indexfungorum.org ). For the Phyllosticta species identification, molecular data have proven useful in resolving species relationships ( Okane et al 2003 ; Su and Cai 2012 ; Guarnaccia et al 2017 ; Norphanphoun et al 2020 ; Zhang et al 2022 ). ITS is a genetic marker for genus level, and combining it with additional loci (LSU, tef1 , act and gapdh ) is enough for species-level resolution ( Jayawardena et al 2019 ; Norphanphoun et al 2020 ).…”

“…Newly generated in this study were combined using SeqMan v. 7.1.0, and reference sequences (Table 1 ) were downloaded from GenBank, according to the recent publication ( Hattori et al 2020 ; Norphanphoun et al 2020 ; Crous et al 2021 ; Bhunjun et al 2022 ; Nguyen et al 2022 ; Tan and Shivas 2022 ; Zhang et al 2022 ). Alignments were done by MAFFT v. 7.036 ( https://maft.cbrc.jp/alignment/server/ ) using default settings and manually improved using MEGA v.7.0 ( Kumar et al 2016 ).…”

“…Based on the phylogenetic analyses of a combined ITS, LSU, tef1 , act and gapdh sequence data, the current taxonomic classification of Phyllosticta comprises six species complexes i.e., P. capitalensis , P. concentrica , P. cruenta , P. owaniana , P. rhodorae and P. vaccinii species complexes ( Norphanphoun et al 2020 ). Currently, the polyphasic approach involving phylogenetic, morphological, and other analyses is used to clarify species boundaries ( Norphanphoun et al 2020 ; Zhang et al 2022 ).…”

“…As endophytes, some species were found associated with leaf spots but did not cause any symptom in pathogenicity tests, e.g., P. oblongifoliae was isolated from leaf spots of Garcinia oblongifolia , P. pterospermi was isolated from leaf spots of Pterospermum heterophyllum , and P. capitalensis was isolated from leaf spots of Citrus spp. ( Wikee et al 2013b ; Tran et al 2019 ; Zhang et al 2022 ). In this study, two novel fungal species named P. anhuiensis and P. guangdongensis , were isolated from diseased leaves of Quercus aliena in Anhui Province and Viburnum odoratissimum in Guangdong Province, respectively.…”