Morphological and molecular studies of a rare mucoralean species causing flower rot in Hylocereus polyrhizus

Sunpapao

2023

JoF

Self Cite

During 2020–2021, cultivated red-fleshed dragon fruit (Hylocereus polyrhizus) in Phatthalung province, southern Thailand, was infected with canker disease in all stages of growth. Small, circular, sunken, orange cankers first developed on the cladodes of H. polyrhizus and later expanded and became gray scabs with masses of pycnidia. The fungi were isolated using the tissue transplanting method and identified based on the growth of the fungal colony, and the dimensions of the conidia were measured. Their species level was confirmed with the molecular study of multiple DNA sequences, and their pathogenicity was tested using the agar plug method. Morphological characterization and molecular identification of the internal transcribed spacer (ITS), translation elongation factor 1-α (tef1-α) and β-tubulin (tub) sequences revealed the fungal pathogen to be a new species. It was named Neoscytalidium hylocereum sp. nov. The biota of the new species, N. hylocereum, was deposited in Mycobank, and the species was assigned accession number 838004. The pathogenicity test was performed to fulfil Koch’s postulates. N. hylocereum showed sunken orange cankers with a mass of conidia similar to those observed in the field. To our knowledge, this is the first report of H. polyrhizus as a host of the new species N. hylocereum causing stem cankers in Thailand.

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A New Species of Neoscytalidium hylocereum sp. nov. Causing Canker on Red-Fleshed Dragon Fruit (Hylocereus polyrhizus) in Southern Thailand

Wonglom

Sunpapao

2023

JoF

Self Cite

“…To test whether fungal isolates can cause disease in rubber tree leaves, pathogenicity testing was conducted according to the agar plug (0.5 mm) method [ 30 , 31 ] to determine fulfillment of Koch’s postulates. Healthy rubber tree leaves were disinfected using 70% ethanol.…”

Section: Methodsmentioning

confidence: 99%

Morphological and Molecular Characterization of Calonectria foliicola Associated with Leaf Blight on Rubber Tree (Hevea brasiliensis) in Thailand

Thaochan

Chairin

et al. 2022

JoF

Leaf blight is commonly observed in rubber trees (Hevea brasiliensis) and can be caused by several fungal species. From October to December 2021, the emergence rubber tree disease was observed in Krabi province, southern Thailand. Small brown to dark brown spots developed on the leaves of rubber trees and later expanded into most parts of the leaves. Fungal isolates were isolated from infected tissues and a total of 15 Calonectria-like isolates were recovered from 10 infected leaf samples. Pathogenicity testing using the agar plug method revealed that four isolates caused leaf blight on rubber tree, similar to the situation in natural infections. Based on morphological study and the molecular properties of internal transcribed spacer (ITS), calmodulin (cal), translation elongation factor 1-α (tef1-α), and β-tubulin 2 (tub2) sequences, the four fungal isolates were identified as Calonectria foliicola. To the best of our knowledge, this is the first report of rubber trees pas a new host for C. foliicola in Thailand and elsewhere. This study reports on an emerging disease affecting rubber trees in Thailand, and the results are of benefit for the development of an appropriate method to manage this emerging disease in Thailand.

“…have been reported to cause canker and bipolaris black spot, respectively, on dragon fruit in Vietnam [5]. Furthermore, the fungus Gilbertella persicaria was recently reported to cause flower rot on red-fleshed dragon fruit in Thailand [6].…”

Section: Introductionmentioning

confidence: 99%

Morphological, Molecular Identification and Pathogenicity of Neoscytalidium dimidiatum Causing Stem Canker of Hylocereus polyrhizus in Southern Thailand

Wonglom

et al. 2022

Plants

Self Cite

Red-fleshed dragon fruit (Hylocereus polyrhizus) is commonly cultivated in Thailand, especially in southern Thailand, where the weather favors plant growth and development. In 2021, stem canker of H. polyrhizus was observed in a dragon fruit plantation field in Phatthalung Province, southern Thailand. Small, orange circular spots developed on the stem of H. polyrhizus, which later became gray, and the lesion expanded with a mass of conidia. Scytalidium-like fungus was isolated from infected tissues. Based on morphology and phylogenetic analyses of internal transcribed spacer (ITS), nuclear large subunit (LSU) and β-tubulin (tub) sequences of fungal isolates, the fungus was identified as Neoscytalidium dimidiatum. Pathogenicity tests revealed that this isolate caused stem canker on the stem of H. polyrhizus, similar to that observed in the field. Knowledge of the diagnosis of plant diseases is an important step for managing plant diseases and therefore, this finding provides basic information for the development of appropriate strategies to manage stem canker disease on H. polyrhizus plants.