Morphological and Molecular Observation to Confirm the Taxonomic of Coptocercus biguttatus (Coleoptera: Cerambycidae) on Cloves in Ambon and Part of Ceram Island

Abstract

This research was conducted to confirm the species of longhorn beetle (Coptocercus biguttatus) drilling clove stems in Ambon and part of Ceram Island, Moluccas, which has been noted as an important pest. Aim of this investigation was to characterize the species morphologically, and more detailed with molecular technique via mtCO1 gene analysis. The longhorn beetle was taken in Ambon and part of Ceram Island, Moluccas and then was etablished in laboratory with host rearing method on pieces of clove stem. The results showed that C. biguttatus attacking clove stems in Ambon and part of Ceram Island, Maluku was closely related and grouped into same cluster with C. rubripes and P. semipunctata in of New Zealand with 85% homology value. C. biguttatus distributed evenly in all clove planting areas in Ambon and part of Ceram Island.

Selecting Primers for RAPD, Microsatellite and Mitochondrial Cytochrome Oxidase Subunit 1 for Genetic Variation Analysis of Asian Corn Borer (Ostrinia furnacalis Guenée) Population in Java, Indonesia

“…The characteristics of mtCO1 primers need to be studied to match the DNA target in ACB, to find out the relationship between samples, and to determine genetic diversity between populations based on the nucleotide diversity in the mtCO1 gene. The mtCO1 primers commonly used are Heb or LCO-HCO for relationship analysis of Orthoptera and Coleoptera (Folmer et al, 1994;Tokuda et al, 2010;Setyolaksono et al, 2017); and Lep or LepF-LepR primers for Lepidoptera, Arctornis moth (Hebert et al, 2004;Sutrisno, 2015). COI primer designed from the complete genome in ACB mitochondria is used to identify the diversity of nucleotides and genetic structures of ACB in China (Li et al, 2014).…”