Morphological and Molecular (SSR) Analysis of Old Apple Cultivars

Abstract

Investigations were made on the morphological traits of the flowers, shoots and fruits of five apple cultivars in the Batul group and four in the Sóvári Group, in order to prepare UPOV (Union Internationale pour la Protection des Obtentions Végétales) descriptions of these cultivars. Marker analysis involving 12 microsatellite primers was performed to identify 13 genotypes. All the analytical methods indicated that ‘Zöld batul’ could be clearly distinguished from the other ‘Batul’ selections, suggesting that it is an independent cultivar distantly related to ‘Batul’. Within the Sóvári Group, the cultivars could be clearly distinguished from each other on the basis of morphological and molecular analysis. The variants of ‘Beregi sóvári’ collected in the United Kingdom and in Sub-Carpathia cannot be considered to be the same cultivar. The genotype preserved in the UK National Apple Collection at Brogdale should be considered to be an unknown cultivar. The present examinations demonstrated that a combination of morphological and genetic analyses provides a more reliable identification of apple genotypes than pomological descriptions or genetic analysis alone.

DNA Fingerprinting and Genetic Diversity Assessment of GM Cotton Genotypes for Protection of Plant Breeder Rights

“…Moreover, the International Union for the Protection of new Varieties of Plants (UPOV) has encouraged the use of molecular markers in DUS testing for the identification of crop cultivars (UPOV- BMT 2002). Molecular markers are frequently used for effective selection, robust assessment of polymorphism and to explore the relativity of diverse genetic groups of cultivars with their wild relatives (Shah et al 2009;Király et al 2012). Previously, a restricted cotton gene pool has been classified by using Random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP).…”

Valencia ve Navel grup portakal çeşitlerinin SSR markörleri yardımıyla genetik çeşitlilik analizi

“…In later years, PAST has grown into a comprehensive statistics package that is used not only by paleontologists, but also in many fields of life science, earth science, and even engineering and economics (Hammer et al, 2001). PAST program was used in DNA fingerprinting (ISSR and RAPD) of Prosopis cineraria and P.juliflora (Elmeer and Almalki, 2011), molecular (SSR) analysis of old apple cultivars (Király et al, 2012), genetic analysis (RAPD) of Hibiscus species (Kadve et al, 2012), molecular (SSR) determination of genetic structure of Brazilian soybean cultivars (Piriolli et al, 2013), SSR-based genetic diversity assessment in tetraploid and hexaploid wheat populations (Abouzied et al, 2013), molecular diversity (SSR) in cultivated groundnut (Goswami et al, 2013). …”

Genetic diversity of some apple cultivars in the south of Syria based on morphological characters

“…The variance among studied cultivars in leaf characters indicated the importance of leaf characters in discriminating among cultivars which were in agreement with Hajnajari et al (2012) that the leaf characters such leaf length (LL), leaf width (LW) and Petiole length (PL) are important characters in the evaluation of apple cultivars. These characters are usually used in evaluation of apple germplasms beside flower and fruit characters (Damyar et al, 2007;Kiraly et al, 2012). However, Reim et al (2012) depended on the flower and leaf characters and less on the fruit characters for grouping putative M. sylvestris trees.…”