Morphological and molecular identification of hymenolepidid cestodes in children and synanthropic rodents from rural Mexico

Panti–May, Jesús Alonso; Servián, Andrea; Ferrari, Walter; Zonta, María Lorena; Hernández-Mena, David Iván; Hernández-Betancourt, Silvia F.; Robles, María del Rosario; Machaín-Williams, Carlos

doi:10.1016/j.parint.2019.102042

Cited by 10 publications

(9 citation statements)

References 39 publications

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“…We found that H. diminuta had a similar prevalence in our study (8.70%) as it did in a similar study (11.67%) in which these species were observed in rats of Egypt's Assiut Governorate [29]. However, our observed H. diminuta prevalence was lower than that of [31], who reported H. diminuta infection prevalence in 15.3% of R. rattus. In addition, H. nana had a prevalence of 10.4% in our study, which was similar to the prevalence rate of 13.33% reported in the Assiut Governorate [29].…”

Section: Discussionsupporting

confidence: 84%

A parasitological survey of zoonotic cestodes carried by house rats in Aswan, Egypt, reveals cryptic diversity at the molecular level

et al. 2021

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Background and Aim: Some rat cestodes are zoonotic and are capable of parasitizing humans and animals, raising serious concerns regarding human and veterinary health. The study aimed to determine the prevalence and risk factors for cestodes in Egyptian house rats and to characterize the cestodes molecularly. Materials and Methods: The current survey examined 115 house rats (Rattus rattus) in two cities (Edfu and Aswan) in Egypt's Aswan Governorate for cestode infection using integrated molecular approaches (polymerase chain reaction, sequencing, and phylogenetic analysis) and morphological/morphometrical approaches. Results: The cestodes identified in this study exhibited the typical morphological characteristics of Hymenolepis diminuta (Rudolphi, 1819), Hymenolepis nana (Siebold, 1852) (from rat intestine), and Hydatigera taeniaeformis (from rat liver). The species prevalence rates from these three studies were reported to be 8.7%, 10.4%, and 20.9%, respectively. The ribosomal DNA (ITS1, 18S, and complete ITS) sequences revealed that the hymenolepid sequences were highly distinct but were related to other sequences in the GenBank database, with some sequences showing high similarities to those of H. nana and H. diminuta. In addition, the H. taeniaeformis sequences (ITS2 and mitochondrial cytochrome c oxidase subunit 1 [mtCOX1]) obtained in this study were highly similar to some Taenia taeniaeformis GenBank sequences. The constructed phylogram revealed that the hymenolepidid tapeworms examined in this study were classified into four major branches (the majority of which were hybrids of the two species) and belonged to the genus Hymenolepis. In addition, the phylogram of H. taeniaeformis assigned this species to T. taeniaeformis. Conclusion: When typical hymenolepid morphology is combined with molecular and phylogenetic divergence, it may indicate the existence of possible cryptic species. In addition, on the basis of the phylogenetic analysis, genetic diversity within T. taeniaeformis may exist as determined by comparing the metacestode mtCOX1 sequences. The current study presents the prevalence values of zoonotic cestodes and contributes to the body of knowledge, including identification keys and the use of molecular tools for species confirmation.

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Section: Discussionsupporting

confidence: 84%

A parasitological survey of zoonotic cestodes carried by house rats in Aswan, Egypt, reveals cryptic diversity at the molecular level

et al. 2021

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“…The feces were immediately collected from the ground after defecation of macaques and kept cool during transportation. All fecal specimens were washed with distilled water and sieved to eliminate the large sediment from the samples before storage of fecal suspensions at −20°C to perform freeze/thaw cycle of the samples before subjecting to DNA extraction [ 13 ].…”

Section: Methodsmentioning

confidence: 99%

“…Hymenolepis diminuta (rat tapeworm) is a tapeworm of rodents that are present worldwide [ 13 ]. Human infections with this parasite have been reported in 80 countries from 1810 to 2018, including seven cases in Thailand [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

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Molecular identification of Trichuris trichiura and Hymenolepis diminuta in long-tailed macaques (Macaca fascicularis) in Lopburi, Thailand

et al. 2021

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Background and Aim: Trichuris trichiura and Hymenolepis diminuta are helminthic intestinal parasites that infect humans and other animals, including non-human primates. However, molecular detection of these parasites remains scarce in long-tailed macaques (Macaca fascicularis), which coexist with human communities in Thailand. Thus, this study aimed to molecularly confirm the occurrence of Trichuris spp. and Hymenolepis spp. infection and determine the species of both parasites that were found in long-tailed macaques. Materials and Methods: A total of 200 fecal samples were randomly collected from long-tailed macaques living in Lopburi, Thailand, and tested based on polymerase chain reaction (PCR) assays for Trichuris spp. and Hymenolepis spp. infections. The PCR products were submitted for DNA purification and sequencing. Phylogenetic analysis was performed using the maximum likelihood method. Results: Of 200 tested samples, three (1.5%) were positive for Trichuris spp. Sequence analysis of all positive samples revealed the presence of T. trichiura, while eight samples (8/200, 4%) positive for Hymenolepis spp. were classified as H. diminuta. No significant associations were found between parasite infection and sex of macaques. Conclusion: This study revealed that long-tailed macaques harbor T. trichiura and H. diminuta. These results suggested that local residents and tourists must pay attention to limiting contact with long-tailed macaques and take hygienic precautions to reduce the risk of zoonotic and anthroponotic transmission of these parasites between humans and long-tailed macaques.

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“…All H. nana isolates are also known to be infective to humans ( Weinmann, 1966 ). A recent study conducted in Mexico where both H. nana and H. microstoma are known to occur revealed that all hymenolepidid eggs identified in children were H. nana (see Panti-Maya et al., 2020 ). Thus far, H. nana and H. diminuta are the only known truly zoonotic species in the genus Hymenolepis .…”

Section: Hymenolepis Nanamentioning

confidence: 99%

Perspectives on intestinal tapeworm infections: An evaluation of direct and indirect life-cycles with a special emphasis on species of Hymenolepis

Itō

Budke

2021

Current Research in Parasitology & Vector-Borne Diseases

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Morphological and molecular identification of hymenolepidid cestodes in children and synanthropic rodents from rural Mexico

Cited by 10 publications

References 39 publications

A parasitological survey of zoonotic cestodes carried by house rats in Aswan, Egypt, reveals cryptic diversity at the molecular level

A parasitological survey of zoonotic cestodes carried by house rats in Aswan, Egypt, reveals cryptic diversity at the molecular level

Molecular identification of Trichuris trichiura and Hymenolepis diminuta in long-tailed macaques (Macaca fascicularis) in Lopburi, Thailand

Perspectives on intestinal tapeworm infections: An evaluation of direct and indirect life-cycles with a special emphasis on species of Hymenolepis

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