Morphological and molecular identification of Phytophthora cinnamomi Rands as causal agent of Crown and root rot in Blueberry (Vaccinium corymbosum) in Peru

Abstract

Identificación morfológica y molecular de Phytophthora cinnamomi Rands como agente causal de la pudrición de corona y raíces en arándanos (Vaccinium corymborum) en Perú.

A study of Phytophthora spp. in declining highbush blueberry in Germany reveals that P. cinnamomi can thrive under Central European outdoor conditions

“…Diseases of blueberry, in particular V. corymbosum, caused by P. cinnamomi have long been reported from all over the world. First reports from the U.S. date back to the 1970s (Sterne 1982), and the disease has since been reported from South America, Asia and Europe (Tamietti 2003;Larach et al 2009;Orlikowski et al 2015;Lan et al 2016;Huarhua et al 2018). While in 2010 P. × cambivora was isolated from a young plantation of V. ovata in Germany (Jung et al 2016), so far no incidence of Phytophthora spp.…”

“…Vaccinium corymbosum is a well-known host plant of several Phytophthora species, in particular the notorious P. cinnamomi, causing severe disease and declines all over the world (Sterne 1982;Tamietti 2003;Bryla et al 2008;Larach et al 2009;Orlikowski et al 2015;Lan et al 2016;Huarhua et al 2018). Therefore, an investigation was initiated into the potential involvement of Phytophthora spp.…”

Incidencia de las enfermedades foliares en los sistemas agroforestales de Yurimaguas, Perú

“…The total PCR reaction mix (25 µl) contained 1 µl of genomic DNA (50 to 100 ng / µl), 4 µl of 10X PCR Buffer (ACTaq ™), 2 out of 2.5 mM dNTPs (ACTaq ™), 0.125 µl (5 U) of the enzyme Taq DNA polymerase (ACTaqTM) and 0.5 µl of each primer at a concentration of 20 Mm. The amplification was carried out with a thermal cycler (Thermo Scientific), following the next reaction cycles: initial denaturation of 94 °C for 2 minutes; followed by 35 cycles of denaturation at 98 °C for 1 min, a hybridization temperature at 58 °C for 1 min, elongation of 72 °C for 2 min, and final elongation temperature of 72 °C for 10 min (Ferrer et al, 2001;Kumar & Shukla, 2005;Huarhua et al, 2018;Rep et al, 2004;Inami et al, 2014). The PCR products were separated by 2% agarose gel electrophoresis (0.5 X TAE buffer) containing Hidragreen and run at 90V for 30 minutes.…”