Morphological and molecular identification of Hysterothylacium larvae (Nematoda: Raphidascarididae) in marine fish from Tunisian Mediterranean coasts

Khammassi, Manel; Bahri, Sihem; Pekmezci, Gökmen Zafer

doi:10.1007/s00436-020-06848-4

Cited by 3 publications

(3 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The type V third-stage larvae of Hysterothylacium found in this study showed morphological and morphometric similarities with the larvae described by Bicudo et al ( 2005 2020), collected in several species of marine fish from Brazil and other continents (Shamsi et al 2013, Khammassi et al 2020. The morphotype of the present study, even with a shorter body length, presented morphological structures proportionally similar to the specimens found by the cited authors.…”

Section: Discussionsupporting

confidence: 89%

“…Hysterothylacium Ward & Magath, 1917 includes about 72 valid species being the most abundant and diverse group of ascaridoids parasitic in marine fish, with a worldwide distribution (Moravec & Justine 2015). The identification of these larvae is usually problematic, and different tools for this purpose involving morphological and molecular characterization have been frequently used (Ghadam et al 2018, Jabbar et al 2012, Khammassi et al 2020, Pantoja et al 2016, Roca-Geronès et al 2018, Shamsi et al 2011, 2013, 2020. At least 17 Hysterothylacium larval morphotypes have been described, based mainly on characteristics related to the digestive tract and caudal end, in addition to molecular characterization (Cannon 1977, Shamsi et al 2011.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Morphological and molecular characterization of Hysterothylacium spp. parasitizing Pomatomus saltatrix and Pagrus pagrus of the State of São Paulo, Brazil

Serrano

Vieira

Pelegrini

et al. 2023

An. Acad. Bras. Ciênc.

View full text Add to dashboard Cite

Raphidascarid nematodes have been the focus of several studies, mainly due to the zoonotic potential of some species, even though the cases are underreported. Due to the difficulty in identifying their larvae, the use of diagnostic techniques involving morphological and molecular analyses has grown in the last 20 years. The present study had as objective the morphological and molecular characterization of the L3 larval types of Hysterothylacium collected in Pomatomus saltatrix and Pagrus pagrus from the Brazilian coast, close to the municipality of Santos, State of São Paulo. Twenty specimens of P. saltatrix were necropsied and Hysterothylacium type V (n = 257) and Hysterothylacium type X (n = 5) larvae were found. Five specimens of P. pagrus were necropsied and all were parasitized by Hysterothylacium type V larvae. The analyses showed a genetic proximity relationship between Hysterothylacium types V with other Hysterothylacium V and with H. deardorffoverstreetorum, although this is a species inquirenda. Haplotypes for Hysterothylacium type X found in the present study formed a monophyletic group with other Hysterothylacium X, H. amoyense, and H. zhoushanense.Through this study, new hosts and localities were registered for Hysterothylacium type V and Hysterothylacium type X.

show abstract

Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Morphological and molecular characterization of Hysterothylacium spp. parasitizing Pomatomus saltatrix and Pagrus pagrus of the State of São Paulo, Brazil

Serrano

Vieira

Pelegrini

et al. 2023

An. Acad. Bras. Ciênc.

View full text Add to dashboard Cite

show abstract

“…The genus Anisakis is the most commonly studied; however, other genera might also have equal impact and importance. For example, currently, around 101 species of Hysterothylacium have been described (Bezerra et al 2020) and reported to parasitize marine fish (Shamsi et al 2011;Li et al 2013;Guo et al 2014;Cavallero et al 2015;El-Asely et al 2015;Kong et al 2015;Gazzonis et al 2017;Barcala et al 2018;Chen et al 2018;Khammassi et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Hysterothylacium amoyense (Nematoda: Raphidascarididae) infecting Trichiurus lepturus (Scombriformes: Trichiuridae) from Demak, Central Java, Indonesia

et al. 2022

View full text Add to dashboard Cite

Abstract. Utami AMR, Murwantoko, Istiqomah I, Triyanto, Setyobudi E. 2022. Hysterothylacium amoyense (Nematoda: Raphidascarididae) infecting Trichiurus lepturus (Scombriformes: Trichiuridae) from Demak, Central Java, Indonesia. Biodiversitas 23: 1030-1037. This study aims to determine parasitic infection and identify the larvae of anisakid nematode found on the hairtail Trichiurus lepturus caught on the northern coast of Demak District, Central Java Province, Indonesia. One hundred seventy-eight hairtail samples were collected from fishermen who caught the fish at the Java Sea north coast of Demak. Fish specimens were measured (length) and weighed, then examined for anisakid larvae examination in the abdominal cavity, internal organs, and muscle. Data analysis included prevalence, mean intensity, and distribution of anisakid larvae on target organs. Selected samples of collected anisakid larvae were identified molecularly using PCR direct sequencing method. The results showed that the hairtail at the northern coast of Demak District was susceptible to anisakid larvae infection, on the category "frequently infected" with the prevalence of 56% and low mean intensity of infection of 2.43 larvae/host. Anisakid larvae were mainly found in the abdominal cavity (67%); followed by the digestive tract (19%), liver (13%), and gonads (1%). There were no anisakid larvae infecting the muscle tissue. Molecular analyses identified the anisakid infecting Trichiurus lepturus as Hysterothylacium amoyense.

show abstract

Morphological and molecular characterization of Hysterothylacium larval morphotypes (Nematoda: Raphidascarididae) infecting edible marine fish in the Black Sea

Gelen

Pekmezci

2023

Parasitol Res

View full text Add to dashboard Cite

Morphological and molecular identification of Hysterothylacium larvae (Nematoda: Raphidascarididae) in marine fish from Tunisian Mediterranean coasts

Cited by 3 publications

References 43 publications

Morphological and molecular characterization of Hysterothylacium spp. parasitizing Pomatomus saltatrix and Pagrus pagrus of the State of São Paulo, Brazil

Morphological and molecular characterization of Hysterothylacium spp. parasitizing Pomatomus saltatrix and Pagrus pagrus of the State of São Paulo, Brazil

Hysterothylacium amoyense (Nematoda: Raphidascarididae) infecting Trichiurus lepturus (Scombriformes: Trichiuridae) from Demak, Central Java, Indonesia

Morphological and molecular characterization of Hysterothylacium larval morphotypes (Nematoda: Raphidascarididae) infecting edible marine fish in the Black Sea

Contact Info

Product

Resources

About