Morphological and molecular identification of the liver fluke Opisthorchis viverrini in the first intermediate host Bithynia snails and its prevalence in Kampong Cham Province, Cambodia

Boonmekam, Dusit; Namchote, Suluck; Matsuda, Hajime; Kirinoki, Masashi; Miyamoto, Kazuko; Muth, Sinuon; Krailas, Duangduen

doi:10.1016/j.parint.2017.01.016

Cited by 5 publications

(5 citation statements)

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“…However, the quantitative morphological characteristics of O. viverrini s.l. cercariae from this study have most structures that were slightly longer and broader than in most previous studies [36,47,63], and more than those described by Boonmekam et al [7] approximately 2-4 times. The latter difference might be because O. viverrini s.l.…”

Section: Discussionsupporting

confidence: 41%

“…infections in Bithynia spp. from various standing-water habitats in northeastern Thailand, Cambodia, Lao PDR, and southern Vietnam were 1.39-3.04%, 0.0-6.8%, and 0.11-1.73%, respectively [7,9,17,27,28,30,32,35,59]. With respect to the populations living in these opisthorchiasis endemic areas, their lifestyles may be associated with higher-prevalence causes than in the present study.…”

Section: Discussionmentioning

confidence: 50%

“…Interestingly, there seem to be consistencies among the relatively high O. viverrini s.l. infections in Bithynia snails, cyprinoid fishes, and humans in northern and northeastern Thailand [27,36,41,55], Cambodia [7,12,32], Lao PDR [11,27,60], and Vietnam [16,17]. Moreover, high to very high O. viverrini s.l.…”

Section: Discussionmentioning

confidence: 99%

“…The identification of samples in this study based on morphological descriptions [23,63] and the detection using species-specific primers [62], which were utilized in previous studies [7, 13, 28-30, 35, 42, 57], and the phylogenetic analysis using the available DNA sequence (i.e., ITS2 region) [7,30,57] The O. viverrini s.l. cercariae from this study appeared indistinguishable based on qualitative morphological characteristics of O. viverrini s.l.…”

Section: Discussionmentioning

confidence: 99%

“…infection in snails reflect the transmission of O. viverrini s.l. from definitive hosts to the environment [7,32], and are useful for forecasting the epidemiological situation of infections in fishes and humans for the development of preventive applications for the future [1,55].…”

Section: Introductionmentioning

confidence: 99%

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Very low prevalence of Opisthorchis viverrini s.l. cercariae in Bithynia siamensis siamensis snails from the canal network system in the Bangkok Metropolitan Region, Thailand

Rachprakhon

Purivirojkul

2021

Parasite

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The liver fluke Opisthorchis viverrini s.l. is associated with a long-term public health problem in Thailand. However, O. viverrini s.l. infection in Bithynia snails in the canal network system (CNS) in the Bangkok Metropolitan Region (BMR) has never been assessed. This study aimed to investigate the occurrence of B. siamensis siamensis and the prevalence of O. viverrini s.l. infection in this snail in the CNS in BMR along with morphological examination and molecular analyses on O. viverrini s.l. cercariae. The snails were randomly sampled from the CNS in all BMR areas from January 2018 to July 2019. Snail specimens were identified and examined for digenean infection by shedding and dissection. The cercariae were identified using morphology and molecular methods, including PCR with a species-specific primer and a Bayesian phylogenetic analysis of ITS2 sequences. Bithynia siamensis siamensis was found in almost all sampling localities, with different quantities and detected frequencies. From a total of 7473 B. s. siamensis specimens, O. viverrini s.l. infections were detected in the Northern Bangkok, Muang Nakhon Pathom, Krathum Baen, and Lam Luk Ka areas with an overall prevalence of 0.05% (4/7473) and prevalence of 0.22% (1/455), 0.21% (1/469), 0.40% (1/253), and 0.16% (1/614) in individual localities with positive snails, respectively. This study is the first investigation of digenean infection in the canal network system-type habitat in Thailand and revealed extremely low O. viverrini s.l. prevalence.

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Section: Discussionsupporting

confidence: 41%