Morphological and molecular phylogenetic analysis of two Saprolegnia sp. (Oomycetes) isolated from silver crucian carp and zebra fish

Species composition of the genus Saprolegnia in fin fish aquaculture environments, as determined by nucleotide sequence analysis of the nuclear rDNA ITS regions

“…The more recent application of molecular genetic techniques (Leclerc et al 2000;Hulvey et al 2007;Ke et al 2009) in combination with morphological and physiological approaches has begun to tackle the challenges associated with the taxonomy of this genus and questions of species identification (Di eguez- ). The latter study integrated information on internal transcribed spacer (ITS) of the nuclear rDNA (nrDNA) nucleotide sequences for a collection of parasitic Saprolegnia spp.…”

“…The combination of highly conserved coding regions (18S, 5.8S, and 28S) separated by the variable, non-coding ITS regions has provided a useful resource for taxonomic and phylogenetic studies (Long & Dawid 1980;Richard et al 2008), allowing resolution of many taxa down to the family or genus level (Oditmann et al 2004;Ballesteros et al 2007), especially when morphological traits were incorporated into the analysis (Molina et al 1995;Chen et al 2000Chen et al , 2001Hulvey et al 2007;Johnson et al 2008;Ke et al 2009). Species-level resolution based on nrDNA amplification alone is not always possible (Chen et al 2000(Chen et al , 2001 and may require restriction fragment length polymorphism (RFLP) analysis for the discrimination of closely related taxa (Molina et al 1995).…”

The in vitro antifungal activity of 30 Chinese herb extracts to Saprolegnia sp.

“…is the most common fungal disease affecting fish and their eggs in fresh and brackish water (Eli et al., ). This disease causes serious losses in fish farms and hatcheries and is a key disease in farmed and wild fish in many countries (Ke et al., ; Karouei, ; Albert et al., ; Vega‐Ramírez et al., ). The hyphae grow through the epidermis and dermis into the musculature and usually cause dermatitis and myositis (Hussein and Hatai, ).…”

Differences in susceptibility to Saprolegnia infections among embryonic stages of two anuran species

“…Oomycetes produce swimming zoospores-which are important for their dispersion in aquatic environments-and are often considered the primary units of infection in many parasitic species (Diéguez-Uribeondo et al 1994). These zoospores are biflagellate and quickly encyst to form primary cysts that can germinate or release a new generation of zoospores (Diéguez-Uribeondo et al 1994Fernández-Benéitez et al 2008;Ke et al 2009;Ghiasi et al 2010). This ability may increase the likelihood of finding an appropriate substratum to germinate and thus ensuring survival (Bangyeekhun et al 2003).…”